MASS SPECTROMETRIC STUDIES OF INTEGRAL MEMBRANE PROTEINS & ION CHANNELS

完整膜蛋白的质谱研究

• 批准号: 8361483
• 负责人: RODERICK MACKINNON
• 金额: $ 0.39万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361483
• 关键词: Anions; Anus; Biophysics; Calcium; Cells; Chimera organism; Chloride Channels; Cytoplasmic Structures; Detergents; Family; Funding; Grant; Integral Membrane Protein; Ion Channel; Journals; Mass Spectrum Analysis; Methods; Molecular; Molecular Conformation; National Center for Research Resources; Nature; Potassium Channel; Principal Investigator; Publications; Research; Research Infrastructure; Resolution; Resources; Roentgen Rays; Ruta; Science; Source; Structure; United States National Institutes of Health; Work; base; cost; macromolecule; protein structure; tool; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We continue to develop the utility of mass spectrometry to assist in the definition of integral membrane proteins and (especially ion channels) at atomic resolutions using diffraction methods. A number of publications have resulted from this work D.A. Doyle, J.M. Cabral, R.A. Pfuetzner, A. Kuo, J.M. Gulbis, S.L. Cohen, B.T. Chait, R. MacKinnon, "The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity" Science 280, (1998) 69-77. R. MacKinnon, S.L. Cohen, A. Kuo, A. Lee, B.T. Chait "Structural Conservation in Prokaryotic and Eukaryotic Potassium Channels" Science 280 (1998) 106-109. J.H. Morais Cabral, A. Lee, S. Cohen, B.T. Chait, M. Li, R. MacKinnon "Structure of the HERG potassium channel amino-terminal domain: definition of a structural family of PAS domains" Cell 95 (1998) 649-655. J.M. Gulbis, M. Zhou, S. Mann, R. MacKinnon "Structure of the Cytoplasmic ( Subunit-T1 Assembly of Voltage-Dependent K+ Channels" Science 289(2000) 123-127. M. Cadene, B.T. Chait "A Robust Detergent-Friendly for Mass Spectrometric Analysis of Integral Membrane Proteins" Anal. Chem. 72 (2000) 5655-5658. S.L. Cohen, B.T. Chait "Mass Spectrometry as a tool for Studying Protein Structure" Ann. Reviews of Biophysics and Biomolecular Structure 30 (2001) 67-85. R. Dutzler, ER Campbell, M. Cadene, B.T. Chait, R.MacKinnon "X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity" Nature 415 (2002) 287-94. Y. Jiang, A. Lee, J. Chen, M. Cadene, B. T. Chait, R. MacKinnon "Crystal structure and mechanism of a calcium-gated potassium channel" Nature 417 (2002) 515-522. Y. Jiang, A. Lee, J. Chen, M. Cadene, B. T. Chait, R. MacKinnon "The open pore conformation of potassium channels" Nature 417 (2002) 523-526. Y. Jiang, A. Lee, J. Chen, V. Ruta, M. Cadene, B.T. Chait & Roderick MacKinnon "X-ray structure of a voltage-dependent K+ channel" Nature 423 (2003) 33-41; 280. M. Nishida1, M. Cadene, B.T. Chait, R. MacKinnon "Crystal structure of a Kir3.1-prokaryotic Kir channel chimera" EMBO Journal, 26 (2007) 4005-4015

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们继续开发质谱的实用性，以帮助定义完整的膜蛋白和（特别是离子通道）在原子分辨率使用衍射方法。D.A. Doyle，J.M. Cabral，R.A. Pfuetzner，A.郭，J.M.古尔比斯公司科恩，B.T.柴特河MacKinnon，“钾通道的结构：K+传导和选择性的分子基础”Science 280，（1998）69-77。 R. MacKinnon，S.L.科恩，A. Kuo，黄毛菊A.李，B.T. Chait，“Proteins and Eukaryotic Potassium Channels”，Science 280（1998）106-109。 J.H. Morais Cabral，A.李，S。 科恩，B.T.查特，M.利河，巴西-地MacKinnon“Structure of the HERG potassium channel amino-terminal domain：definition of a structural family of PAS domains”Cell 95（1998）649-655. J.M. Gulbis，M. Zhou，S.曼河MacKinnon“电压依赖性K+通道的细胞质（亚单位-T1组装）的结构”Science 289（2000）123-127。 M. Cadene，BT Chait“A Robust Detergent-Friendly for Mass Spectrometric Analysis of Integral Membrane Proteins”Anal. 72（2000）5655-5658。 S.L.科恩，B.T.质谱分析法作为研究蛋白质结构的工具>，生物物理学与生物分子结构回顾，30（2001）67-85。R. Dutzler，ER坎贝尔，M. Cadene，BT Chait，R.MacKinnon“X-ray structure of a C1C chloride channel at 3.0 A reveals the molecular basis of anion selectivity”Nature 415（2002）287-94. Y. Jiang，中国茶条A. Lee，J. Chen，M. Cadene，B. T.柴特河MacKinnon“Crystal structure and mechanism of a calcium-gated potassium channel”Nature 417（2002）515-522. Y. Jiang，中国茶条A. Lee，J. Chen，M. Cadene，B. T.柴特河MacKinnon“钾通道的开放孔构象”Nature 417（2002）523-526。Y. Jiang，中国茶条A. Lee，J. Chen，V. Ruta，M. Cadene，B.T. Chait & Roderick MacKinnon“电压依赖性K+通道的X射线结构”Nature 423（2003）33-41; 280. M. Nishida 1，M. Cadene，B.T.柴特河MacKinnon“Crystal structure of a Kir3.1-prokaryotic Kir channel chimera”EMBO Journal，26（2007）4005-4015