Highly Parallel AIDS Assays Using A Microfluidic Flow Cell Array Integrated with

项目描述（由申请人提供）：该项目的最终目标是开发一个无多重标签的平台，用于患者样本的高通量HIV特异性血清抗体检测。为了帮助Wasatch microfluics实现这一最终目标，本提案将通过应用一种新的微流控斑点技术到可用的表面等离子体共振（SPR）阵列平台，解决对各种HIV蛋白的血清抗体进行高通量无标签检测的可行性。具体来说，微流体流动细胞阵列将适用于“真实世界”样品，如血浆、血清和细胞裂解物。流式细胞阵列将有48个通道，并处理一系列点，旨在支持正在进行的非人类灵长类动物临床前试验以及人类艾滋病毒疫苗试验。开发一种低成本、高通量、多重检测方法来评估疫苗接种后体液免疫反应的发展，对于全面的免疫监测至关重要。公共卫生相关性：该提案的成功完成将大大提高在药物发现和蛋白质组学研究中进行多重基于spr的分析的能力。在更大的范围内，通过显著提高无标签筛选的吞吐量，这将导致改进诊断工具，减少药物开发的成本和时间。

DESCRIPTION (provided by applicant): The ultimate goal of this project is to develop a multiplex label free platform for high-throughput HIV specific serum antibody detection of patient samples. To help Wasatch Microfludics reach this ultimate goal, this proposal will address the feasibility of performing high throughput label free detection of serum antibodies to a variety of HIV proteins through the application of a novel microfluidic spotting technology to available Surface Plasmon Resonance (SPR) array platforms. Specifically, a microfluidic flow cell array will be adapted for work with "real world" samples such as plasma, serum, and cell lysates. The flow cell array will have 48 channels and address an array of spots designed to support ongoing pre- clinical trials in non-human primates as well as HIV vaccine trials in humans. The development of a low cost, high throughput, multiplex assay to assess the development of humoral immune responses following vaccine administration is essential for comprehensive immunomonitoring. PUBLIC HEALTH RELEVANCE: The successful completion of this proposal will create a dramatic improvement in the ability to conduct multiplex SPR-based assays in drug discovery and proteomic research. On a greater scale, this will lead to improved diagnostic tools and reductions in the cost and time of drug development, by significantly improving the throughput of label free screening.