Protein Structure Core

蛋白质结构核心

• 批准号: 8139100
• 负责人: HENRIETTE Anna REMMER
• 金额: $ 5.38万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8139100
• 关键词: Affinity Labels; Amino Acids; Antibody Formation; Area; Arts; Back; Bioinformatics; Biological Assay; Biomedical Research; Biotechnology; Biotin; Characteristics; Circular Dichroism; Circular Dichroism Spectroscopy; Complement; Core Protein; Coupled; Custom; Cyclic Peptides; Data Analyses; Elements; Fingerprint; Fluorochrome; Goals; Label; Mass Spectrum Analysis; Methodology; Michigan; Modification; N-terminal; Outsourcing; Peptide Synthesis; Peptides; Phase; Phosphorylation; Post-Translational Protein Processing; Protein Analysis; Proteins; Proteolysis; Research; Research Personnel; Resolution; Rheumatism; Secondary Protein Structure; Sequence Analysis; Services; Solid; Structure; Training; Universities; Water; affinity labeling; base; cost; improved; inorganic phosphate; instrument; liquid chromatography mass spectrometry; mass spectrometer; meetings; member; protein structure; trafficking

The Protein Structure Core has been part of the UM-MAC/RDCC continuously since 1988. We plan to continue to provide timely and cost-efficient access to state-of-the-art protein biotechnology for RDCC members backed up by our exptertise in project planning, data analysis, and training. We will maintain our strong focus on interaction with RDCC investigators. While investigators are free to submit service requests without discussion, we encourage meetings with the Core Director for the purpose of efficient and practical planning, and for data analysis at the conclusion of a project. Peptide synthesis is currently our most frequently requested service and we expect that this will continue unchanged. We synthesize linear peptides as well as multiply labeled peptides, cyclic peptides and peptides mimicking post-translational modifications. Peptides can be ordered with tailored characteristics containing natural amino acids or modified unnatural structural elements (biotin or other affinity labels, fluorochromes, donor-acceptor pairs, conformational constraints, phosphates) for structure-function studies, immunological assays, antibody production, intracellular trafficking, and other applications. Pepetides will be synthesized using Fmoc-solid phase methodology. Peptide synthesis is coupled with RP-HPLC based purification of peptides, and mass spectroscopic analysis to confirm correct synthesis. We will also continue to offer currently available services in protein analysis, including Edman sequence analysis (to identify N-terminal sequence tags, complementing LC/MS analysis), amino acid analysis (outsourced, used to characterize unknown proteins to determine strategies for proteolysis before LC/MS analysis, and to quantify proteins and characteize MAP peptides), and circular dichroism (to determine protein secondary structure, conformational stability, and conformational effects of peptide modification). Our capacity in protein analysis will be vastly improved by the addition of a Waters LC/MS UPLC Qtof Premier Tandem mass spectrometer, which will be used in conjuction with bioinformatics analysis, for identification or proteins by peptide-mass -fingerprinting and tandem mass spectroscopy. We will also utilize the sensitivity and resolution of this instrument for identification of post-translational modification of proteins (especially phosphorylation), an increasingly important area in biomedical research.

自1988年以来，蛋白质结构核心一直是UM-MAC/RDCC的一部分。我们计划 继续为RDCC提供及时和具有成本效益的最新蛋白质生物技术 我们在项目规划、数据分析和培训方面的专业知识为会员提供支持。我们将保持我们 非常注重与RDCC调查人员的互动。虽然调查人员可以自由提交服务请求， 在没有讨论的情况下，我们鼓励与核心主任举行会议，以实现高效和实用的目的。 规划，并在项目结束时进行数据分析。 多肽合成是我们目前最经常要求的服务，我们希望这将继续下去 不变我们合成线性肽以及多标记肽，环肽和肽 模仿翻译后修饰。肽可以定制的特点，包括 天然氨基酸或修饰的非天然结构元件（生物素或其它亲和标记，荧光染料， 供体-受体对，构象约束，磷酸盐）用于结构-功能研究，免疫学 测定、抗体生产、细胞内运输和其他应用。将合成Peptides 使用Fmoc-固相方法。肽合成与基于RP-HPLC的纯化偶联， 肽和质谱分析以确认正确的合成。 我们还将继续提供目前可用的蛋白质分析服务，包括Edman测序 分析（鉴定N-末端序列标签，补充LC/MS分析），氨基酸分析 （外包，用于表征未知蛋白质，以确定LC/MS前的蛋白水解策略 分析，并定量蛋白质和表征MAP肽），和圆二色性（以确定 蛋白质二级结构、构象稳定性和肽修饰的构象效应）。 通过添加沃茨LC/MS UPLC Qtof，我们的蛋白质分析能力将得到极大提高 Premier Tandem质谱仪将与生物信息学分析结合使用， 通过肽质量指纹和串联质谱鉴定蛋白质。我们还将利用 该仪器用于鉴定蛋白质翻译后修饰灵敏度和分辨率 （特别是磷酸化），在生物医学研究中越来越重要的领域。