NON-INVASIVE BLOOD PHENYLALANINE MONITOR

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 苯丙酮尿症是一种罕见的代谢性疾病，其特征是血液中苯丙氨酸浓度升高。这会导致脑损伤、智力迟钝和神经功能障碍。苯丙氨酸限制饮食治疗可以防止脑损伤。苯丙氨酸血液水平必须保持在一个狭窄的范围内，需要经常监测。该提案的目的是开发一种非侵入性系统来测量苯丙氨酸。我们将比较用类似于Gluco-watch的装置收集的间质液中的苯丙氨酸浓度与血浆苯丙氨酸水平。假设这两种测量结果具有良好的相关性，并且可以开发PKU-手表，以精确和频繁地监测苯丙酮尿症患者的苯丙氨酸水平。 本研究的目的是研究一种新的装置，能够非侵入性地测量苯丙酮尿症患者的苯丙氨酸水平。一种类似于葡萄糖手表的特殊装置将应用于患者 皮肤收集组织液。将在采集的液体中测定苯丙氨酸。还将测定血液中苯丙氨酸的浓度，以确定间质苯丙氨酸与血液水平的相关性。为了进行比较，将同时测量葡萄糖水平。如果成功，该项目将大大提高苯丙酮尿症患者的饮食依从性，改善他们的结果。分析期间将完全使用血样。不考虑建立样本库。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phenylketonuria is a rare metabolic disorder characterized by increased concentrations of phenylalanine in blood. This leads to brain damage, mental retardation, and neurological dysfunction. Treatment with a phenylalanine restricted diet can prevent brain damage. Phenylalanine blood levels must be maintained in a narrow range and require frequent monitoring. The objective of this proposal is to develop a non-invasive system to measure phenylalanine. We will compare phenylalanine concentration in interstitial fluid collected with a device similar to a Gluco-watch to plasma phenylalanine levels. The hypothesis is that the two measurements will have a good correlation and that a PKU-watch can be developed to allow precise and frequent monitoring of phenylalanine levels in patients with phenylketonuria. The objective of this research is to study a new device capable of non-invasively measuring phenylalanine levels in patients with phenylketonuria. A special device, similar to a gluco-watch, will be applied to the patients skin to collect interstitial fluid. Phenylalanine will be measured in the fluid collected. The concentration of phenylalanine will be also determined in blood to determine how interstitial phenylalanine correlates with blood levels. For comparison, glucose levels will be measured simultaneously. If successful this project will greatly improve dietary compliance in patients with phenylketonuria, improving their outcome. Blood samples will be completely utilized during the analysis. No banking of specimen is contemplated.