Molecular and Biochemical Core - Greengard (pgs. 236 - 251)

尽管经过了几十年的研究，精神分裂症仍然是一种知之甚少的神经系统疾病。然而， 临床和实验研究表明，多巴胺能、多巴胺能和多巴胺能神经传递在 the pathophysiology病理生理学of this debilitating衰弱psychiatric精神disorder紊乱.更好地理解细胞内信号 与这些神经递质有关的诱导机制可能是治疗这种疾病的更有效的方法。的 分子和生物化学核心的职责将是为许多研究提供基础， 在项目1-5中提出的抗精神病药物行动。核心将执行各种任务， 将确定新的蛋白质-蛋白质相互作用的基础分子生物学和常规生物化学，这将有助于 调查这些研究。核心还将为执行日常任务提供技术支持设施 以有效和成本效益的方式完成项目1-5中描述的研究所需的资源。特希 任务将是维持纯化蛋白激酶、蛋白磷酸酶、亲和纯化抗体的库存， 底物蛋白将使用标准纯化方案从天然来源获得这些酶，并且 重组技术也将用于生产特定的蛋白质，其数量足以进行详细的研究。 酶学和结构研究。核心还将监督新的多角体的生产和测试核心还将监督新的多角体的生产和测试 抗肽抗体和磷酸化状态特异性抗体。

Despite many decades of study, schizophrenia remains a poorly understood neurologicaldisorder.However, clinical and experimental studies have implicated dopaminergic, serotoriergic, and glutamatergicneurotransmission in the pathophysiology of this debilitatingpsychiatric disorder. A better understandingof the intracellular signal Iransduction mechanisms linked to these neurotransmitters may more effective treatments for this disease. The responsibilities of the Molecular and Biochemical Core will be to provide a foundation for many of the studies of neuroleptic drug action proposed in Projects 1-5. The Core will perform tasks ranging from sophisticatedscreens that will identify novel protein-protein interactions to basic molecular biology and routine biochemistry that will aid in the investigation these studies. The Core will also provide a technical support facility for the performanceof routine tasks that will be required to accomplish the studies described in Projects 1-5 in an efficient and cosl-effective manner. Thesi tasks will be to maintain stocks of purified protein kinases, protein phosphatases, affinity-purified antibodies, and substrate proteins. Standard purification protocols will be used to obtain these enzymes from native sources, and recombinant technologies will also be employed to produce specific proteins in quantities sufficient to carry out detailed enzymological and structural studies. The Core will also supervise the productionand testing of new polydonal anti-peptide antibodies and phosphorylation state-specific antibodies.