Transgenic Core - Greengard (pgs. 218 - 235)

抗精神病药物的分子靶点及其相关的细胞内信号转导研究 通路已经产生了大量关于与精神分裂症相关的细胞扰动的信息。这个 项目1-5中概述的综合研究将在现有知识的基础上与严谨的生物学、 抗精神病药物治疗中神经细胞的分子、生化、行为和电生理研究 这些研究有可能为精神分裂症/精神分裂症的病因学提供更多的洞察力，并使 可能的新的和/或更有效的抗精神病药物治疗。 Transgcnic Animal Core将是一个致力于促进泰国项目1-5中提出的实验的中心 需要使用转基因动物。这些项目中概述的实验需要创造和 维护大量转基因动物；生产这些转基因动物将由Tie Core负责 以高效、及时和经济高效的方式进行转基因、基因敲除和敲打动物。存在着一种 配备有经验的人员的中央设施将确保这是可行的。为了生产奢华的 转基因动物的数量高效，核心的具体目标I将是生产携带BAC的 转基因。然后，核心将在特定的目标II中使用这些经过修改的BAC，生产转基因和 击倒小鼠的线条和这些线条的测试。在产生转基因和条件基因敲除时 动物，核心将测试和表征这些动物，以验证它们是否可以在拟议的研究中使用，L说。 核心的具体目标三将是培育和维护这些和其他小鼠品系(这将是 在项目1-5的整个研究中使用。

Investigations into the molecular targets of neuroleptic drugs and their associated intracellular signaling pathways have yielded a wealth of information regardingthe cellular perturbations associatedwith schi/ophrenia. The combined studies outlined in Projects 1-5 will extend upon this existing knowledge with rigorouscell biological, molecular, biochemical,behavioral, and electrophysiological studies of neuronal cells upon neuroleptic drug treatment These studies have the potential to provide greater insights into the etiology of schi/ophrenia as well as lo make possible novel and/or more efficient neuroleptic drug treatment. The Transgcnic Animal Core will be a center devoted to facilitating those experiments proposed in Projects 1-5 thai require the use of genetically modified animals.The experimentsoutlined in these Projects require the creation and maintenance of large numbersof genetically modified animals; it will be the responsibility of tie Core to produce these transgenic, knockout, and knockin animalsin an efficient, timely, and cost-effective manner. The existence of a centralized facility staffed with experienced personnel will ensure that this is feasible. In order to produce lurge numbers of transgenic animalsefficiently, Specific Aim I of the Core will be the production of BACs carrying transgenes. The Core will then use these modified BACs in Specific Aim II, the production of transgenic and knockout mouse lines and the testing of these lines. Upon production of transgenic and conditional knockout animals, the Core will test and characterize these animals to validatethem for use in the proposed studies, l-'inally, Specific Aim III of the Core will be the breeding and maintenance of these and other mouse lines (hat will be used throughout the studies in Projects 1-5.