Digital probabilistic MRI atlas of primate brain with toolkits for surgery guidan

描述（由申请人提供）：该项目的目标是开发一个完全数字化的软件包，包括三维（3D）概率图谱、大脑和头部坐标系统以及用于侵入性手术指导和数据分析的工具包。将制作猕猴（Macaca mulatta）图谱。在灵长类动物脑的神经生物学研究中，神经酶起着重要的作用。与传统图谱相比，所提出的3D数字概率MRI图谱提供了（1）准确的体内解剖框架，而没有组织学载玻片带来的扭曲或收缩，（2）具有任意方向的2D切片，（3）便于数据映射的数字格式，（4）更具代表性的概率数据，以及（5）用于手术指导和数据分析的工具包。将以皮质表面几何形状和白色纤维束成像为标准检查标记的准确性。这个数字包将免费分发，以促进灵长类动物的神经生物学研究。扩散张量磁共振显微成像将用于建立具有高分辨率和高对比度的MRI图谱。高对比度和高分辨率分别来自扩散张量成像和显微成像。基于数字地图集，将开发实验前手术指导工具包和实验后数据分析工具包，并将其纳入一个用户友好的平台。技术和数据库的开发将通过四个目标集中进行。首先，将获得高质量的离体和体内数据，并将配准离体-体内数据。其次，标记和分割大部分灰质和白色物质神经结构。第三，通过将脑图像转换为模板来建立概率图谱和标记。第四，将开发一个用于指导侵入性操作和分析实验数据的平台。概率地图集是该提案的关键和核心部分。它在数字包中提供数据库。建立脑、头坐标系，规范构位关系系统。这两个工具包提供了地图集和坐标系统，为灵长类研究者使用地图集提供了方便实用的手段。 公共卫生相关性：提出的灵长类动物三维数字概率图谱将提供有关灵长类动物大脑结构的准确，全面和不断发展的数字帐户。该工具包的开发将为灵长类动物脑功能的研究带来精确、方便和高效的结果。灵长类动物脑与人类脑的密切关系也将极大地加深对人类脑功能的认识。

DESCRIPTION (provided by applicant): The goal of the project is to develop a fully digital package including three dimensional (3D) probabilistic atlas, brain and head coordinate systems and toolkits for invasive procedure guidance and data analysis. Atlas of macaque (Macaca mulatta) will be made. Atlases have been playing central roles for neurobiological studies on primate brains. Compared to conventional atlas, the proposed 3D digital probabilistic MRI atlas provides (1) accurate in vivo anatomical frame without distortion or shrinkage carried by histological slide, (2) 2D slice with arbitrary orientation, (3) digital format to facilitate data mapping, (4) probabilistic data to be more representative and (5) toolkits for surgery guidance and data analysis. The accuracy of labeling will be checked with cortical surface geometry and white matter fiber tractography as criteria. This digital package will be freely distributed to facilitate the neurobiological studies on primates. Diffusion tensor magnetic resonance microimaging will be used to set up the MRI atlas with high resolution and high contrasts. High contrasts and high resolution stem from diffusion tensor imaging and microimaging, respectively. Based on the digital atlas, pre-experiment surgery guidance toolkit and post-experiment data analysis toolkit will be developed and incorporated into a single user-friendly platform. Development of technologies and databases will be focused on through the four aims. First, high quality ex vivo and in vivo data will be acquired and the ex-in vivo data will be registered. Second, most of the gray and white matter neural structures will be labeled and segmented. Third, probabilistic atlas and labeling will be established by transforming the brain images to a template. Fourth, a platform for guidance of invasive procedure and analysis of the experimental data will be developed. The probabilistic atlas is the key and central part of the proposal. It provides the database in the digital package. Brain and head coordinates will be built up to standardize structure-location relationship system. With the atlas and coordinate systems, two toolkits provide the convenient and practical means for primate researchers to use the atlas. PUBLIC HEALTH RELEVANCE: The proposed three-dimensional digital probabilistic atlas of primates will provide an accurate, comprehensive and evolving digital account about the brain structure of primates. The toolkits developed to guide the surgery procedure and map the experimental data will bring precision, convenience and efficiency to facilitate the studies of primate brain function. And the understanding of human brain function will also be greatly enhanced because of the close relationship between primate brain and that of human.