Morphology Core

形态学和成像核心将提供动物模型中动脉粥样硬化的分析， 使用传统和新技术测量范围、进展速度和组成。 此外，它将提供多种成像技术，可用于体外和体内分析， 实验模型这个核心将是位于斯克里普斯的单位之间的合作努力 在琳达柯蒂斯博士的指导下，一个位于旧金山加州大学的研究所 迭戈在尤里米勒医生手下 核心的总体目标是为每个单元的研究人员提供传统和新颖的 技术，以评估在小鼠模型中动脉粥样硬化的程度以及进展，以提供 技术，以允许表征病变，并提供各种成像技术，在这两个有用的 体外和体内实验模型。该核心的目标是： 具体目标1：提供小鼠模型中动脉粥样硬化程度的测量。这将 包括使用贯穿主动脉长度的区域的标准正面测量、使用 计算机辅助技术，以提供更标准化的病灶负荷体积测量， 主动脉窦，以及使用激光扫描测量早期病变进展率的新方法 共聚焦免疫荧光法提供原位评估主动脉巨噬细胞的定量技术 浸润 具体目标2：提供组织和细胞形态的定性和定量成像分析。 这将包括使用激光扫描共聚焦显微镜研究，以提供新的定性图像， 三维，病变在不同的发展阶段，以及使用去卷积 显微镜检查以提供培养物中的病变或细胞内的形态学的定性和定量分析。 具体目标3：验证激光捕获显微切割的使用，以允许基因的显微分析 在病变中或在病变内的限定细胞组内表达。

The Morphology and Imaging Core will provide for analyses of atherosclerosis in animal models, providing measures of extent, rate of progression and composition using both conventional and novel technologies. In addition it will provide a variety of imaging techniques useful in both in vitro and in vivo analyses of experimental models. This Core will be a collaborative effort between units located at The Scripps Research Institute under the direction of Dr. Linda Curtiss and one located at University California, San Diego under Dr. Yury Miller. The overall goal of the Core will be to provide investigators in each of the units both conventional and novel techniques to assess both the extent as well as progression of atherosclerosis in murine models, to provide techniques to allow characterization of lesions, and to provide a variety of imaging techniques useful in both in vitro and in vivo experimental models. The Aims of this Core are: Specific Aim 1: To provide measurements of the extent of atherosclerosis in murine models. This will include use of standard en face measurements of areas throughout the length of the aorta, the use of a computer assisted technique to provide more standardized volume measurements of lesion burden at the aortic sinus, and use of'a novel measurement of rate of early lesion progression using laser scanning confocal immunofluorescence to provide a quantitative technique to asses in situ aortic macrophage infiltration. Specific Aim 2: To provide qualitative and quantitative imaging analysis of tissue and cellular morphology. This will include use of laser scanning confocal microscopy studies to provide novel qualitative images, in three dimension, of lesions at various stages of development, as well as the use of deconvolution microscopy to provide qualitative and quantitative analysis of morphology within lesions or cells in culture. Specific Aim 3: To validate the use of laser capture microdissection to allow microanalysis of gene expression in lesions or within groups of defined cells within lesions.