Re-Examining Southern California Lake Sediment Cores with Environmental DNA to Revolutionize How We Study the Past

用环境 DNA 重新审视南加州湖沉积物核心，彻底改变我们研究过去的方式

• 批准号: 1759756
• 负责人: Rachel Meyer
• 金额: $ 20.7万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-06-01 至 2021-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1759756&HistoricalAwards=false
• 关键词: Re; Examining; Southern; California; Lake

NATIONAL SCIENCE FOUNDATIONGEOGRAPHY SPATIAL SCIENCES (GSS) PROGRAMABSTRACT1759756Wayne, RobertUniversity of California-Los Angeles (No subs)This research project will investigate the human-environment relationship from the late Pleistocene era (approximately 120,000 BP) to the present in order to provide new understanding of the environmental dynamics prior to the arrival of humans in North America. The investigators will use environmental DNA (eDNA) to provide new insights for addressing long-standing questions of past human-environment relationships and biodiversity dynamics resulting from environmental change, fire, and human activities. The project will engage Native American communities in order to shape the procedures ancient eDNA studies take with regard to obtaining information about their ancestral activities. The investigators will build partnerships through this Native American network to initiate eDNA citizen science projects and virtual eDNA learning content. Through these activities, the investigators will offer educational and research opportunities to these underrepresented groups.Environmental DNA (eDNA) is a new methodology used for biodiversity monitoring. DNA shed by plants, animals, and other organisms is sequenced and evaluated, thereby allowing scientists to inventory the community at a location. Previous studies using eDNA on paleosoils and sediments have only been done in colder climates. This project will develop eDNA methods suitable for inventorying paleosediments in warmer climates by focusing on southern California, an area with a diverse history of environmental and landscape change over the timespan characterized by continuous human presence. The investigators will recover DNA shed by plants, animals, and microbes from well-dated, environmentally sensitive lake sediment cores, and they will use these data in combination with other paleoecological proxies. Using DNA techniques involving metabarcoding and hybridization, the investigators will inventory the flora and fauna of the past at low and high elevations levels and with different temperatures. Outcomes of this research will provide the scientific community and the public with the appropriate mechanisms to interpret ancient eDNA results.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

美国国家科学基金会地理空间科学项目（GSS）项目摘要：1759756 robert wayne，加州大学洛杉矶分校（No subs）本研究项目旨在研究更新世晚期（约120000 BP）至今的人类与环境的关系，从而对人类到达北美之前的环境动态提供新的认识。研究人员将利用环境DNA （eDNA）为解决过去人类与环境关系和生物多样性动态的长期问题提供新的见解，这些问题是由环境变化、火灾和人类活动引起的。该项目将吸引美洲土著社区参与，以形成古代dna研究在获取有关其祖先活动的信息方面所采取的程序。研究人员将通过这个美洲原住民网络建立伙伴关系，启动eDNA公民科学项目和虚拟eDNA学习内容。通过这些活动，调查人员将为这些代表性不足的群体提供教育和研究机会。环境DNA （Environmental DNA, eDNA）是一种用于生物多样性监测的新方法。通过对植物、动物和其他生物体脱落的DNA进行测序和评估，从而使科学家能够在一个地点清点群落。以前在古土壤和沉积物上使用eDNA的研究只在较冷的气候下进行。该项目将开发eDNA方法，适用于在温暖气候下的古沉积物，重点是南加州，一个在人类持续存在的时间跨度内具有多种环境和景观变化历史的地区。研究人员将从年代久远、环境敏感的湖泊沉积物岩心中恢复植物、动物和微生物脱落的DNA，并将这些数据与其他古生态指标结合使用。利用包括元条形码和杂交在内的DNA技术，研究人员将清点过去在低海拔和高海拔以及不同温度下的动植物。这项研究的结果将为科学界和公众提供解释古代eDNA结果的适当机制。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。