NHLBI DNA Re-sequencing and Genotyping Program

NHLBI DNA 重测序和基因分型计划

• 批准号: 7693490
• 金额: $ 39.99万
• 依托单位: J. CRAIG VENTER INSTITUTE, INC.
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7693490
• 关键词: NHLBI; DNA; Re; sequencing; Genotyping

PRINCIPAL INVESTIGATOR: Dr. Samuel Levy SUMMARY OF OBJECTIVES: The Center for Advancement in Genomics (TCAG) proposes a state of the art approach to resequence genes and genomic regions based on the application of highly advanced ONA sequencing technology and novel informatics tools. This program will be performed by a highly skilled team from the TCAG, The Institute for Genomic Research (TIGR) and the Joint Technology Center (JTC), Starting with genes and ONA samples selected by the NHLBI, we will optimize the polymerase chain reaction (PCR) to amplify ONA regions 800 bp in length for subsequent capillary based dye-terminator sequencing, Our scientific strategy, aimed toward achieving highly accurate ONA sequencing, uses a tiling strategy that assures two-fold coverage of each nucleotide, and facilitates the use of software for automated polymorphism calls using signal processing approach to sequence traces. In instances where this initial sequencing does not achieve the desired accuracy, additional sequencing reactions will be performed to assure the required quality.

首席执行官：Samuel Levy博士 证据摘要：基因组学进步中心（Center for Advancement in Genomics，TCAG）提出了一种基于高度先进的ONA测序技术和新型信息学工具的应用对基因和基因组区域进行重测序的最新方法。该计划将由来自TCAG，基因组研究所（TIGR）和联合技术中心（JTC）的高技能团队执行，从NHLBI选择的基因和ONA样本开始，我们将优化聚合酶链反应（PCR）以扩增长度为800 bp的ONA区域，用于随后的基于毛细管的染料终止子测序，我们的科学策略，旨在实现高度准确的ONA测序，使用平铺策略，确保每个核苷酸的两倍覆盖，并促进使用软件进行自动多态性调用，使用信号处理方法进行序列跟踪。在该初始测序未达到所需准确度的情况下，将进行额外的测序反应以确保所需的质量。