Very Low Frequency EPR Imaging in Vivo Phisiology P41 EB02034

描述（由申请人提供）：分子氧合图像的高分辨率图像可以为癌症治疗的输送和监测提供重要指导。氧的EPR成像（EPRI）提供了空间分辨率、氧分辨率和组织中深度的均匀灵敏度的独特组合。该中心的重点是优化体内EPRI氧气成像预期人体图像。这是一个由芝加哥、马里兰州和丹佛大学组成的联盟。成功的图像需要协调开发仪器以获得图像，旋转探针以采样和报告组织液环境，以及成像策略以最佳地采样和分析所获得的信息。该中心建立在工程师，生物学家，放射肿瘤学家，医学物理学家，物理和有机化学家，统计学家和成像数学家的多学科努力之上。在过去的21/2年中，该中心1）逐点验证了EPRI氧气图像，2）开发了氧气与解剖学和其他生理学的共配准，3）增加了成像的动物肿瘤大小，4）产生了第一个电子自旋回波氧气图像，其速度比连续波（CW）采集快近一个数量级，5）开发了能够批量合成的窄线三苯甲基自旋探针源，6）合成持续的细胞内氮氧自由基和7）从根本上改变了从投影的图像重建，允许在均匀维度上的部分投影。这些成就直接改善了空间、氧合和/或时间图像分辨率。生理相关的分辨率目标要求提高小动物EPRI氧气图像的分辨率。这种改进对于扩大到更大体积的组织和肿瘤的成像是必要的。我们建议： 1)实施多种CW采集技术，以提高投影采集率，选择感兴趣区域并构建新型谐振器 2)对于脉冲图像采集，增加带宽和带宽均匀性以及新颖的谐振器和技术来选择感兴趣的区域 3)开发新型、更窄的线自旋探针，具有更高的氧灵敏度 4)建立在图像采集和分析进展的基础上。 我们希望在支持的第五年获得一个人类形象。

DESCRIPTION (provided by applicant): High resolution images of molecular oxygenation images can provide crucial guides to the delivery and monitoring of cancer therapy. EPR imaging (EPRI) of oxygen provides a unique combination of spatial resolution, oxygen resolution, and uniform sensitivity with depth in tissue. This Center focuses on the optimization of In Vivo EPRI oxygen imaging anticipating human images. It is a consortium between the Universities of Chicago, Maryland and Denver. Successful images require coordinated development of instrumentation to obtain the images, spin probes to sample and report the tissue fluid environment and imaging strategies to optimally sample and analyze the information obtained. The Center is built on multidisciplinary effort from engineers, biologists, radiation oncologists, medical physicists, physical and organic chemists, statisticians and imaging mathematicians. In the past 21/2 years the Center 1) validated EPRI oxygen images point by point, 2) developed co-registration of oxygen with anatomy and other physiology, 3) increased animal tumor size imaged, 4) produced the first electron spin echo oxygen image nearly an order of magnitude faster than continuous wave (CW) acquisition, 5) developed a source of narrow line trityl spin probe capable of bulk synthesis, 6) synthesized persistent intracellular nitroxides and 7) radically altered reconstruction of images from projections allowing partial projections in even dimensions. These achievements directly improve spatial, oxygenation and/or temporal image resolution. Physiologically relevant resolution goals mandates improvement in resolution of EPRI oxygen images for small animals. This improvement will be necessary to scale to imaging larger volumes of tissue and tumor. We propose to: 1) Implement multiple techniques for CW acquisition to increase projection acquisition rate, select regions of interest and construct novel resonators 2) For pulsed image acquisition increase bandwidth and bandwidth uniformity as well as novel resonators and techniques to select regions of interest 3) Develop novel, even narrower line spin probes to with higher oxygen sensitivity 4) Build on the image acquisition and analysis progress. We hope to acquire a human image in the 5th year of support.