High Throughput Screening Shared Resources

高吞吐量筛查共享资源允许癌症中心的成员使用 化合物或基因组siRNA文库的高通量筛选技术。HTS 实验室提供目标评估和化验评估、化验开发和测试、筛选 化合物或siRNA库、数据分析和存储以及“命中”验证。获得访问权限 HTS设施，癌症中心成员必须有一个由癌症中心HTS评估的项目 监督委员会，该委员会将评估其实用性以及科学和治疗价值 筛查的目标。对于已获批准的项目，HTS工作人员随后将评估 建议化验，并与指定实验室合作，将化验开发为适合HTS的形式 可重复地达到所需的信噪比。HTS实验室致力于最大限度地降低 这项经常需要更换试剂和某些试剂的实验完全重新设计了分析方法。 在小规模实验中，一旦化验具有可接受的信噪比，对于复合筛查 多重试板用DMSO(复合稀释剂)处理，以测定重复性。 类似于HTS筛选的条件，然后对照测试化合物文库进行筛选，以确定 命中率。基因组siRNA实验是以类似的方式进行的，只是它们在96个井中运行 格式，并且在基因组筛选开始之前不使用测试库。就像在 化合物，siRNA实验对照必须在运行中实现对背景rafio的可重现信号 多个平板前的项目被允许筛选基因组文库。

The High Throughput Screeening Shared Resource allows members of the Cancer Center to use the techniques of high-throughput screening (HTS) of compounds or genomic siRNA libraries. The HTS laboratory provides target assessment and assay evaluafion, assay development and tesfing, screening of chemical compounds or siRNA libraries, data analysis and storage, and "hit" validafion. To gain access to the HTS facility, Cancer Center members must have a project evaluated by the Cancer Center HTS Oversight Committee, which will assess both the practicality and the scientific and therapeufic value of targets for screening. For approved projects, the HTS staff will then assess the cost and pracficality of the proposed assay and work with the inifiating laboratory to develop the assay into a form suitable for HTS that reproducibly achieves the desired signal to noise stafisties. The HTS laboratory works to minimize the cost of the experiment, which often requires changing reagents and somefimes completely redesigning the assay. Once an assay has an acceptable signal to noise ratio in small scale experiments, for compound screens mulfiple assay plates are treated with DMSO (the compound diluent) to determine reproducibility under condifions similar to HTS screening and then are screened against a test compound library to determine the "hit" rate. Genomic siRNA experiments are developed in a similar manner, except that they are run in 96 well format in replicate plates and no test library is used before the genomic screen is begun. Just as with compounds, siRNA experimental controls must achieve a reproducible signal to background rafio in runs of multiple plates before the project is allowed to screen the genomic library.