MOLECULAR ARCHITECTURE OF NUCLEAR PORE COMPLEX (NPC) FROM YEAST S CEREVISIAE

酿酒酵母核孔复合物 (NPC) 的分子结构

• 批准号: 7722187
• 负责人: MICHAEL P ROUT
• 金额: $ 4.43万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722187
• 关键词: Architecture; Coated vesicle; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Disease; Employee Strikes; Face; Funding; Grant; Institution; Localized; Membrane; Methods; Molecular; Nuclear Envelope; Nuclear Pore Complex; Paper; Proteins; Proteomics; Research; Research Personnel; Resources; Source; Structure; Surface; United States National Institutes of Health; Yeasts; numb protein; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nuclear pore complexes (NPCs) are proteinaceous assemblies of approximately 50 MDa that selectively transport cargoes across the nuclear envelope. To determine the molecular architecture of the yeast NPC, we collected a diverse set of biophysical and proteomic data, and developed a method for using these data to localize the NPC's 456 constituent proteins (see the accompanying paper). Our structure reveals that half of the NPC is made up of a core scaffold, which is structurally analogous to vesicle-coating complexes. This scaffold forms an interlaced network that coats the entire curved surface of the nuclear envelope membrane within which the NPC is embedded. The selective barrier for transport is formed by large numbers of proteins with disordered regions that line the inner face of the scaffold. The NPC consists of only a few structural modules that resemble each other in terms of the configuration of their homologous constituents, the most striking of these being a 16-fold repetition of 'columns'. These findings provide clues to the evolutionary origins of the NPC.

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