Digital probabilistic MRI atlas of primate brain with toolkits for surgery guidan

描述（由申请人提供）：该项目的目标是开发一个全数字包，包括三维（3D）概率图谱，大脑和头部坐标系统以及用于侵入性手术指导和数据分析的工具包。制作猕猴（Macaca mulatta）图谱。地图集一直在灵长类动物大脑的神经生物学研究中发挥着核心作用。与传统的MRI图谱相比，本文提出的三维数字概率MRI图谱提供了(1)准确的体内解剖框架，没有组织切片带来的扭曲或收缩，(2)二维切片具有任意方向，(3)数字化格式便于数据映射，(4)概率数据更具代表性，(5)手术指导和数据分析的工具包。标记的准确性将以皮质表面几何形状和白质纤维束图作为标准进行检查。这个数字包将免费分发，以促进灵长类动物的神经生物学研究。利用扩散张量磁共振微成像技术建立高分辨率、高对比度的MRI图谱。高对比度和高分辨率分别源于扩散张量成像和微成像。基于数字图谱，将开发实验前手术指导工具包和实验后数据分析工具包，并将其整合到一个单一的用户友好平台中。技术和数据库的发展将通过这四个目标得到重点关注。首先，获得高质量的离体和体内数据，并对离体数据进行注册。其次，对大部分灰质和白质神经结构进行标记和分割。第三，将脑图像转化为模板，建立概率图谱和标记。第四，建立侵入性手术指导和实验数据分析平台。概率地图集是建议的关键和中心部分。它以数字包的形式提供数据库。建立大脑和头部坐标，规范结构-位置关系系统。这两个工具包结合了地图集和坐标系，为灵长类动物研究人员使用地图集提供了方便和实用的手段。

DESCRIPTION (provided by applicant): The goal of the project is to develop a fully digital package including three dimensional (3D) probabilistic atlas, brain and head coordinate systems and toolkits for invasive procedure guidance and data analysis. Atlas of macaque (Macaca mulatta) will be made. Atlases have been playing central roles for neurobiological studies on primate brains. Compared to conventional atlas, the proposed 3D digital probabilistic MRI atlas provides (1) accurate in vivo anatomical frame without distortion or shrinkage carried by histological slide, (2) 2D slice with arbitrary orientation, (3) digital format to facilitate data mapping, (4) probabilistic data to be more representative and (5) toolkits for surgery guidance and data analysis. The accuracy of labeling will be checked with cortical surface geometry and white matter fiber tractography as criteria. This digital package will be freely distributed to facilitate the neurobiological studies on primates. Diffusion tensor magnetic resonance microimaging will be used to set up the MRI atlas with high resolution and high contrasts. High contrasts and high resolution stem from diffusion tensor imaging and microimaging, respectively. Based on the digital atlas, pre-experiment surgery guidance toolkit and post-experiment data analysis toolkit will be developed and incorporated into a single user-friendly platform. Development of technologies and databases will be focused on through the four aims. First, high quality ex vivo and in vivo data will be acquired and the ex-in vivo data will be registered. Second, most of the gray and white matter neural structures will be labeled and segmented. Third, probabilistic atlas and labeling will be established by transforming the brain images to a template. Fourth, a platform for guidance of invasive procedure and analysis of the experimental data will be developed. The probabilistic atlas is the key and central part of the proposal. It provides the database in the digital package. Brain and head coordinates will be built up to standardize structure-location relationship system. With the atlas and coordinate systems, two toolkits provide the convenient and practical means for primate researchers to use the atlas. PUBLIC HEALTH RELEVANCE: The proposed three-dimensional digital probabilistic atlas of primates will provide an accurate, comprehensive and evolving digital account about the brain structure of primates. The toolkits developed to guide the surgery procedure and map the experimental data will bring precision, convenience and efficiency to facilitate the studies of primate brain function. And the understanding of human brain function will also be greatly enhanced because of the close relationship between primate brain and that of human.