Protein Structure Core

自1988年以来，蛋白质结构核心一直是UM-MAC/RDCC的一部分。我们计划 继续为RDCC提供及时和经济高效的最新蛋白质生物技术 成员由我们在项目规划、数据分析和培训方面的经验支持。我们将维持我们的 高度重视与RDCC调查人员的互动。虽然调查人员可以自由提交服务请求 在不进行讨论的情况下，我们鼓励与核心主任举行会议，目的是高效和务实 计划，并在项目结束时进行数据分析。 多肽合成是我们目前最常要求的服务，我们预计这将继续下去 保持不变。我们合成了线型多肽以及多标记多肽、环多肽和多肽。 模仿翻译后的修饰。可以订购具有定制特性的多肽，这些特性包括 天然氨基酸或修饰的非天然结构元素(生物素或其他亲和标记，荧光染料， 供体-受体对，构象限制，磷酸盐)用于结构-功能研究，免疫学 检测、抗体生产、细胞内转运和其他应用。将合成多肽类化合物 使用FMOC-固相法。多肽合成与反相高效液相色谱联用纯化 多肽，和质谱分析，以确认正确的合成。 我们还将继续提供目前可用的蛋白质分析服务，包括Edman Sequence 分析(识别N-末端序列标签，补充LC/MS分析)、氨基酸分析 (外包，用于鉴定未知蛋白质，以确定在LC/MS之前的蛋白质分解策略 分析，并定量蛋白质和表征MAP多肽)和圆二色谱(确定 蛋白质二级结构、构象稳定性和多肽修饰的构象效应)。 通过增加Waters LC/MS UPLC Qtof，我们的蛋白质分析能力将得到极大的提高 高级串联质谱仪，将与生物信息学分析结合使用，用于 多肽-质量指纹图谱和串联质谱学鉴定蛋白质。我们还将利用 该仪器鉴定蛋白质翻译后修饰的灵敏度和分辨率 (尤其是磷酸化)，这是生物医学研究中一个日益重要的领域。

The Protein Structure Core has been part of the UM-MAC/RDCC continuously since 1988. We plan to continue to provide timely and cost-efficient access to state-of-the-art protein biotechnology for RDCC members backed up by our exptertise in project planning, data analysis, and training. We will maintain our strong focus on interaction with RDCC investigators. While investigators are free to submit service requests without discussion, we encourage meetings with the Core Director for the purpose of efficient and practical planning, and for data analysis at the conclusion of a project. Peptide synthesis is currently our most frequently requested service and we expect that this will continue unchanged. We synthesize linear peptides as well as multiply labeled peptides, cyclic peptides and peptides mimicking post-translational modifications. Peptides can be ordered with tailored characteristics containing natural amino acids or modified unnatural structural elements (biotin or other affinity labels, fluorochromes, donor-acceptor pairs, conformational constraints, phosphates) for structure-function studies, immunological assays, antibody production, intracellular trafficking, and other applications. Pepetides will be synthesized using Fmoc-solid phase methodology. Peptide synthesis is coupled with RP-HPLC based purification of peptides, and mass spectroscopic analysis to confirm correct synthesis. We will also continue to offer currently available services in protein analysis, including Edman sequence analysis (to identify N-terminal sequence tags, complementing LC/MS analysis), amino acid analysis (outsourced, used to characterize unknown proteins to determine strategies for proteolysis before LC/MS analysis, and to quantify proteins and characteize MAP peptides), and circular dichroism (to determine protein secondary structure, conformational stability, and conformational effects of peptide modification). Our capacity in protein analysis will be vastly improved by the addition of a Waters LC/MS UPLC Qtof Premier Tandem mass spectrometer, which will be used in conjuction with bioinformatics analysis, for identification or proteins by peptide-mass -fingerprinting and tandem mass spectroscopy. We will also utilize the sensitivity and resolution of this instrument for identification of post-translational modification of proteins (especially phosphorylation), an increasingly important area in biomedical research.