MAINTENANCE AND REPAIR OF THE IVEM F30

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在本报告所述期间（2008年6月1日至2009年5月31日），F30的维修工作量最小（68小时）。我们收购 总共875个倾斜系列（165个低温和633个室温）。显微镜被大量使用 在典型的工作日（8-6 M-F），我们89%的工作在这些时间内完成。显微镜 在去年的260个可用工作日中，使用了221个。此外，显微镜使用了约30 小时/周（1553小时/52周）。F30用于室温断层扫描的占56%， 冷冻断层扫描，12%冷冻成像，4%室温成像，4%服务，2% 发展，0%用于培训。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. For this reporting period (6/1/08-5/31/09), the F30 had minimal service work (68 hours). We acquired a total of 875 tilt-series (165 cryo and 633 room temperature). The microscope was heavily used during the typical workday (8-6 M-F) with 89% of our work done during those hours. The microscope was used 221 of the 260 available workdays last year. In addition, the microscope was used about 30 hours/week (1553 hours/52 weeks). The F30 was used 56% for room temperature tomography, 22% for cryo-tomography, 12% cryo-imaging, 4% for room temperature imaging, 4% for service, 2% for development, and 0% for training.