Quantitative Assessment of HDL Function

HDL 功能的定量评估

• 批准号: 8989561
• 负责人: JAY W HEINECKE
• 金额: $ 43.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8989561
• 关键词: ATP binding cassette transporter 1; Acute; Address; Affinity; Animal Model; Anti-Inflammatory Agents; Anti-inflammatory; Antiatherogenic; Apolipoprotein A-I; Apolipoproteins A; Arteries; Atherosclerosis; Behavior; Biochemical; Biological Assay; Biological Markers; Cardiovascular Diseases; Carotid Artery Diseases; Cause of Death; Cholesterol; Complement; Computing Methodologies; Enrollment; Genes; Goals; High Density Lipoprotein Cholesterol; High Density Lipoproteins; Human; Inflammation; Inflammatory; Isotopes; Lead; Lipids; Magnetic Resonance Imaging; Measures; Mediator of activation protein; Membrane; Pathway interactions; Peroxidases; Population; Post-Translational Protein Processing; Process; Property; Prospective Studies; Proteins; Proteome; Proteomics; Qualifying; Research; Resources; Serum; Shotguns; Societies; Sterols; Study Subject; Testing; Therapeutic; acute coronary syndrome; cardiovascular disorder risk; cholesterol control; citrate carrier; design; improved; in vivo; macrophage; mouse model; novel; novel diagnostics; novel therapeutic intervention; oxidation; oxidative damage; programs; prospective; response; reverse cholesterol transport

This application is submitted in response to PA-11-012-Toward an improved understanding of HDL- which states "The ultimate goal of this FOA is to develop reproducible and robust assays to measure HDL function and to identify novel genes and pathways related to HDL function." We believe we are well-qualified to address this need because our research program has focused on understanding the factors that contribute to the cardioprotective effects of HDL. Two key components of our approach have centered on (i) investigating the factors that control cholesterol efflux and HDL's ability to inhibit macrophage inflammation, and (ii) developing mass spectrometric approaches for quantifying oxidation products and proteins in HDL. Importantly, we have identified specific mechanism that may impair the cardioprotective effects of HDL. One involves oxidative damage of apoA-I (the major HDL protein) by myeloperoxidase (MPO). MPO impairs apoA-I's ability to remove cholesterol from macrophages by the ABCA1 pathway. Another potential mechanism involves alterations in the anti-inflammatory proteins that are carried by HDL. Using shotgun proteomics, we have demonstrated that HDL carries a unique cargo of proteins in cardiovascular disease (CVD) subjects and that those proteins might make previously unsuspected contributions to HDL's function. Moreover, we have shown that HDL of subjects with CVD or acute inflammation exhibts impaired ability to remove cholesterol from macrophages, the key first step in reverse cholesterol transport. Our studies will take advantage of three unique human populations. The first involves control subjects, subjects with acute coronary syndrome, and subjects with established CVD. The second involves control subjects and subjects with CVD enrolled in a prospective trial of statin therapy. The third population took part in a prospective study of subjects with carotid artery disease that was evaluated for atherosclerotic progression by MRI. The availability of these valuable resources will enable us to investigate specific mechanisms for generating dysfunctional HDL in subjects with CVD, the leading cause of death in industrialized societies.

本申请是为了响应pa -11-012-对HDL-的更好理解而提交的