Parallel Microfluidic System for High Throughput Label Free Cytokine Analysis

用于高通量无标记细胞因子分析的并行微流体系统

• 批准号: 7539032
• 负责人: Bruce Gale
• 金额: $ 19.98万
• 依托单位: CARTERRA, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-15 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7539032
• 关键词: Acute; Address; Arts; Basic Science; Binding; Biosensor; Cells; Chronic Disease; Communication; Cost Savings; Data; Deposition; Detection; Development; Discrimination; Equipment; Excision; Goals; Image; Immune response; Institutes; Kinetics; Label; Liquid substance; Location; Marketing; Measurement; Methods; Microfluidics; Microscopic; Modification; Monitor; Numbers; Patients; Performance; Phase; Printing; Process; Protein Analysis; Proteomics; Reaction; Reagent; Reproducibility; Research; Running; Sampling; Screening procedure; Spottings; Surface; Surface Plasmon Resonance; Suspension substance; Suspensions; System; Technology; Testing; Time; Validation; Work; base; chemokine; cost; cytokine; drug discovery; improved; instrument; novel; sensor; tool; trafficking; two-dimensional

DESCRIPTION (provided by applicant): The ultimate goal of this project is to develop a multiplex label free platform for high- throughput cytokine screening of patient samples. Though there are benefits to the current suspension phase multiplex cytokine analysis tools (Luminex), the technology suffers from a number of drawbacks including reagent variability, high reagent costs, and long run times. The development of a high throughput label free system will improve the throughput and reproducibility of cytokine analyses. In addition, cost savings will be realized through reductions in development costs and the removal of labeling reagents. Accordingly, Wasatch Microfluidics will team with the Baylor Institute of Immunological Research (BIIR) to adapt Wasatch's continuous flow spotting technologies to create a flow cell array directly integrated with a commercial biosensor array platform. Currently, flow cell technology is the limiting factor in the development of high throughput label free sensing technologies. Modification of Wasatch Microfluidics Continuous Flow MicrospotterTM into a highly parallel flow cell should begin to eliminate this bottleneck and provide a template for even more highly parallel systems. Preliminary work suggests that a flow cell array can convert mediocre SPR imaging instruments into highly competitive protein analysis instruments comparable to state-of-the-art SPR instruments with meager throughput. Once the flow cell is completed and integrated with the commercial biosensor platform at the BIIR, validation testing will be conducted to demonstrate cytokine discrimination and quantitative measurement of cytokine levels in a high throughput multiplex label free format.

描述(申请人提供)：该项目的最终目标是开发一个用于高通量患者样本细胞因子筛选的多重无标记平台。虽然目前的悬浮相多重细胞因子分析工具(Luminex)有好处，但该技术存在许多缺点，包括试剂可变性、试剂成本高和运行时间长。高通量无标记体系的发展将提高细胞因子分析的吞吐量和重复性。此外，还将通过降低开发成本和取消标签试剂来实现成本节约。因此，Wasatch Microfluidics将与贝勒免疫研究所(BIIR)合作，采用Wasatch的连续流动检测技术，创建直接与商业生物传感器阵列平台集成的流动细胞阵列。目前，流动细胞技术是高通量无标记传感技术发展的限制因素。将Wasatch Microfluidics Continued Flow MicrospecterTM改造成高度平行的流动池将开始消除这一瓶颈，并为更高度平行的系统提供模板。初步工作表明，流动细胞阵列可以将平庸的SPR成像仪器转变为具有极强竞争力的蛋白质分析仪器，可与吞吐量极低的最先进的SPR仪器相媲美。一旦流动池完成并与BIIR的商业生物传感器平台集成，将进行验证测试，以高通量多路无标记格式演示细胞因子识别和细胞因子水平的定量测量。