RADIATION RESEARCH RESOURCE

辐射研究资源

基本信息

批准号：
7669925
负责人：
HOWARD B. LIEBERMAN
金额：
$ 5.42万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-08-01 至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7669925
关键词：
Animal Model Animals Boxing Budgets Canada Cancer Center Cancer Center Support Grant Carcinogenicity Tests Cataract Cells Cesium Cobalt Consultations DNA Damage Dose-Rate Ensure Environment Equipment Experimental Designs Exposure to Eye Funding Future Gamma Rays Goals Hamsters Human Image Injection of therapeutic agent Institutes Laboratories Lesion Light Malignant Neoplasms Mammalian Cell Measures Medicine Mus Nuclear Energy Numbers Operative Surgical Procedures Peer Review Proteins Radiation Radiation Tolerance Range Research Research Personnel Resource Sharing Resources Roentgen Rays Safety Sampling Secure Security Services Signal Transduction Pathway System Tissues Training Ultraviolet Rays Universities Work X-Ray Therapy Yeasts cell transformation comparative irradiation macromolecule member microorganism mouse model repaired tissue culture ultraviolet irradiation

项目摘要

A full Cancer Center-managed facility, the Radiation Research Shared Resource is located within and maintained by the Center for Radiological Research. The facility provides a comprehensive irradiation service for members of the HICCC primarily and others. The primary services provided are: ¿ X-ray irradiation of cells and animal models ¿ Gamma-ray irradiation of cells and animal models ¿ UV irradiation of cells and animal models ¿ Training of users in experimental design and safe operation of the irradiators Demand for the facility has steadily increased during the last six years, where the number of independent user laboratories increased by 230% since 2000 (64 versus 28). The Radiation Shared Resource has machinery capable of exposing samples to x-rays, gamma-rays and ultraviolet light. Samples ranging from small macromolecules such as DMA and proteins, to microorganisms, mammalian cells in culture or small animals can be exposed. Research goals include such tasks as creating double strand breaks or bulky lesions in DMA, irradiating mice before injection of cells to test tumorigenicity, creating transformed cells, preparing feeder layers for tissue culture, , and measuring radiosensitivity of yeast, mouse, hamster or human cells. The facility has four irradiators, a Siemans X-ray Therapy Unit, an Atomic Energy of Canada Gammacell 40 Cesium Unit, a high-dose-rate Gammacell 220 Cobalt Unit, and a UV light box emitting 254 nm light. The operators of the facility provide users with guidance for safe and efficient sample exposure, as well as consultation on experimental design. The facility is operated in close association with the Radiation Safety Department to ensure compliance with governmental regulatory agencies. Future plans include the manufacture of an apparatus for subcellular stripe irradiation of cells in culture, another apparatus capable of rotating animals during x-ray exposure to permit irradiation of an internal anatomical target while limiting exposure to surrounding tissues, and working with the Institute for Comparative Medicine at CUMC to develop an imaging system for targeting radiation to anatomical sub-regions in mouse models of cancer. During the last period of the CCSG, 39% of the investigators using the facility were Cancer Center members with peer-reviewed funding with those members representing approximately 60% of the usage of the primary services. The proposed total operating budget of the facility is $110,022, of which we are requesting $ 61,612 from the CCSG.

一个完整的癌症中心管理的设施，放射研究共享资源位于内部和由放射学研究中心维护。该设施提供了全面的辐照为HICCC小学和其他人的成员提供服务。提供的主要服务是：细胞和动物模型的X射线照射细胞和动物模型的伽马射线照射 »细胞和动物模型的紫外线照射 „对辐照器的实验设计和安全操作的用户培训在过去的六年中，对该设施的需求悄悄增加，独立的数量自2000年以来，用户实验室增长了230％（64对28）。辐射共享资源具有能够将样品暴露于X射线，伽马射线和紫外线的机械。样品不等小型大分子，例如DMA和蛋白质，用于微生物，培养或小的哺乳动物细胞动物可以暴露。研究目标包括诸如创建双链断裂或笨重的任务 DMA中的病变，在注射细胞之前辐照小鼠以测试肿瘤性，形成转化的细胞，制备用于组织培养的进食层，并测量酵母，小鼠，仓鼠或人类细胞。该设施有四个辐照器，一个西曼X射线治疗单元，加拿大的原子能 Gammacell 40 Cesium单元，高剂量速率伽玛克220钴单元和紫外线灯盒发射254 NM光。该设施的运营商为用户提供了安全有效的样品暴露的指南，以及有关实验设计的咨询。该设施与辐射安全部门确保遵守政府监管机构。未来计划包括制造用于培养物细胞亚细胞条纹辐射的设备，另一种 X射线暴露期间能够旋转动物的设备，以允许辐射内部解剖学目标同时限制暴露于周围的组织，并与研究所合作 CUMC的医学开发成像系统，以靶向辐射到解剖学子区域癌症的小鼠模型。在CCSG的最后一个时期，使用该设施的39％的研究人员是癌症中心由同行评审的成员与这些成员约有60％的使用主要服务。该设施的拟议总运营预算为110,022美元，我们是向CCSG要求$ 61,612。