MAINTENANCE AND REPAIR OF THE IVEM F30

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在本报告所述期间(6/1/08-5/31/09)，F30的维修工作最少(68小时)。我们获得了 共有875个倾斜系列(165个低温和633个常温)。这台显微镜用得很多 在典型的工作日(8-6M-F)，我们89%的工作都是在这些时间完成的。显微镜 在去年260个可用工作日中，有221个工作日被使用。此外，这台显微镜大约用了30分钟 每周工作时间(1553小时/52周)。F30用于室温断层扫描的比例为56%，22% 冷冻断层扫描，12%冷冻成像，4%室温成像，4%用于服务，2%用于 发展，培训为0%。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this reporting period (6/1/08-5/31/09), the F30 had minimal service work (68 hours). We acquired a total of 875 tilt-series (165 cryo and 633 room temperature). The microscope was heavily used during the typical workday (8-6 M-F) with 89% of our work done during those hours. The microscope was used 221 of the 260 available workdays last year. In addition, the microscope was used about 30 hours/week (1553 hours/52 weeks). The F30 was used 56% for room temperature tomography, 22% for cryo-tomography, 12% cryo-imaging, 4% for room temperature imaging, 4% for service, 2% for development, and 0% for training.