MASS SPECTROMETRIC STUDIES OF INTEGRAL MEMBRANE PROTEINS & ION CHANNELS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们继续开发质谱学的用途，以帮助定义完整的膜蛋白和(特别是离子通道)在原子分辨率下使用衍射方法。D.A.Doyle、J.M.Cabral、R.A.Pfuetzner、A.Kuo、J.M.Gulbis、S.L.Cohen、B.T.Chait、R.MacKinnon在《科学》280，(1998)69-77《钾通道的结构：K+传导和选择性的分子基础》一书中发表了许多出版物。麦金农，S.L.科恩，A.Kuo，A.Lee，B.T.Chait，《原核生物和真核生物钾通道的结构保护》科学280(1998)106-109。J.H.Morais Cabral，A.Lee，S.Cohen，B.T.Chait，M.Li，R.MacKinnon：《Herg钾通道氨基末端结构域的结构：PAS结构域家族的定义》Cell 95(1998)649-655。J·M·古尔比斯、M·周、S·曼、R·麦金农：《细胞质的结构(电压依赖性K+通道的亚单位-T1组装)》，科学289(2000)123-127。M.Cadene，B.T.Chait，《用于整体膜蛋白的质谱分析的强健的洗涤剂友好型》，肛门。化学。72(2000)5655-5658。S.L.Cohen，B.T.Chait，《作为研究蛋白质结构的工具的质谱学》。生物物理学与生物分子结构综述30(2001)67-85。R.duzler，ER Campbell，M.Cadene，B.T.Chait，R.MacKinnon《ClC氯通道在3.0A下的X射线结构》揭示了阴离子选择性的分子基础：自然415(2002)287-94。江亚勇，李亚平，陈军，M.Cadene，B.T.Chait，R.MacKinnon：“钙门控钾通道的晶体结构和机制”，自然杂志417(2002)515-522。姜勇，李亚平，陈军，M.Cadene，B.T.Chait，R.麦金农：《钾通道的开孔构象》，自然417(2002)523-526。姜勇，A.Lee，J.Chen，V.Ruta，M.Cadene，B.T.Chait&Roderick MacKinnon<电压相关K+通道的X射线结构>，自然423(2003)33-41；280。M.Nishida1，M.Cadene，B.T.Chait，R.MacKinnon：《Kir3.1-原核KIR通道嵌合体的晶体结构》，EMBO期刊，26(2007)4005-4015

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We continue to develop the utility of mass spectrometry to assist in the definition of integral membrane proteins and (especially ion channels) at atomic resolutions using diffraction methods. A number of publications have resulted from this work D.A. Doyle, J.M. Cabral, R.A. Pfuetzner, A. Kuo, J.M. Gulbis, S.L. Cohen, B.T. Chait, R. MacKinnon, "The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity" Science 280, (1998) 69-77. R. MacKinnon, S.L. Cohen, A. Kuo, A. Lee, B.T. Chait "Structural Conservation in Prokaryotic and Eukaryotic Potassium Channels" Science 280 (1998) 106-109. J.H. Morais Cabral, A. Lee, S. Cohen, B.T. Chait, M. Li, R. MacKinnon "Structure of the HERG potassium channel amino-terminal domain: definition of a structural family of PAS domains" Cell 95 (1998) 649-655. J.M. Gulbis, M. Zhou, S. Mann, R. MacKinnon "Structure of the Cytoplasmic ( Subunit-T1 Assembly of Voltage-Dependent K+ Channels" Science 289(2000) 123-127. M. Cadene, B.T. Chait "A Robust Detergent-Friendly for Mass Spectrometric Analysis of Integral Membrane Proteins" Anal. Chem. 72 (2000) 5655-5658. S.L. Cohen, B.T. Chait "Mass Spectrometry as a tool for Studying Protein Structure" Ann. Reviews of Biophysics and Biomolecular Structure 30 (2001) 67-85. R. Dutzler, ER Campbell, M. Cadene, B.T. Chait, R.MacKinnon "X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity" Nature 415 (2002) 287-94. Y. Jiang, A. Lee, J. Chen, M. Cadene, B. T. Chait, R. MacKinnon "Crystal structure and mechanism of a calcium-gated potassium channel" Nature 417 (2002) 515-522. Y. Jiang, A. Lee, J. Chen, M. Cadene, B. T. Chait, R. MacKinnon "The open pore conformation of potassium channels" Nature 417 (2002) 523-526. Y. Jiang, A. Lee, J. Chen, V. Ruta, M. Cadene, B.T. Chait & Roderick MacKinnon "X-ray structure of a voltage-dependent K+ channel" Nature 423 (2003) 33-41; 280. M. Nishida1, M. Cadene, B.T. Chait, R. MacKinnon "Crystal structure of a Kir3.1-prokaryotic Kir channel chimera" EMBO Journal, 26 (2007) 4005-4015