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Genetic mechanisms controlling the visual pathway to the central complex of the Drosophila brain

控制果蝇大脑中央复合体视觉通路的遗传机制

基本信息

批准号：
9896874
负责人：
VOLKER HARTENSTEIN
金额：
$ 32.97万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-04-01 至 2022-03-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9896874
关键词：
Address Adhesions Anatomy Anterior Ants Axon Bees Behavior Binding Biological Models Birth Birth Order Brain Candidate Disease Gene Cell Communication Cells Characteristics Complex Data Development Drosophila genus Elements Exhibits Eye Gene Expression Genes Genetic Genetic Markers Genetic study Human Image Individual Insecta Interneurons Knock-out Libraries Location Locomotion Maps Modeling Molecular Molecular Genetics Monitor Mus Nervous system structure Neurons Optic Lobe Optics Pathway interactions Pattern Perception Phenotype Play Process Property RNA Interference Recording of previous events Regulator Genes Reporter Research Role Semaphorins Shapes Sorting - Cell Movement Specific qualifier value Stereotyping Structure Synapses System Techniques Testing Therapeutic Time Vision Visual Visual Pathways Walking Work base brain circuitry cell type differential expression embryonic stem cell experimental study fly genetic analysis knock-down locomotor control locust mind control neuroblast neuronal circuitry orientation selectivity polarized light preservation progenitor public health relevance response sensory stimulus spatial relationship stem cells time interval tool transcriptome transcriptome sequencing two-photon virtual visual information visual stimulus

项目摘要

DESCRIPTION (provided by applicant): The studies of this application ask how gene expression controls neuronal connectivity and, thereby, brain function. This question is of general importance if one wants to understand, and (therapeutically) manipulate brain circuitry. We use the model system Drosophila, where virtually every gene can be targeted for knock-out or activation in a cell type selective manner Drosophila also offers the advantage that its brain i composed of a relatively small number of stereotyped neuronal lineages, groups of neurons descended from individual embryonic stem cells, called neuroblasts. During the course of its proliferation, each neuroblast expresses characteristic sets of regulatory genes. These genes control the differentiation of the neurons born from that particular neuroblast during a particular time interval. Through this mechanism, a lineage, or smaller subdivision of a lineage called sublineage, develops into a specific class of neurons which share common wiring properties, including the projection of their axons, branching pattern, and placement of synapses. Several discrete neuronal classes/lineages are put together into a neuronal circuit. We have identified a circuit, called the anterior visual pathway (AVP), which conducts input form the eye to a brain center, the central complex, known to process and store visual information in order to control fly locomotion (walking, flight). The central part of this circuit is formed by three lineages, whose neurons form several classes of highly ordered parallel and sequential elements. In our first aim we will investigate the function of the neuronal classes of the AVP, by recording their activity in response to defined visual stimuli. We will also demonstrate experimentally that these classes of neurons are directly connected by synapses. The second aim addresses the question how the developmental history of a neuron (time of birth, placement within the spatial framework of the developing brain) relates to its later connectivity within the AVP circuit. Furthermore, by genetically ablating specific classes of AVP neurons and monitoring the response of their normal synaptic partners, we will obtain important clues towards the role of specific cell interactions ordering connectivity. Thirdly, using high throughput RNAseq, we will analyze the complete assortment of genes (transcriptome) expressed differentially in two particular AVP sublineages, R3 and R2. These two classes are distinguished from each other by very few structural criteria, and we will screen for and then analyze genes responsible for their differences in wiring. We expect to identify genes which play a general role in controlling pathway choices and connectivity in the nervous system.