BIOMARKERS RESOURCE

由癌症中心和哥伦比亚NIEHS中心共同管理的生物标志物共享资源 为北方曼哈顿的环境健康提供了一个集中的，有效的和具有成本效益的 用于接收、处理和储存作为研究一部分采集的人体样本的资源 分子流行病学和其他类型的癌症相关研究。它是巨蟹座的共同资源 中心和哥伦比亚的NIEHS环境健康中心在北方曼哈顿。提供的服务 包括以下步骤： 生物样本采集试剂盒的制备 血液成分的处理和储存，包括细胞和血浆 ●储存其他体液，包括尿液、痰液和口腔细胞 通过质量控制分离DNA <$样品库存的维护 高通量至中等通量定制单核苷酸多态性基因分型 为特定研究提供样本采集和处理的所有方面的咨询，包括 样品采集所需的试管类型、运输方法、特定组分的分离， 处理和/或储存的样本包括血液、尿液、口腔细胞、痰液和 涂片细胞或石蜡切片的显微镜载玻片。机构收到编码样本， 加工成适当的级分（例如，血浆、单核细胞、粒细胞、总白色血细胞 和红细胞），并尽可能在多个冷冻器中以多个等分试样冷冻。一个网上 维护样本库存的数据库，并按照 管理协议。电话报警系统用于确保样品安全。附加服务包括 从口腔和白色血细胞中分离DNA，对有限的DNA样品进行全基因组扩增，以及 候选基因中单核苷酸多态性的高通量基因分型。样品几乎来自 参与50项不同研究的24，000名受试者，其中43项与癌症相关，目前储存在 生物标志物实验室与几个新的研究开始在当前的赠款周期。未来的计划包括 将患者招募扩大到其他癌症部位，扩大可用的冷冻空间， 随着可靠和具有成本效益的新技术的不断更新， available.业务预算为388 000美元，其中我们要求CCSG提供87 730美元。

The Biomarkers Shared Resource, jointly managed by the Cancer Center and Columbia's NIEHS Center for Envoronmental Health in Northern Manhattan provides a centralized, efficient and cost-effective resource for receiving, handling and storing human samples that are collected as part of research studies in molecular epidemiology and other types of cancer-related research. It is a joint resource of the Cancer Center and Columbia's NIEHS Center for Environmental Health in Northern Manhattan. Services provided include: ¿ Preparation of kits for biospecimen collection ¿ Processing and storage of blood components, including cells and plasma ¿ Storage of other body fluids, including urine, sputum, and oral cells ¿ Isolation of DNA with quality control ¿ Maintenance of a sample inventory ¿ High to medium-throughput custom single nucleotide polymorphism genotyping Consultation is provided on all aspects of sample collection and processing for specific studies including the types of tubes needed for sample collection, shipment methods, separation of specific fractions, number of aliquots, etc. The samples processed and/or stored include blood, urine, oral cells, sputum and microscope slides of smeared cells or paraffin sections. Coded samples are received by the facility and processed into appropriate fractions (e.g., plasma, mononuclear cells, granulocytes, total white blood cells and red blood cells) and frozen in multiple aliquots in more that one freezer whenever possible. A webbased data base of the sample inventory is maintained and samples retrieved as as specified in the governing protocol. A telephone alarm system is used to ensure sample safety. Additional services include DNA isolation from buccal and white blood cells, whole genome amplification of limited DNA samples, and high throughput genotyping for single nucleotide polymorphisms in candidate genes. Samples from almost 24,000 subjects participating in 50 different studies, 43 cancer-related, are currently stored in the Biomarkers Laboratory with several new studies initiated in the current grant cycle. Future plans include expansion of patient recruitment to additional cancer sites, expansion of the available freezer space and continual updating of genotyping platforms as reliable and cost-effective new technologies become available. The operating budget is $388,000, of which we are requesting $87,730 from the CCSG.