SUR1 mutations and Inter-Subunit Associations In ATP-Sensitive Potassium Channels
ATP 敏感钾通道中的 SUR1 突变和亚基间关联
基本信息
- 批准号:7585170
- 负责人:
- 金额:$ 4.41万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-03-01 至 2012-02-29
- 项目状态:已结题
- 来源:
- 关键词:ATP sensitive potassium channel complexAddressAffectAmino Acid SubstitutionAmino AcidsB-LymphocytesBeta CellBiochemicalBiogenesisBiological AssayBiologyBlood GlucoseCategoriesCell membraneCell physiologyCellsChargeChemicalsClassificationCo-ImmunoprecipitationsComplementComplexCouplingCysteineDataDefectDevelopmentDiabetes MellitusDiseaseDisulfidesEventExposure toGoalsHomology ModelingHydrophobicityInsulinIon ChannelLeadLigandsLinkMeasuresMetabolicMolecular ChaperonesMutagenesisMutateMutationNatureNucleotidesPancreasPersistent Hyperinsulinemia Hypoglycemia of InfancyPharmaceutical PreparationsPotassiumProcessPropertyProteinsPublic HealthRecoveryReportingResearchResolutionScanningSeveritiesSignal TransductionSpecific qualifier valueStructureStructure-Activity RelationshipSulfonylurea CompoundsTestingTransmembrane DomainWestern BlottingWorkanalogbasechemical propertydisease-causing mutationdrug sensitivityfallsinsightinsulin secretionmembermutantneonatal diabetes mellitusoxidationprospectiveprotein foldingresearch studysulfonylurea receptortrafficking
项目摘要
DESCRIPTION (provided by applicant): The goal of this research is to understand how the subunits of ATP sensitive potassium channels (KATP) interact by utilizing known disease-causing mutations as starting points for biochemical and electrophysiological analysis. KATP channels are expressed in and responsible for beta-cell physiology. Their conductance state is regulated by intracellular nucleotide levels, and thus is an important link between cellular metabolic status and cell excitability. KATP channels are composed of two types of subunits: Kir6.2 and SUR1, But how these subunits communicate with each other-their specific inter-subunit amino acid interactions, and how these interactions specify KATP biogenesis and activity-is not known. High- resolution crystal structures do not exist for either subunit or for the KATP complex; therefore, functional experiments are needed to answer these important questions. I will perform a detailed study of two residues within SUR1, E128 and R74. These residues are associated with congenital hyperinsulinism due to trafficking defects, but their activity profiles mimic neonatal diabetes mutations. Both residues are members of a select group, mutation of which leads to trafficking defects that can be 'rescued' by sulfonylurea (SU) treatment. Based on the fact that mutation of either residue leads to KATP biogenesis and activity defects, I hypothesize that E128 and R74 contribute to SUR1-Kir6.2 interactions. Specific Aim 1 addresses how these two residues facilitate proper KATP biogenesis using biochemical experiments. I will determine how systematic mutagenesis of these residues effect SUR1-Kir6.2 associations (co-immunoprecipitation) and trafficking to the plasma membrane (western blot & chemiluminescence). Further, the influence of SU on both processes will be measured. Specific Aim 2 utilizes the same mutant SUR1 subunits and considers how they affect KATP channel activity, both ATP and SU sensitivities. Finally, in Specific Aim 3,1 will identify the residues of Kir6.2 that interact with SUR1 E128 and R74 by a systematic mutagenesis scan of Kir6.2. Verification of SUR1-Kir6.2 interacting pairs will be achieved by study of double-mutant electrophysiological properties and the ability of prospective pairs to form disulphide-bridges following cysteine-replacement and oxidation. This work is of particular public health importance because drugs widely used in diabetes treatment, sulfonylureas, have recently been found to change an important regulator of insulin secretion, the KATP ion channel. Studying how these drugs work has the potential for discovery of new treatments for a different disease, congenital hyperinsulinism. In addition, this work will further our understanding of the basic biology of the KATP channel.
描述(由申请人提供):本研究的目标是通过利用已知的致病突变作为生化和电生理分析的起点,了解ATP敏感钾通道(KATP)亚基如何相互作用。KATP通道在β细胞生理中表达并负责。它们的电导状态受细胞内核苷酸水平的调控,因此是细胞代谢状态和细胞兴奋性之间的重要联系。KATP通道由Kir6.2和SUR1两种亚基组成,但这些亚基如何相互通信-它们特定的亚基间氨基酸相互作用,以及这些相互作用如何指定KATP的生物发生和活性-尚不清楚。无论是亚基还是KATP复合物都不存在高分辨率的晶体结构;因此,需要功能性实验来回答这些重要的问题。我将对SUR1, E128和R74中的两个残基进行详细的研究。这些残基与先天性高胰岛素血症有关,但它们的活性谱与新生儿糖尿病突变相似。这两种残基都是一个选择组的成员,其突变导致可通过磺脲(SU)处理“拯救”的运输缺陷。基于任一残基突变均可导致KATP生物发生和活性缺陷的事实,我假设E128和R74参与了SUR1-Kir6.2的相互作用。具体目标1解决如何这两个残基促进适当的KATP生物发生使用生化实验。我将确定这些残基的系统诱变如何影响SUR1-Kir6.2关联(共免疫沉淀)和质膜运输(western blot和化学发光)。此外,还将测量SU对这两个过程的影响。特异性Aim 2利用相同的突变体SUR1亚基,并考虑它们如何影响KATP通道活性,包括ATP和SU敏感性。最后,在Specific Aim 3中,我将通过对Kir6.2进行系统突变扫描,确定Kir6.2中与SUR1 E128和R74相互作用的残基。SUR1-Kir6.2相互作用对的验证将通过研究双突变电生理特性和预期对在半胱氨酸替代和氧化后形成二硫桥的能力来实现。这项工作具有特殊的公共卫生重要性,因为最近发现广泛用于糖尿病治疗的药物磺脲类药物可以改变胰岛素分泌的重要调节因子,即KATP离子通道。研究这些药物如何起作用,有可能发现治疗另一种疾病——先天性高胰岛素血症的新方法。此外,这项工作将进一步加深我们对KATP通道基本生物学的理解。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Emily B Pratt其他文献
Emily B Pratt的其他文献
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{{ truncateString('Emily B Pratt', 18)}}的其他基金
SUR1 mutations and Inter-Subunit Associations In ATP-Sensitive Potassium Channels
ATP 敏感钾通道中的 SUR1 突变和亚基间关联
- 批准号:
7487588 - 财政年份:2008
- 资助金额:
$ 4.41万 - 项目类别:
SUR1 mutations and Subunit Associations in ATP-Sensitive Potassium Channels
ATP 敏感钾通道中的 SUR1 突变和亚基关联
- 批准号:
8037030 - 财政年份:2008
- 资助金额:
$ 4.41万 - 项目类别:
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