Temporal Regulation of miR156

miR156 的时间调控

• 批准号: 7609183
• 负责人: Keith William Earley
• 金额: $ 4.72万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7609183
• 关键词: Adolescent; Adult; Alleles; Arabidopsis; Binding; Biogenesis; Biological; Cell Cycle; Cell Death; Cell Differentiation process; Code; Collection; Complementary RNA; Complex; Computer Simulation; Development; Developmental Biology; Developmental Process; Disease; Elements; Enhancers; Family; Flowers; Gene Expression; Gene Expression Regulation; Gene Transfer; Generations; Genes; Genetic; Human; Left; Link; Maize; Malignant Neoplasms; Mammals; Mediating; MicroRNAs; Mouse-ear Cress; Mutation; Northern Blotting; Organism; Orthologous Gene; Pathway interactions; Pattern; Phenotype; Plant Leaves; Plants; Play; Process; Proteins; RNA Binding; RNA Sequences; Regulation; Reporter; Research Training; Reverse Transcriptase Polymerase Chain Reaction; Role; Screening procedure; Staging; System; Testing; Time; Tissues; Transcript; Virus Diseases; fly; insulin secretion; member; mutant; phase change; plant growth/development; promoter; reproductive; research study; tool; transcription factor

DESCRIPTION (provided by applicant): MicroRNAs (miRNAs) are short, non-protein coding RNAs that bind to near complementary RNA sequences to inhibit gene expression. The control of gene expression by miRNAs has emerged as an important mechanism for gene regulation in a wide range of organisms (flies, worms, plants, and mammals) as well as a potential tool for treating disease. MiRNAs have recently been demonstrated to play an important role in the regulation of pathological conditions in humans related to cell death, viral infection, insulin secretion and cancer. Furthermore, it is estimated that over 30% of all human genes are regulated by miRNAs. MiRNAs regulate many aspects of plant development. Among these is the process of developmental phase change. Early in their development, Arabidopsis plants produce morphologically simple juvenile leaves. Later, they undergo a transition known as vegetative phase change and begin to produce more complex adult leaves. Finally, the plants produce flowers, marking the final stage of plant development. The miRNA, miR156, is a major regulator of this process. Using the experiments described in this proposal, I will define the factors that contribute to the temporal expression of miR156. Specifically, I will: AIM 1: Determine if miR156a temporally regulates SPL expression using mutant alleles of the miR156 and its targets. AIM 2: Develop reporters for use in promoter-deletion studies and mutant screens to identify cis- and transfactors involved in the temporal regulation of miR156a. AIMS: Identify genes that regulate miR156 expression by screening for suppressors and enhancers of sqn. The results of these studies will not only contribute to the understanding of leaf development, but will also increase our understanding of the mechanism of temporal regulation, a key process in developmental biology of all organisms.

描述（由申请人提供）：MicroRNAs （miRNAs）是一种短的非蛋白质编码RNA，可结合近互补RNA序列抑制基因表达。mirna对基因表达的控制已成为多种生物（苍蝇、蠕虫、植物和哺乳动物）基因调控的重要机制，也是治疗疾病的潜在工具。mirna最近被证明在调节与细胞死亡、病毒感染、胰岛素分泌和癌症相关的人类病理状况中发挥重要作用。此外，据估计，超过30%的人类基因受mirna调控。mirna调控植物发育的许多方面。其中包括发育阶段变化的过程。在发育早期，拟南芥植物产生形态简单的幼叶。之后，它们经历一个被称为营养阶段变化的过渡，并开始产生更复杂的成叶。最后，植物开花，标志着植物发育的最后阶段。miRNA miR156是这一过程的主要调节因子。使用本提案中描述的实验，我将定义有助于miR156时间表达的因素。具体来说，我会：