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Cell and Virus CORE

Cell and Virus CORE
细胞和病毒核心
批准号：
7600770
负责人：
David J Rawlings
金额：
$17.5万
依托单位：
SEATTLE CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
项目状态：
已结题
起止时间：
2007-09-30 至 2012-06-30
来源：
https://reporter.nih.gov/project-details/7600770
关键词：
AffinityAnimalsAttentionAvidityB-LymphocytesBindingBiological AssayCalibrationCanis familiarisCapitalCell LineCell SeparationCellsCompatibleConfusionCore FacilityCultured CellsDNADevelopmentElementsEngineeringEngraftmentEnsureEquipmentFacility Construction Funding CategoryFamiliarityFiltrationFlow CytometryFrequenciesGene TargetingGene TransferGenerationsGenesGoalsHeadHematopoieticHematopoietic stem cellsHomingHourHumanHuman ResourcesImmuneImmune systemImmunoglobulin Somatic HypermutationImmunologyIndividualIntegraseJointsLaboratoriesLentivirus VectorLibrariesMGMT geneMagnetismMaintenanceMethodsModificationMusNaturePediatric HospitalsPopulationPreparationProductionPropertyProteinsRateReporterReportingResearchResearch PersonnelResourcesRiskScreening procedureSideSiteSorting - Cell MovementStandards of Weights and MeasuresStem cellsSubfamily lentivirinaeSucroseSupporting CellSurfaceSystemTechnologyTimeTransplantationUltracentrifugationVariantVertebral columnViralVirusWeekWorkZinc Fingersabstractingbasecell typecostdesignendonucleaseenhancing factorexperiencegene repairimmune functionin vivoinstrumentinstrumentationmagnetic cell separationnovelnucleaseparticlepromoterreconstitutionrepairedresearch studyvectorvirus core

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The Cell and Virus Core is designed to support two major activities of the NGEC: cell separations and analysis, and construction and production of self inactivating non-integrating lentiviral (NIL) vectors and viral stocks. AutoMACs and flow cytometry and sorting will be heavily utilized in Components 2-5 for LHE design, and in Components 6-8 for analysis of fluorescent reporter assays and immune function in in vivo gene targeting and repair experiments. NIL vectors will be used for delivery of LHE's and donor templates in selected experiments from Component 1, and in virtually all experiments proposed in Components 6-8.
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