D1 AND D5 RECEPTOR SIGNALING IN MONKEY PFC

点击翻译按钮获取中文摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to use electron microscopic techniques to determine the localization of the D1 and D5 dopamine receptors and their signaling proteins within primate prefrontal cortex, and to identify any physical interactions between these receptors and their downstream effector proteins utilizing immunoprecipitation methods. The prefrontal cortex has a complicated circuitry, and understanding where these two receptors are located within specific elements of this circuitry will deepen our understanding of how D1 family receptor signaling effects prefrontal functioning. We have determined the localization of D1 and D5 in prefrontal cortex (PFC) neuropil of layers I, III and V; determined their co-localization within dendritic spines and axon terminals of layer III; and determined D1 and D5 localization to parvalbumin and calretinin interneuron dendrites and axon terminals. During the reporting period, we determined the neuropil localization of two signaling proteins, DARPP-32 and Inhibitor-1, in layer III of the primate PFC; and determined the localization of protein phosphatase-1 isoforms to parvalbumin interneurons. Moreover, we made significant progress in determining the localization of protein phosphatase-1 isoforms to calretinin interneurons.