NUTRITIONAL AND FUNCTIONAL METHIONNE REQUIREMENTS IN SEPTIC ADOLESCENTS

脓毒症青少年的营养和功能性蛋硫氨酸需求

• 批准号: 7950611
• 负责人: Leticia Castillo
• 金额: $ 0.44万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7950611
• 关键词: Adolescent; Amino Acids; Anabolism; Child; Childhood; Choline; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; Creatine; Critical Illness; Critically ill children; DNA Methylation; Data; Disease Marker; Equilibrium; Functional disorder; Funding; Goals; Gold; Grant; Homocysteine; Homocystine; Institution; Intake; Lymphocyte; Metabolism; Methionine; Newborn Infant; Nutritional; Nutritional Requirements; Outcome; Peripheral; Population; Production; Protein Biosynthesis; Research; Research Personnel; Resources; Severities; Source; Sulfur Amino Acids; Techniques; Time; United States National Institutes of Health; base; improved; oxidation; septic; sound

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This portocol is a continuation of our studies on sulfur amino acid metabolism in critically ill septic children. The immediate objectives of this project are to better define sulfur amino acid requirements in the healthy and in the critically ill septic pediatric population from newborn to adolescent. This is an ambitious goal that can only be approached with one project at a time. In this proposal we are focused exclusively in the healthy and critically ill adolescent population. Amino acid requirements in healthy children are based on extremely limited data. The amino acid requirements for critically ill children in therms of nutritional and functional balance are not known. Methionine, an indispensable sulfur amino acid serves for protein synthesis in addition to DNA methylation and biosynthesis of important compounds such as creatine, choline, etc. However, an excessive methionine administration may contribute to endothelial dysfunction of critical illness through an excessive production of homocysteine. In the present studies we propose to define nutritional requirements of methionine and to begin to explore functional requirements in 196 critically ill septic adolescents and 196 healthy adolescents over a period of 5 years. We propose to modify the "gold" standard techniqure of amino acid requirement determination, the 24h-indicator amino acid oxidation and balance technique, to make it applicable to both, the healthy and critically ill populations. We plan also to correlate methionine intake with severity of disease, markers of endothelial dysfunction and rates of DNA methylation in peripheral lymphocytes. Our long-term aim is to eventually determine the amino acid requirements that will maintain nutritional balance and function in the pediatric population from newborn to adolescents, to improve outcome in the sick population and to establish sound nutritional standards based on quantitative, reliable data in the healthy pediatric population.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这篇文章是我们对重症脓毒症儿童含硫氨基酸代谢研究的继续。 本项目的近期目标是更好地确定从新生儿到青少年的健康和重症脓毒症儿科人群的含硫氨基酸需求。 这是一个雄心勃勃的目标，一次只能通过一个项目来实现。 在这项提案中，我们专门关注健康和重症青少年人群。 健康儿童的氨基酸需求量基于非常有限的数据。 危重患儿在营养和功能平衡方面的氨基酸需求量尚不清楚。 蛋氨酸是一种不可或缺的含硫氨基酸，除了DNA甲基化和肌酸、胆碱等重要化合物的生物合成外，还用于蛋白质合成。然而，过量的蛋氨酸给药可能通过过量产生同型半胱氨酸而导致危重病的内皮功能障碍。 在目前的研究中，我们建议确定蛋氨酸的营养需求，并开始探索功能需求在196个危重败血症青少年和196个健康青少年在一个为期5年。 我们建议修改确定氨基酸需要量的“金”标准技术，即24小时指示剂氨基酸氧化和平衡技术，使其既适用于健康人群，也适用于危重人群。 我们还计划将甲硫氨酸摄入量与疾病的严重程度、内皮功能障碍的标志物和外周淋巴细胞DNA甲基化率相关联。 我们的长期目标是最终确定维持新生儿到青少年儿科人群营养平衡和功能的氨基酸需求，改善患病人群的结局，并根据健康儿科人群的定量可靠数据建立合理的营养标准。