Molecular mechanisms underlying G protein coupled receptor signaling

G蛋白偶联受体信号转导的分子机制

• 批准号: nhmrc : 299811
• 负责人: Karin Eidne
• 金额: $ 39.81万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/molecular-mechanisms-underlying-receptor-signaling/76714
• 关键词: Molecular; mechanisms; underlying; protein; coupled

The maintenance of optimum health and function of living cells, and consequently that of the whole organism, depends on how cells respond to a multitude of physical and chemical stimuli that continually bombard them. The majority of the chemical stimuli such as hormones and neurotransmitters impart their actions not by directly entering the cell, but instead, by binding to a specific receiver protein at the cell surface called a receptor. In one class of such receptors called G protein-coupled receptors, the transmission of the message to the interior of the cell involves yet another protein called G protein. These receptors are the most abundant type of cell surface receptors and form the targets for nearly 50% of currently used therapeutic drugs. It is, therefore, extremely important to unravel how each of these components works, and in particular to know how they work in living cells. This project utilizes state-of-the-art methodologies to examine interactions between receptors and their cognate G proteins, in living cells and in real-time. The work will answer fundamental questions about the nature of G protein-coupled receptor signaling and will aid in the future development of more effective therapeutic agents.

活细胞乃至整个有机体的最佳健康和功能的维持，取决于细胞如何对不断轰击它们的大量物理和化学刺激做出反应。大多数化学刺激物（例如激素和神经递质）不是通过直接进入细胞来发挥作用，而是通过与细胞表面称为受体的特定接收蛋白结合来发挥作用。在一类称为 G 蛋白偶联受体的受体中，向细胞内部传递信息涉及另一种称为 G 蛋白的蛋白质。这些受体是最丰富的细胞表面受体类型，构成了近 50% 目前使用的治疗药物的靶标。因此，弄清楚这些成分的工作原理，特别是了解它们在活细胞中的工作原理，是极其重要的。该项目利用最先进的方法来实时检查活细胞中受体与其同源 G 蛋白之间的相互作用。这项工作将回答有关 G 蛋白偶联受体信号传导性质的基本问题，并将有助于未来开发更有效的治疗药物。