Tissue Management Shared Resource

组织管理共享资源

• 批准号: 8180824
• 负责人: JAMES K. WILLSON
• 金额: $ 9.71万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-03 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8180824
• 关键词: African American; Anatomy; Biocompatible Materials; Blood; Bone Marrow; Breast; Cancer Center Support Grant; Caucasians; Caucasoid Race; Cells; Clinical; Colon; Computer software; Consent; DNA; Data; Databases; Ensure; Female; Freezing; Growth; Hispanics; Histology; Human; Individual; Kidney; Laboratories; Lasers; Liquid substance; Lung; Malignant Neoplasms; Medical Records; Operative Surgical Procedures; Ovary; Paraffin; Participant; Pathologist; Pathology; Patient Participation; Prostate; Quality Control; RNA; Research; Research Ethics Committees; Resource Sharing; Resources; Sampling; Secure; Services; Specimen; Staining method; Stains; Tissue Procurements; Tissues; base; demographics; tissue culture; web interface

The Tissue Management Shared Resource provides IRB-approved centralized tissue procurement services for various specimens derived from consented human participants. Over 4500 tissue specimens and bodily fluids, from 52 different fissue types, especially blood, bone marrow, breast, colon, kidney, lung, ovary and prostate, and 84 defined cancer subtypes are available. In terms of demographics, 65%, 17% and 15% of specimens are derived from Caucasian, African-American and Hispanic participants, respectively; 59% of specimens are derived from female participants. All fissue specimens are evaluated by a board-certified anatomic pathologist for quality control purposes, ensuring that the specimens used for research are of the highest quality. For every specimen, extensive data is captured from the participant's medical record, including demographics, clinical findings, diagnosfic laboratory tesfing results, treatment information and pathology assessment results. These data are captured and stored in the UT Southwestern Biomaterial Tracking and Management (BTM) software application, which includes a relafional database that stores these data in a de-identified manner that can be accessed and searched through a secure web interface to authorized invesfigators. In addifion to procurement services, the resource offers a number of other ancillary services. DNA and RNA fractions are routinely isolated from procured specimens. Standard histology services - embedding (fresh and fixed), secfioning (frozen and fixed), and staining - are offered for all specimens. Laser capture microdissecfion of fissue secfions is offered for invesfigators wishing to isolate specific cells within a fissue sample for further analysis. Laser capture is offered at two levels of resolufion - capture of fissue regions and capture of individual cell sets - depending on the specific research needs of the invesfigator. The resource offers services for the construcfion of fissue microarray in which as many as 768 samples can be arrayed in a single paraffin block and sectioned for immunohistochemical analysis. The resource is equipped for the isolafion and growth of primary cells from pafient samples for tissue culture studies and xenotransplantafion. In order to ensure the highest level of compliance and consistency, resource staff routinely takes responsibility for consenting surgical patients for participation in fissue procurement-based research.

组织管理共享资源提供IRB批准的集中组织采购服务 从自愿的人类参与者身上提取的各种标本。超过4500个组织标本和身体 液体，从52个不同的裂隙类型，特别是血液，骨髓，乳房，结肠，肾脏，肺，卵巢和 前列腺和84种定义的癌症亚型。在人口统计方面，65%，17%和15%的 标本分别来自高加索人、非洲裔美国人和西班牙裔参与者; 59%的 样本来自女性参与者。所有的裂缝样本都由一个委员会认证的 解剖病理学家进行质量控制，确保用于研究的标本是 最高品质。对于每个样本，从参与者的医疗记录中获取大量数据， 包括人口统计学、临床发现、诊断实验室检测结果、治疗信息和 病理学评估结果。这些数据被捕获并存储在UT西南生物材料中 跟踪和管理（BTM）软件应用程序，其中包括一个相关数据库， 这些数据以去识别的方式，可以通过安全的Web界面访问和搜索， 授权投资者。除了采购服务外，该资源还提供了一些其他辅助服务， 服务DNA和RNA组分通常从采购的标本中分离出来。标准组织学 为所有人提供包埋（新鲜和固定）、切片（冷冻和固定）和染色服务。 标本激光捕获显微解剖的裂隙部分提供给研究者希望分离 用于进一步分析。激光捕获提供两种分辨率- 裂隙区域的捕获和单个细胞集的捕获-取决于特定的研究需求， 投资者该资源为构建裂缝微阵列提供服务， 768个样本可以排列在单个石蜡块中并切片用于免疫组织化学分析。的 为组织培养的原代细胞的分离和生长配备了资源 研究和异种移植。为了确保最高水平的合规性和一致性， 资源工作人员通常负责征得手术患者的同意，以便参与手术 基于采购的研究。