Nanophotonics of RE/DNA/nanotube materials: From bio-sensing-on-chip to live stem cell measurements

RE/DNA/纳米管材料的纳米光子学：从片上生物传感到活干细胞测量

• 批准号: 1822736
• 负责人: Slava Rotkin
• 金额: $ 10.95万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1822736&HistoricalAwards=false
• 关键词: Nanophotonics; RE; DNA; nanotube; materials

Abstract Title: Fundamental physics and biosensing applications of composite fluorescent nanomaterials rare-earths combined with DNA-enclosed carbon nanotubesNontechnical:Nanomaterials have influenced drastically several aspects of modern life, one of those is associated with their power in medicine and health care. This research involves rare-earth based materials due to their excellent fluorescence rare earths have already found a significant application niche in lighting, solar cells, sensors, biological and medical diagnostics and imaging. The bioimaging applications will be further advanced in this project. It is a continuation of the previous study in which rare-earths were combined with a unique nanocarbon material, so called single-wall nanotubes. Nanotubes by themselves has been already used in various miniaturized sensors, which are so extremely small that can be placed on a fabric thread. Most recently this group proposed using nanotubes enclosed in the DNA for studying live cells and has obtained promising results. It has been already demonstrated how nanomaterials could improve sensing capabilities of rare-earths. New project will extend this study and focus on nanotubes as tiny antennas to magnify the optical signals received by rare-earths. Due to small (nanometer) scale of these optical elements they can penetrate inside the cells and transmit the biologically relevant information from there, potentially improving our knowledge about how cells work as building blocks of organisms and helping medicine and health sciences in solving their current problems. This research is accompanied by a vigorous outreach program, aimed both on the local community and STEM students nationwide.Technical:This project focuses on developing and studying materials for bioimaging based on the rare-earth complexes with single-wall nanotubes and single strand DNA. Significance of this research is in developing a new biosensing material. Since single-wall nanotubes have diameters of only a few nanometers, they are comparable to many biological macromolecules such as enzymes, antibodies, DNA plasmids, etc. and may interact with intracellular environment. This makes them increasingly relevant for new opportunities in biomedical research and applications. Though at early stage, this project enlighten us about how the living organisms, at the cell level, may be influenced by new modern nanomaterials (nanotubes) upon ingestion. Using optical non-destructing characterization the fate of the nanotubes inside the cells has been already determined on the previous stage of the project. In the course of new project the team will work on intertwined theoretical and experimental tasks, including experimental optical characterization and theory of near-field electromagnetic modes in the hybrid materials of nanotubes-DNA-rare-earth-ions; exploring performance of these nanomaterials for bio-sensing applications in photonic-crystals and inside bio-mimetic hydrogels; studying the role of hotspots in nanotube materials and their influence on the photoluminescence of rare-earth ions and/or DNA; experimental studies of modulation of optical response of photonic crystals incorporating biosensing materials inside its lattice; application of these nanomaterials inside micro-fluidics sensing system and to studying in vitro neural stem cell. A series of samples will be studied, prepared at Lehigh University as well as at University of Utah, LANL and NIST via the collaboration links. A diverse program for dissemination of those results involves research advising and teaching at Lehigh, outreach at local schools (Palisades High School, Moravian Academy), participation in exhibition at a school children development National facility (the DaVinci Discovery Center), STEM enhancement programs via local Community Colleges (NCCC, LCTI and LCCC) and local Kutztown University, REU and GAAN programs within the Physics Department.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

摘要标题：复合荧光纳米材料的基本物理和生物传感应用稀土与DNA封闭的碳纳米管nontechnical：纳米材料对现代生活的几个方面产生了巨大影响，其中之一与其在医学和医疗保健中的能力相关。这项研究涉及基于稀土的材料，因为它们出色的荧光稀土已经发现了在照明，太阳能电池，传感器，生物学和医学诊断和成像中的重要应用。在该项目中，生物成像应用程序将进一步提高。这是先前的研究的延续，其中稀土与独特的纳米碳材料（所谓的单壁纳米管）结合在一起。纳米管本身已经被用于各种微型传感器中，这些传感器非常小，可以放在织物线上。最近，该组提出了使用DNA中包含在DNA中的纳米管来研究活细胞的，并获得了有希望的结果。它已经证明了纳米材料如何改善稀土的感应能力。新项目将扩展这项研究，并将重点放在纳米管上，作为微小的天线，以放大稀有物的光学信号。由于这些光学元件的小（纳米）量表很小（纳米），它们可以穿透细胞内部并从那里传输与生物学相关的信息，从而有可能提高我们对细胞如何作为生物体的构建和帮助医学和健康科学解决其当前问题的知识。这项研究伴随着一项激烈的宣传计划，既旨在探讨当地社区，又针对全国的STEM学生。技术：该项目着重于开发和研究基于带有单壁纳米管和单链DNA的稀有地球络合物的生物成像的材料。这项研究的意义在于开发一种新的生物传感材料。由于单壁纳米管的直径仅为少数纳米，因此它们与许多生物大分子相媲美，例如酶，抗体，DNA质粒等，并且可能与细胞内环境相互作用。这使它们与生物医学研究和应用的新机会越来越重要。尽管在早期，该项目启发了我们关于细胞水平的生物体如何在摄入后如何受到新现代纳米材料（纳米管）的影响。使用光学非破坏表征，已经在项目的上一个阶段确定了细胞内部纳米管的命运。在新项目的过程中，该团队将致力于交织的理论和实验任务，包括实验性光学特征和近场电磁模式的理论，纳米管 - 核心 - 雷亚尔 - 雷斯 - 近地材料的混合材料；探索这些纳米材料在光子晶体中和生物仿真水凝胶中的生物敏感性应用的性能；研究热点在纳米管材料中的作用及其对稀土离子和/或DNA的光致发光的影响；光子晶体的光学响应调节的实验研究，该光子晶体在其晶格中掺入生物传感材料；这些纳米材料在微流体传感系统中的应用和研究体外神经干细胞。将研究一系列样本，在Lehigh University以及犹他大学，Lanl和NIST通过协作链接准备。一项分散这些结果的多元化计划涉及在利哈伊（Lehigh），当地学校（摩拉维亚学院帕利塞德（Palisades）高中）举办的研究，在学校儿童发展国家设施（Davinci Discovery Center）参加展览会，通过当地社区学院（NCCC，LCTI和LCCC）的理由和kutztown Insport，Reus和Reu kib the STEM增强计划，REU和REUS TORPLICT INFORCTION，RESIS INCORDICT RECULTION，RESIS。法定任务，并被认为是值得通过基金会的智力优点和更广泛影响的审查标准来评估的值得支持的。