The Rabbit Genome, Immune System and Ig Genetics

兔是几种传染病的唯一或最佳动物模型。兔子基因组的序列目前尚未完成2倍，但更深的7倍覆盖范围始于2007年。2006年NHGRI建议对兔子进行更深入的测序，将NIAID等位基因定义的兔子描述为未来SNP发现的宝贵资源。它们具有多种免疫系统基因的多态，包括抗体分子的VH、CH和CL区的等位基因异型。该克隆还包括以前在巴塞尔免疫学研究所工作的兔子的后代，包括VH1a2缺失的Alicia、CK1剪接缺陷Basilea的突变体和野生型亲本，以及几种VH-CH重组重链类型。这些兔子被提供给感兴趣的个人，特别是可以建立繁殖群体的地点。一个4D关系数据库包含超过45年的繁殖记录和有关该群体中动物的其他信息。突变的ALI动物存在免疫球蛋白重链基因的关键可变区基因缺失，该基因存在于相关的2R1野生型兔中。这些突变体可能更容易感染传染病，因为它们的体液免疫发育异常迟缓。作为我在2005年与人合著的一份白皮书建议对兔子基因组进行深度测序的部分结果，博德研究所、麻省理工学院和哈佛大学于2008年7月完成了7x测序。预计将在2009年初召开深度报道大会。我们能够在不完整的2x轨迹档案中找到并使用的序列，以及在7x轨迹档案中的序列，已经证明对各种研究有用。由于我的努力，现在有两个网站可以使用，一个由国家生物技术信息中心(NCBI)http://www.ncbi.nlm.nih.gov/projects/genome/guide/rabbit/维护，另一个由美国国家免疫与传染病研究所(NIAID)在免疫学和传染病(http://www3.niaid.nih.gov/research/resources/ri/.上维护后一个网站提供了NIAID在2005年举办的关于人类传染病的兔子模型的研讨会的摘要，我帮助外部NIAID DMID规划和主持了该研讨会。

Rabbits are the only or best animal model for several infectious diseases. The sequence of the rabbit genome is currently unfinished at 2x, but deeper 7x coverage started in 2007. A 2006 NHGRI recommendation that rabbit be sequenced more deeply, describes the NIAID allotype-defined rabbits as a valuable resource for future SNP discovery. They have polymorphisms of a variety of immune system genes including allelic allotypes of the VH, CH, and CL regions of antibody molecules. The colony also contained descendants of rabbits formerly at the Basel Institute for Immunology, including mutant and wild-type parental of VH1a2-deleted Alicia, CK1 splicing defective Basilea, and several VH-CH recombinant heavy chain types. These rabbits were made available to interested individuals, particularly to sites where breeding colonies could be established. A 4D relational database contains more than 45 years of breeding records and other information about animals in the colony. The mutant ali animals have a deletion of a key variable region gene in the immunoglobulin heavy chain locus that is present in related 2R1 wild type rabbits. The mutants may be more susceptibe to infectious disease because they have abnormal delayed development of humoral immunity. In part as the result of a White Paper I coauthored in 2005 proposing deep sequencing of the rabbit genome, 7x coverage was completed In July 2008 at Broad Institute MIT and Harvard. Assembly of the deep coverage is anticipated in early 2009. The sequences we have been able to find and use in the incomplete 2x trace archive, and in the 7x trace archive have already proven useful for various studies. As a result of my efforts, two websites are now available one maintained by the National Center for Biotechnology Information (NCBI) http://www.ncbi.nlm.nih.gov/projects/genome/guide/rabbit/ -- and one by NIAID on Rabbit in Immunology & Infectious Disease (http://www3.niaid.nih.gov/research/resources/ri/. The latter site offers a summary of an NIAID workshop on Rabbit Models of Human Infectious diseases held in 2005 that I helped the extramural NIAID DMID to program and chair.