Genetic Core

遗传核心

• 批准号: 7667781
• 负责人: ROBERT DAVID SCHREIBER
• 金额: $ 20.78万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7667781
• 关键词: Animals; Backcrossings; Basic Science; Biological; Cryopreservation; Derivation procedure; Disease model; ES Cell Line; Embryo; Equipment; Experimental Models; Fibroblasts; Funding; Genes; Genetic; Genome; Genotype; Human Resources; Inbred Strain; Individual; Knock-out; Knockout Mice; Laboratories; Mus; Procedures; Process; Production; Proteins; Purpose; Relative (related person); Rheumatism; Role; Scientist; Services; Speed; System; Techniques; Transgenic Organisms; blastocyst; congenic; experimental analysis; genetic manipulation; genetic technology; genome sequencing; insight; mouse model; mutant; skills

Genetic manipulation of mouse models has been an extremely productive approach to studying basic biological mechanisms. A Genetics Core has been established and has been productive during the last funding period. The RDCC proposes to continue support of the Genetics Core to provide two major services: 1) Transgenic and knockout mouse production and 2) Speed congenics. The transgenic and knockout mouse production facility provides additional services including blastocyst complementation, derivation of new embryonic stem cell lines, cryopreservation, and mouse embryonic fibroblast production. The speed congenics facility provides genome wide typing for the purposes of enhancing backcrossing of mice into defined genetic backgrounds. Thus, the facility will enhance the study of basic mechanisms underlying rheumatic diseases.

对小鼠模型进行遗传操作是研究基础疾病的一种极具成效的方法