Nanoparticle Development, Production and Characterization (NP Core)

纳米粒子的开发、生产和表征（NP 核心）

• 批准号: 8310102
• 负责人: Ian Baker
• 金额: $ 10.5万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8310102
• 关键词: Behavior; Biocompatible; Biometry; CCNE1 gene; Cancer Center; Characteristics; Data Analyses; Development; Dextrans; Heating; Instruction; Iron; Letters; Magnetism; Malignant neoplasm of ovary; Measurement; Measures; Nanotechnology; Particle Size; Phase; Phospholipids; Polyethylene Glycols; Production; Property; Quality Control; Sampling; Services; System; Techniques; Transmission Electron Microscopy; Work; cancer therapy; cancer type; dextran; iron oxide; magnetic field; malignant breast neoplasm; nanocomposite; nanoparticle; novel; particle; scale up; tumor

PROJECT SUMMARY (See instructions): The Nanoparticle Development, Production and Characterization Core (NP Core) will provide well characterized nanoparticles for all four projects associated with the Dartmouth CCNE. During the start-up phase of the CCNE, we will acquire commercial dextran-coated superparamagnefic iron oxide nanoparticles (SPIOs) from Aspens Systems, Inc., Mariborough, MA (see supporting letter). These will be used initially in all the projects to jumpstart them. Forthe purposes of quality control, the core will measure both the magnetic properties and heating behavior in an applied alternating magnetic field for all the SPIOs that we will use. The main purpose ofthe core is to provide biocompatibly-coated magnetic iron/iron oxide core/shell nanocomposite particles (mNPs) that have been developed at Dartmouth. These mNPs, which have superior heating characteristics to SPIOs, will be produced with a variety of sizes (8-100 nm) as requested by each of the projects. The mNPs will be made through our well-established microemulsion techniques and a biocompatible coating of either a phospholipid or dextran and polyethylene glycol (as required by different projects) will then be assembled onto the mNPs. Transmission electron microscopy will be used to characterize the nanoparticle size and size distribution; a vibrating sample magnetometer will be used to measure the magnetic properties of the mNPs in an alternating magnefic field (for quality control); and the heating effect of the mNPs will also be measured (again for quality control). Statistical analysis of the measurements of nanoparticle size measurements, magnetic measurements and heating measurements will be performed by the Biostatistics, Data Analysis and Computational Core. Advances that will be required in the NP Core as the work progresses include the development a wider range of particle sizes (currently only 8-20 nm have been produced); and scale up of the producfion of the mNPs to produce larger quantities will be needed.

项目总结(见说明)： 纳米颗粒开发、生产和表征核心(NP Core)将为与达特茅斯CCNE相关的所有四个项目提供表征良好的纳米颗粒。在CCNE的启动阶段，我们将从马萨诸塞州马里伯勒的Aspens Systems，Inc.获得商业葡聚糖包覆的超顺磁性氧化铁纳米颗粒(SPIO)(见支持函)。这些项目最初将在所有项目中使用，以启动它们。出于质量控制的目的，磁芯将测量我们将使用的所有SPIO在外加交变磁场中的磁性和加热行为。 核心的主要目的是提供生物相容涂层的磁性铁/氧化铁核心/壳 达特茅斯大学开发的纳米复合粒子(MNPs)。这些MNPs具有比SPIO更好的加热特性，将根据每个项目的要求生产各种尺寸(8-100 nm)。 MNPs将通过我们成熟的微乳液技术制成，然后将磷脂或葡聚糖和聚乙二醇生物相容性涂层(根据不同项目的需要)组装到MNPs上。将使用透射电子显微镜来表征纳米颗粒的大小和粒度分布；将使用振动样品磁强计来测量MNPs在交变磁场中的磁性(用于质量控制)；还将测量MNPs的加热效应(再次用于质量控制)。生物统计、数据分析和计算核心将对纳米颗粒尺寸测量、磁性测量和加热测量的测量进行统计分析。 随着工作的进展，核子核需要取得的进展包括开发更大范围的颗粒尺寸(目前只生产了8-20纳米)；以及扩大纳米粒子的生产规模，以生产更大数量的纳米粒子。