RADIATION RESEARCH RESOURCE

一个完整的癌症中心管理的设施，辐射研究共享资源位于和 由放射学研究中心维护。该设施提供全面的辐照 主要服务于中国人民银行会员和其他会员。提供的主要服务有： 细胞和动物模型的X射线照射 细胞和动物模型的伽马射线照射 紫外线照射细胞和动物模型 对用户进行实验设计和辐照器安全操作方面的培训 在过去的六年里，对该设施的需求稳步增长， 自2000年以来，用户实验室增加了230%（64对28）。辐射共享资源 能够将样品暴露于X射线、伽马射线和紫外线的机器。样品范围从 小的大分子如DMA和蛋白质，到微生物、培养的哺乳动物细胞或小的 动物可以暴露。研究目标包括这样的任务，如创造双链断裂或庞大的 DMA中的损伤，在注射细胞之前照射小鼠以测试致瘤性，产生转化的细胞， 制备用于组织培养的饲养层，并测量酵母、小鼠、仓鼠或 人体细胞该设施有四个辐照器，一个西门子X射线治疗单位，一个加拿大原子能 Gammacell 40铯装置、高剂量率Gammacell 220钴装置和发射254 nm光。该设施的操作员为用户提供安全和有效的样品暴露指导， 以及实验设计咨询。该设施的运作与 辐射安全部确保符合政府监管机构的要求。未来计划 包括制造用于培养中细胞的亚细胞条纹照射的装置，另一个 能够在X射线照射期间旋转动物以允许内部解剖结构照射的装置 目标，同时限制暴露于周围组织，并与比较研究所 医学研究所开发一种成像系统，用于将辐射瞄准解剖子区域， 小鼠癌症模型。 在CCSG的最后一个阶段，使用该设施的研究者中有39%是癌症中心 成员与同行评审的资金，这些成员代表约60%的使用， 的主要服务。该设施的拟议业务预算总额为110，022美元，其中我们是 向CCSG申请61,612美元

A full Cancer Center-managed facility, the Radiation Research Shared Resource is located within and maintained by the Center for Radiological Research. The facility provides a comprehensive irradiation service for members of the HICCC primarily and others. The primary services provided are: ¿ X-ray irradiation of cells and animal models ¿ Gamma-ray irradiation of cells and animal models ¿ UV irradiation of cells and animal models ¿ Training of users in experimental design and safe operation of the irradiators Demand for the facility has steadily increased during the last six years, where the number of independent user laboratories increased by 230% since 2000 (64 versus 28). The Radiation Shared Resource has machinery capable of exposing samples to x-rays, gamma-rays and ultraviolet light. Samples ranging from small macromolecules such as DMA and proteins, to microorganisms, mammalian cells in culture or small animals can be exposed. Research goals include such tasks as creating double strand breaks or bulky lesions in DMA, irradiating mice before injection of cells to test tumorigenicity, creating transformed cells, preparing feeder layers for tissue culture, , and measuring radiosensitivity of yeast, mouse, hamster or human cells. The facility has four irradiators, a Siemans X-ray Therapy Unit, an Atomic Energy of Canada Gammacell 40 Cesium Unit, a high-dose-rate Gammacell 220 Cobalt Unit, and a UV light box emitting 254 nm light. The operators of the facility provide users with guidance for safe and efficient sample exposure, as well as consultation on experimental design. The facility is operated in close association with the Radiation Safety Department to ensure compliance with governmental regulatory agencies. Future plans include the manufacture of an apparatus for subcellular stripe irradiation of cells in culture, another apparatus capable of rotating animals during x-ray exposure to permit irradiation of an internal anatomical target while limiting exposure to surrounding tissues, and working with the Institute for Comparative Medicine at CUMC to develop an imaging system for targeting radiation to anatomical sub-regions in mouse models of cancer. During the last period of the CCSG, 39% of the investigators using the facility were Cancer Center members with peer-reviewed funding with those members representing approximately 60% of the usage of the primary services. The proposed total operating budget of the facility is $110,022, of which we are requesting $ 61,612 from the CCSG.