Molecular diversity in olfactory wiring specificity

嗅觉线路特异性的分子多样性

• 批准号: 7545864
• 负责人: Maria L Spletter
• 金额: $ 3.84万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7545864
• 关键词: Axon; Birth Order; Brain; Classification; Dendrites; Development; Drosophila genus; Embryo; Fingers; In Situ Hybridization; Individual; Lobe; Maps; Mediating; Modeling; Molecular; Mutation; Nerve; Neurons; Olfactory Receptor Neurons; Phenotype; Protein Isoforms; RNA; RNA Splicing; Smell Perception; Specificity; System; Testing; Transgenes; axon guidance; base; gene function; homologous recombination; mutant; olfactory receptor; overexpression; transcription factor

The Drosophila olfactory system presents a great opportunity to understand the general principles of wiring specificity, and how the wiring of neuronal circuits contributes to olfaction. In the Drosophila olfactory system, second order projection neurons (PNs) innervate specific glomeruli in the antennal lobe. We are exploring the molecular mechanisms by which PNs target their dendrites to specific glomeruli. Longitudinals lacking (lola) is a Zn-finger transcription factor identified as a putative regulator of axon guidance that splices to at least 20 unique isoforms. This proposal will further investigate the function of individual Lola isoforms in PN wiring specificity. The lola locus may prove a useful model to demonstrate the function of molecular diversity at the transcriptional level in wiring specificity. Specifically, I will 1. Perform a systematic expression analysis of individual lola isoforms in the AL using isoform specific RNA in-situ hybridization and immunostaining. 2. Generate new isoform-specific mutants for those isoforms expressed in PNs using homologous recombination, and test their function in PN wiring using MARCM analysis. 3. Generate isoform-specific UAS transgenes to test their ability to rescue isoform-specific mutants using MARCM mediated overexpression.

果蝇嗅觉系统提供了一个很好的机会来了解接线的一般原则 特异性以及神经元电路的接线如何促进嗅觉。在果蝇嗅觉系统中， 二阶投射神经元（PNS）支配触角叶中的特定肾小球。我们正在探索 PNS将其树突靶向特定肾小球的分子机制。纵向缺乏 （LOLA）是Zn指转录因子，被确定为轴突指导的推定调节剂，可拼接至AT 至少20个独特的同工型。该建议将进一步研究PN单个Lola同工型的功能 接线特异性。 LOLA基因座可能证明是一个有用的模型来证明分子多样性的功能 在接线特异性的转录级别上。具体来说，我将1。执行系统的表达分析 使用同工型特异性RNA静脉杂交和免疫染色的单个LOLA同工型。 2。 使用同源物生成新的同工型特异性突变体 重组，并使用MARCM分析测试其在PN接线中的功能。 3。生成同工型特异性UA 转基因使用MARCM介导的过表达来测试其拯救同工型特异性突变体的能力。

项目成果

Lola regulates Drosophila olfactory projection neuron identity and targeting specificity.

• DOI: 10.1186/1749-8104-2-14
• 发表时间: 2007-07-16
• 期刊: Neural development
• 影响因子: 3.6
• 作者: Spletter ML;Liu J;Liu J;Su H;Giniger E;Komiyama T;Quake S;Luo L
• 通讯作者: Luo L

A new family of odorant receptors in Drosophila.

果蝇中的一个新的气味受体家族。

• DOI: 10.1016/j.cell.2008.12.031
• 发表时间: 2009
• 期刊: Cell
• 影响因子: 64.5
• 作者: Spletter,MariaL;Luo,Liqun
• 通讯作者: Luo,Liqun