RADIATION RESEARCH RESOURCE

辐射研究资源

• 批准号: 7669925
• 负责人: HOWARD B. LIEBERMAN
• 金额: $ 5.42万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7669925
• 关键词: Animal Model; Animals; Boxing; Budgets; Canada; Cancer Center; Cancer Center Support Grant; Carcinogenicity Tests; Cataract; Cells; Cesium; Cobalt; Consultations; DNA Damage; Dose-Rate; Ensure; Environment; Equipment; Experimental Designs; Exposure to; Eye; Funding; Future; Gamma Rays; Goals; Hamsters; Human; Image; Injection of therapeutic agent; Institutes; Laboratories; Lesion; Light; Malignant Neoplasms; Mammalian Cell; Measures; Medicine; Mus; Nuclear Energy; Numbers; Operative Surgical Procedures; Peer Review; Proteins; Radiation; Radiation Tolerance; Range; Research; Research Personnel; Resource Sharing; Resources; Roentgen Rays; Safety; Sampling; Secure; Security; Services; Signal Transduction Pathway; System; Tissues; Training; Ultraviolet Rays; Universities; Work; X-Ray Therapy; Yeasts; cell transformation; comparative; irradiation; macromolecule; member; microorganism; mouse model; repaired; tissue culture; ultraviolet irradiation

A full Cancer Center-managed facility, the Radiation Research Shared Resource is located within and maintained by the Center for Radiological Research. The facility provides a comprehensive irradiation service for members of the HICCC primarily and others. The primary services provided are: ¿ X-ray irradiation of cells and animal models ¿ Gamma-ray irradiation of cells and animal models ¿ UV irradiation of cells and animal models ¿ Training of users in experimental design and safe operation of the irradiators Demand for the facility has steadily increased during the last six years, where the number of independent user laboratories increased by 230% since 2000 (64 versus 28). The Radiation Shared Resource has machinery capable of exposing samples to x-rays, gamma-rays and ultraviolet light. Samples ranging from small macromolecules such as DMA and proteins, to microorganisms, mammalian cells in culture or small animals can be exposed. Research goals include such tasks as creating double strand breaks or bulky lesions in DMA, irradiating mice before injection of cells to test tumorigenicity, creating transformed cells, preparing feeder layers for tissue culture, , and measuring radiosensitivity of yeast, mouse, hamster or human cells. The facility has four irradiators, a Siemans X-ray Therapy Unit, an Atomic Energy of Canada Gammacell 40 Cesium Unit, a high-dose-rate Gammacell 220 Cobalt Unit, and a UV light box emitting 254 nm light. The operators of the facility provide users with guidance for safe and efficient sample exposure, as well as consultation on experimental design. The facility is operated in close association with the Radiation Safety Department to ensure compliance with governmental regulatory agencies. Future plans include the manufacture of an apparatus for subcellular stripe irradiation of cells in culture, another apparatus capable of rotating animals during x-ray exposure to permit irradiation of an internal anatomical target while limiting exposure to surrounding tissues, and working with the Institute for Comparative Medicine at CUMC to develop an imaging system for targeting radiation to anatomical sub-regions in mouse models of cancer. During the last period of the CCSG, 39% of the investigators using the facility were Cancer Center members with peer-reviewed funding with those members representing approximately 60% of the usage of the primary services. The proposed total operating budget of the facility is $110,022, of which we are requesting $ 61,612 from the CCSG.

辐射研究共享资源是一个由癌症中心管理的完整设施，位于和 由放射研究中心维护。该设施提供全面的辐射 主要为HICCC成员和其他成员提供服务。提供的主要服务包括： 细胞和动物模型的X射线照射 细胞和动物模型的伽玛射线照射 细胞和动物模型的紫外线照射 对用户进行实验设计和辐照器安全操作方面的培训 在过去的六年中，对该设施的需求稳步增长，其中独立的 自2000年以来，用户实验室增加了230%(与28个)。辐射共享资源具有 能够将样品暴露在X射线、伽马射线和紫外线下的机器。样品的范围从 小分子如DMA和蛋白质，可用于微生物、哺乳动物细胞的培养或小 动物可能会被暴露。研究目标包括创造双链断裂或体积较大的任务 DMA的损害，在注射细胞之前照射小鼠以测试致瘤性，创造转化的细胞， 为组织培养准备饲养层，并测量酵母、小鼠、仓鼠或 人类细胞。该设施有四个辐照器，一个西门子X射线治疗仪，加拿大原子能公司 Gammacell 40铯单元、高剂量率Gammacell 220钴单元和发射254的UV灯箱 NM灯。该设施的操作员为用户提供安全和有效的样品暴露指南， 以及关于实验设计的咨询。该设施的运营与 辐射安全部确保遵守政府监管机构的规定。未来计划 包括制造用于对培养中的细胞进行亚细胞条带照射的设备，另 能够在X射线曝光期间旋转动物以允许照射内部解剖结构的装置 目标，同时限制暴露于周围组织，并与比较研究所合作 加州大学医学院将开发一种成像系统，用于将辐射定向到 癌症的小鼠模型。 在CCSG的最后一段时间里，使用该设施的调查人员中有39%是癌症中心 获得同行审查资金的成员，这些成员约占 主要服务。该设施的拟议总运营预算为110,022美元，其中我们 向CCSG索要61,612美元。