Equipment Supplement for the National Center for Dynamic Interactome Research

国家动态相互作用组研究中心的设备补充

• 批准号: 10392609
• 负责人: MICHAEL P ROUT
• 金额: $ 24.56万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10392609
• 关键词: Address; Area; Automobile Driving; Binding; Biological; Biological Process; Cell Shape; Cells; Cellular biology; Collaborations; Communities; Complex; Couples; Data; Development; Disease; Equipment; Explosion; Future; Genomics; Goals; Intervention; Laboratories; Life; Mass Spectrum Analysis; Mediating; Medical Research; Modeling; Molecular; Nucleic Acids; Pathogenicity; Pathology; Pharmacologic Substance; Property; Proteins; Research; Research Personnel; Structure; Systems Biology; Technology; Testing; Time; Training; Viral Cancer; Virus Diseases; base; biological systems; cell behavior; design; experimental study; genomic data; improved; insight; macromolecule; predictive modeling; preservation; program dissemination; structural biology; tool

PROJECT SUMMARY Overall: The National Center for Dynamic Interactome Research. The emergent properties of life require the dynamic interactions of macromolecules, the two major classes of which are proteins and nucleic acids. These interactions form macromolecular machines and dynamic liaisons that shape the cell, transmit information and control cellular behaviors, and pathogenic alterations in molecular interaction networks (interactomes) underlie disease. Our understanding and modulation of biological systems, as well as their pathologies, thus relies on the ability to elucidate and interpret these interactions and their dynamics. For nucleic acids, recent advances have led to an explosion of genomic data. However, proteins are incredibly diverse in their abundance and their properties, making them highly versatile for their dynamic tasks, but at the same time exceptionally difficult to analyze. It is for these reasons that the interactomic revolution still lags very far behind the genomic revolution. The National Center for Dynamic Interactome Research (NCDIR) couples cell biology laboratories, a mass spectrometry laboratory, a systems biology laboratory, and a computational structural biology laboratory. The goal of the NCDIR is to synergistically pioneer new and improved approaches, integrating these technologies into a fundamentally unique “pipeline” approach to address the urgent need of the biomedical community for technologies that can rapidly, reliably and routinely identify and characterize the dynamic cellular interactome. Our pipeline approach begins by developing technologies for purifying and preserving, with high fidelity, various defined forms of the hierarchical arrangement of interactors surrounding any chosen macromolecule. We then provide comprehensive, highly quantitative, detailed temporal and structural data for dynamic complexes. Such data is used to generate structural and mechanistic models that are predictive, testable, actionable, and guide experiments to focus on those that are most informative. The models aim to provide the biomedical community with the means for rational target-based future experimentation, functional characterizations and even intervention. These approaches will be developed, refined and beta-tested via a selected set of Driving Biological Projects and Collaborations that can enter and exit our pipeline at any point, and which present specific technological challenges. Critical to the design of the NCDIR is an effective training and dissemination program that is responsive to the urgent needs of the biomedical community.

项目总结