Core C Mass Spectrometry Core

质谱学核心将为DPPG提供现代质谱学的强大工具 调查人员。用GC/MS对脂类和氧化氨基酸进行定量仍然是 核心。核心还将允许高密度脂蛋白(HDL)的结构识别和定量- 相关生物分子，包括载脂蛋白A-L和血清淀粉样蛋白。在过去的两年里，Core 工作人员也精通二维液相色谱-电喷雾电离- 蛋白质的复杂生物混合物的MS/MS分析(鸟枪式蛋白质组学)，我们预计所有 调查人员将在下一个资金期利用这一能力。 除了提供仪器能力外，质谱学核心员工还将提供咨询和 在将质谱学应用于DPPG研究计划方面的合作，这将包括 开发新的分析方法，以实现DPPG调查人员的研究目标。 质谱学核心将优化效率和成本效益，通过 通过提供中心实验室，向DPPG调查人员提供服务。这就避免了需要 独立的DPPG调查人员在自己的实验室维护所需的仪器，并避免 商业质谱学服务的高昂成本。通过集中和标准化程序， 质谱学核心将提供一套通用的分析工具，将导致统一的 对糖尿病生理和病理生理过程分子机制的认识 血管疾病。

The Mass Spectrometry Core will provide the powerful tools of modern mass spectrometry to DPPG investigators. Quantification of both lipids and oxidized amino acids by GC/MS remains a mainstay of the Core. The Core will also permit structural identification and quantitation of high density lipoprotein (HDL)- related biomolecules, including apolipoprotein A-l and serum amyloid protein. Over the past two years, Core personnel have also become proficient in 2-dimensional liquid chromatography-electrospray ionization- MS/MS analysis (shotgun proteomics) of complex biological mixtures of proteins, and we anticipate that all investigators will take advantage of this capability in the next funding period. In addition to providing instrumental capabilities, Mass Spectrometry Core staff will provide consultation and collaboration on the application of mass spectrometry to the DPPG research program, and this will include development of new analytical methods targeted at achieving research objectives of DPPG investigators. The Mass Spectrometry Core will optimize the efficiency and cost-effectiveness through which these services are provided to DPPG investigators by providing a central laboratory. This avoids the need for individual DPPG investigators to maintain the required instrumentation in their own laboratories and avoids the high cost of commercial mass spectrometric services. By centralizing and standardizing procedures, the Mass Spectrometry Core will provide a common set of analytical tools that will lead to a unified understanding of molecular mechanisms involved in physiologic and pathophysiologic processes of diabetic vascular disease.