Organization and plasticity of auditory cortex studied with in vivo 2-photon calc

描述（由申请人提供）：言语和语言的感知需要初级听觉皮层的正常功能。许多疾病导致言语和语言障碍，其中一些可能影响听觉皮层的布线和功能。因此，要了解这些缺陷是如何发展的，并设计新的治疗方法，就必须了解听觉皮层的功能。听觉皮层包含数百万个神经元，其中一个挑战是确定每个神经元对什么样的声音刺激有反应，以及不同反应特性的神经元如何在听觉皮层中组织。我们的研究将揭示听觉皮层是如何组织的，以及复杂的声音刺激（如语音刺激）是如何在听觉皮层中表征的。通过使用先进的成像技术，我们将能够充分考虑皮层区域所有神经元的活动，从而能够更好地理解处理听觉输入的功能电路。通过研究这些回路在发育期间和可塑性任务期间，我们将能够了解听觉皮层的回路是如何变化和成熟的。这是一个至关重要的信息，因为早期外周听觉处理的缺陷会导致皮层回路线路的改变。为了能够纠正这些潜在的错误线路，我们必须了解哪些线路在发育过程中存在，以及它们如何处理感觉信息。我们提出的实验奠定了基础，更好地了解听觉皮层的大规模组织和可塑性的听觉皮层与单细胞分辨率的第一次研究。公共卫生相关性：言语和语言的感知对于人类交流至关重要。许多疾病导致言语和语言障碍，其中一些可能影响听觉皮层的布线和功能。我们提出的实验奠定了基础，更好地了解听觉皮层的大规模组织和可塑性的听觉皮层与单细胞分辨率的第一次研究。

DESCRIPTION (provided by applicant): The perception of speech and language requires normal functioning of the primary auditory cortex. Many disorders lead to speech and language disorders and it is likely that some affect the wiring and function of the auditory cortex. Thus to understand how these deficits develop and to devise novel treatment approaches one has to understand the function of the auditory cortex. The auditory cortex contains millions of neurons and one challenge has been to identify what sound stimulus every neuron is responsive to and how neurons of different response properties are organized in the auditory cortex. Our studies will uncover how auditory cortex is organized and how complex sound stimuli such as speech stimuli are represented in auditory cortex. By using advanced imaging technology we will be able to account fully for the activity of all neurons in a cortical region and thus will be able to better understand the functional circuitry that processes auditory inputs. By studying these circuits over the developmental period and during plasticity tasks we will be able to learn how the circuitry of auditory cortex is changing and maturing. This is crucially important information, since early deficits in peripheral auditory processing can lead to altered wiring of cortical circuits. To be able to correct these potentially miswired circuits we have to understand which circuits are present during development and how they process the sensory information. Our proposed experiments lay the fundamental groundwork for a better understanding of auditory cortex by studying for the first time the large-scale organization and plasticity of auditory cortex with single cell resolution. PUBLIC HEALTH RELEVANCE: The perception of speech and language are of crucial important for human communication. Many disorders lead to speech and language disorders and it is likely that some affect the wiring and function of the auditory cortex. Our proposed experiments lay the fundamental groundwork for a better understanding of auditory cortex by studying for the first time the large-scale organization and plasticity of auditory cortex with single cell resolution.