RADIATION RESEARCH RESOURCE

辐射研究资源

• 批准号: 7669925
• 负责人: HOWARD B. LIEBERMAN
• 金额: $ 5.42万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7669925
• 关键词: Animal Model; Animals; Boxing; Budgets; Canada; Cancer Center; Cancer Center Support Grant; Carcinogenicity Tests; Cataract; Cells; Cesium; Cobalt; Consultations; DNA Damage; Dose-Rate; Ensure; Environment; Equipment; Experimental Designs; Exposure to; Eye; Funding; Future; Gamma Rays; Goals; Hamsters; Human; Image; Injection of therapeutic agent; Institutes; Laboratories; Lesion; Light; Malignant Neoplasms; Mammalian Cell; Measures; Medicine; Mus; Nuclear Energy; Numbers; Operative Surgical Procedures; Peer Review; Proteins; Radiation; Radiation Tolerance; Range; Research; Research Personnel; Resource Sharing; Resources; Roentgen Rays; Safety; Sampling; Secure; Security; Services; Signal Transduction Pathway; System; Tissues; Training; Ultraviolet Rays; Universities; Work; X-Ray Therapy; Yeasts; cell transformation; comparative; irradiation; macromolecule; member; microorganism; mouse model; repaired; tissue culture; ultraviolet irradiation

A full Cancer Center-managed facility, the Radiation Research Shared Resource is located within and maintained by the Center for Radiological Research. The facility provides a comprehensive irradiation service for members of the HICCC primarily and others. The primary services provided are: ¿ X-ray irradiation of cells and animal models ¿ Gamma-ray irradiation of cells and animal models ¿ UV irradiation of cells and animal models ¿ Training of users in experimental design and safe operation of the irradiators Demand for the facility has steadily increased during the last six years, where the number of independent user laboratories increased by 230% since 2000 (64 versus 28). The Radiation Shared Resource has machinery capable of exposing samples to x-rays, gamma-rays and ultraviolet light. Samples ranging from small macromolecules such as DMA and proteins, to microorganisms, mammalian cells in culture or small animals can be exposed. Research goals include such tasks as creating double strand breaks or bulky lesions in DMA, irradiating mice before injection of cells to test tumorigenicity, creating transformed cells, preparing feeder layers for tissue culture, , and measuring radiosensitivity of yeast, mouse, hamster or human cells. The facility has four irradiators, a Siemans X-ray Therapy Unit, an Atomic Energy of Canada Gammacell 40 Cesium Unit, a high-dose-rate Gammacell 220 Cobalt Unit, and a UV light box emitting 254 nm light. The operators of the facility provide users with guidance for safe and efficient sample exposure, as well as consultation on experimental design. The facility is operated in close association with the Radiation Safety Department to ensure compliance with governmental regulatory agencies. Future plans include the manufacture of an apparatus for subcellular stripe irradiation of cells in culture, another apparatus capable of rotating animals during x-ray exposure to permit irradiation of an internal anatomical target while limiting exposure to surrounding tissues, and working with the Institute for Comparative Medicine at CUMC to develop an imaging system for targeting radiation to anatomical sub-regions in mouse models of cancer. During the last period of the CCSG, 39% of the investigators using the facility were Cancer Center members with peer-reviewed funding with those members representing approximately 60% of the usage of the primary services. The proposed total operating budget of the facility is $110,022, of which we are requesting $ 61,612 from the CCSG.

放射研究共享资源是一个由癌症中心管理的完整设施，位于 由放射学研究中心维护。该设施提供全面的照射 主要为 HICCC 成员和其他成员提供服务。提供的主要服务有： ¿ 细胞和动物模型的X射线照射 ¿ 细胞和动物模型的伽马射线照射 ¿ 细胞和动物模型的紫外线照射 ¿ 对用户进行辐射器实验设计和安全操作方面的培训 在过去六年中，对该设施的需求稳步增加，独立的数量 自 2000 年以来，用户实验室增加了 230%（64 个对 28 个）。辐射共享资源有 能够将样品暴露于 X 射线、伽马射线和紫外线的机器。样本范围从 小大分子，如 DMA 和蛋白质，对微生物、培养物中的哺乳动物细胞或小分子 动物可能会暴露。研究目标包括创建双链断裂或大体积等任务 DMA 中的损伤，在注射细胞之前照射小鼠以测试致瘤性，产生转化细胞， 准备用于组织培养的饲养层，并测量酵母、小鼠、仓鼠或 人体细胞。该设施有四个辐照器、一个西门子 X 射线治疗装置、一个加拿大原子能装置 Gammacell 40 铯装置、高剂量率 Gammacell 220 钴装置和发射 254 的紫外线灯箱 纳米光。该设施的操作员为用户提供安全有效的样品暴露指导， 以及实验设计咨询。该设施的运营与 辐射安全部门确保遵守政府监管机构的规定。未来计划 包括制造用于对培养物中的细胞进行亚细胞条纹照射的装置，另一个 能够在 X 射线照射期间旋转动物以允许内部解剖结构受到照射的装置 目标，同时限制与周围组织的接触，并与比较研究所合作 CUMC 医学部将开发一种成像系统，用于将辐射定向到以下区域的解剖分区： 小鼠癌症模型。 在 CCSG 的最后一个时期，使用该设施的研究人员中有 39% 来自癌症中心 拥有同行评审资金的成员，这些成员约占使用量的 60% 主要服务。该设施的拟议总运营预算为 110,022 美元，其中我们 向 CCSG 请求 61,612 美元。