BIOMARKERS RESOURCE

生物标记物共享资源，由癌症中心和哥伦比亚大学NIEHS中心联合管理 为曼哈顿北部的环境健康提供集中、高效和经济实惠的 用于接收、处理和存储作为研究研究一部分收集的人体样本的资源 在分子流行病学和其他类型的癌症相关研究中。它是癌症的联合资源 中心和哥伦比亚大学位于曼哈顿北部的NIEHS环境健康中心。提供的服务 包括： ？生物样品采集试剂盒的研制 ？血液成分的加工和储存，包括细胞和血浆 ？储存其他体液，包括尿液、痰液和口腔细胞 DNA的提取及质量控制 ？维护样本清单 高中量定制单核苷酸多态基因分型 就特定研究的样本收集和处理的所有方面提供咨询，包括 样品采集所需的管子类型、运输方法、特定组分的分离、 处理和/或存储的样本包括血液、尿液、口腔细胞、痰和 涂片细胞或石蜡切片的显微镜载玻片。编码样本由该设施接收，并且 加工成适当的组分(例如，血浆、单核细胞、粒细胞、总白细胞 和红细胞)，并尽可能在一个以上的冷冻箱中以多等份的形式冷冻。基于Web的 维护样品清单的数据库，并按照 治理协议。采用电话报警系统，确保样品安全。其他服务包括 从口腔和白细胞中分离DNA，对有限的DNA样本进行全基因组扩增，以及 候选基因单核苷酸多态的高通量基因分型。几乎所有的样本 参与50项不同研究的24,000名受试者，其中43名与癌症有关，目前存储在 生物标志物实验室，在当前的赠款周期中启动了几项新的研究。未来的计划包括 将患者招募扩大到更多的癌症地点，扩大可用的冷藏空间和 随着可靠和具有成本效益的新技术的出现，基因分型平台不断更新 可用。业务预算为388,000美元，其中我们要求协调小组提供87,730美元。

The Biomarkers Shared Resource, jointly managed by the Cancer Center and Columbia's NIEHS Center for Envoronmental Health in Northern Manhattan provides a centralized, efficient and cost-effective resource for receiving, handling and storing human samples that are collected as part of research studies in molecular epidemiology and other types of cancer-related research. It is a joint resource of the Cancer Center and Columbia's NIEHS Center for Environmental Health in Northern Manhattan. Services provided include: ¿ Preparation of kits for biospecimen collection ¿ Processing and storage of blood components, including cells and plasma ¿ Storage of other body fluids, including urine, sputum, and oral cells ¿ Isolation of DNA with quality control ¿ Maintenance of a sample inventory ¿ High to medium-throughput custom single nucleotide polymorphism genotyping Consultation is provided on all aspects of sample collection and processing for specific studies including the types of tubes needed for sample collection, shipment methods, separation of specific fractions, number of aliquots, etc. The samples processed and/or stored include blood, urine, oral cells, sputum and microscope slides of smeared cells or paraffin sections. Coded samples are received by the facility and processed into appropriate fractions (e.g., plasma, mononuclear cells, granulocytes, total white blood cells and red blood cells) and frozen in multiple aliquots in more that one freezer whenever possible. A webbased data base of the sample inventory is maintained and samples retrieved as as specified in the governing protocol. A telephone alarm system is used to ensure sample safety. Additional services include DNA isolation from buccal and white blood cells, whole genome amplification of limited DNA samples, and high throughput genotyping for single nucleotide polymorphisms in candidate genes. Samples from almost 24,000 subjects participating in 50 different studies, 43 cancer-related, are currently stored in the Biomarkers Laboratory with several new studies initiated in the current grant cycle. Future plans include expansion of patient recruitment to additional cancer sites, expansion of the available freezer space and continual updating of genotyping platforms as reliable and cost-effective new technologies become available. The operating budget is $388,000, of which we are requesting $87,730 from the CCSG.