Fundamental Cryobiology of Mammalian Embryonic Stem Cells

哺乳动物胚胎干细胞的基础低温生物学

• 批准号: 7662793
• 负责人: LELA K. RILEY
• 金额: $ 9.98万
• 依托单位: IMPACT BIOLABS, LLC
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-05 至 2011-06-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7662793
• 关键词: Address; American; Animals; Area; Biological Preservation; Biomedical Research; California; Canada; Cell Line; Cell Maintenance; Cell Volumes; Cell membrane; Cells; Characteristics; Cryopreservation; Cryoprotective Agents; Dependence; Devices; Dimethyl Sulfoxide; Disease; ES Cell Line; Embryonic Development; Environment; Ethylene Glycols; European; Excision; Explosion; Freezing; Genes; Genetic; Genomics; Genotype; Germ Lines; Glycerol; Goals; Hand; Human; Human Biology; Intellectual Property; Knock-out; Knockout Mice; Laboratories; Life; Macaca mulatta; Maintenance; Medicine; Methods; Modeling; Mus; Mutagenesis; Mutant Strains Mice; Nature; Permeability; Personal Communication; Pharmacology; Phase; Production; Propylene Glycols; Protocols documentation; Recovery; Regenerative Medicine; Research Personnel; Resources; Series; Technology; Temperature; Transgenic Mice; Transportation; Undifferentiated; Universities; Water; Work; base; cell type; cold temperature; comparative; cost; cryobiology; design; embryonic stem cell; ethylene glycol; genetic manipulation; human embryonic stem cell; improved; mathematical model; mouse model; mutant; novel; progesterone 11-hemisuccinate-(2-iodohistamine); programs; public health relevance; research study; restoration; stem cell technology; user-friendly; virtual

DESCRIPTION (provided by applicant): The overall goal of this project is to establish new and novel methods and materials that will improve the low temperature storage of mammalian embryonic stem cells (ESCs). Here we propose to work only with mouse (i.e. non-human) ESCs. This work will result in intellectual property for the production of devices and disposables (e.g., media). ESCs are important biomedical research resources due to their broad applications in the areas of comparative medicine, comparative genomics, pharmacology, embryogenesis, and regenerative medicine. To fully utilize the potential of ESCs, it is necessary to have reliable methods for ESC maintenance, transportation, and cryopreservation. However, current cryopreservation protocols for ESCs remain suboptimal and highly variable. In order to improve cryopreservation methods for ESCs of all species, it is essential to understand their fundamental cryobiology in order to efficiently predict optimized protocols that are easily incorporated into routine laboratory use for both small and large scale ESC preservation. We will conduct a series of experiments to address these issues currently restricting optimal utilization of ESC and develop commercializable methods and products (devices and disposables) to implement these new, optimal cryopreservation methods. PUBLIC HEALTH RELEVANCE: Embryonic stem cells (ESCs) are important because of their broad potential applications in human medicine. However, current cryopreservation methods for ESCs are suboptimal and highly variable. The goal of this project is to establish novel methods and devices that improve cryopreservation of ESCs.

描述（由申请人提供）：本项目的总体目标是建立新的和新颖的方法和材料，将改善哺乳动物胚胎干细胞（ESC）的低温储存。在这里，我们建议仅使用小鼠（即非人类）胚胎干细胞。这项工作将产生用于生产器械和一次性用品的知识产权（例如，媒体）。胚胎干细胞是重要的生物医学研究资源，因为它们在比较医学、比较基因组学、药理学、胚胎发生和再生医学等领域有着广泛的应用。为了充分发挥ESC的潜力，需要有可靠的ESC维护、运输和冷冻保存方法。然而，目前的胚胎干细胞冷冻保存方案仍然是次优的和高度可变的。为了改进所有物种胚胎干细胞的冷冻保存方法，有必要了解其基本的冷冻生物学，以便有效地预测易于纳入常规实验室用于小型和大型胚胎干细胞保存的优化方案。我们将进行一系列实验来解决目前限制ESC最佳利用的这些问题，并开发可商业化的方法和产品（设备和一次性用品）来实施这些新的最佳冷冻保存方法。公共卫生相关性：胚胎干细胞（ESC）非常重要，因为它们在人类医学中具有广泛的潜在应用。然而，目前用于ESC的冷冻保存方法是次优的并且高度可变。本项目的目标是建立新的方法和设备，提高胚胎干细胞的冷冻保存。