Photonic structures for direct ultrasensitive virus detection

用于直接超灵敏病毒检测的光子结构

• 批准号: 7802206
• 负责人: Benjamin L Miller
• 金额: $ 34.14万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-15 至 2013-03-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7802206
• 关键词: Address; Antibodies; Area; Bacteria; Bacterial Spores; Biological; Biological Assay; Biological Markers; Biological Models; Biosensor; Capsid Proteins; Complex; Coupled; Defect; Detection; Development; Devices; Epitopes; Excision; Filtration; HIV; Health; Human; Immobilization; Indium; Infection; Influenza; Influenza A Virus, H5N1 Subtype; Label; Laboratories; Latex Bead; Liquid substance; Location; Mediating; Membrane; Methodology; Methods; Microfluidics; Monitor; Oligonucleotides; Optics; Particle Size; Pathogen detection; Pattern; Phase; Polymerase; Polymers; Production; Protein Chemistry; Proteins; Reagent; Research; Research Personnel; SARS coronavirus; Sampling; Savings; Serum; Severe Acute Respiratory Syndrome; Silicon; Smallpox; Solutions; Source; Specificity; Structure; Surface; System; Techniques; Technology; Temperature; Testing; Time; Vaccinia; Vaccinia virus; Viral; Virion; Virulence; Virus; Virus Diseases; Work; antiretroviral therapy; base; cost; design; his6 tag; instrumentation; interest; nanolithography; nanoparticle; nanoscale; new technology; novel; particle; pathogen; photonics; public health relevance; sensor; single molecule; submicron; success; two-dimensional; viral detection

DESCRIPTION (provided by applicant): Direct single- or near-single copy detection of viruses is an important challenge in human health. Applications include the need to monitor emerging viral threats (H5N1 influenza and the SARS virus, for example), and the importance of understanding the long-term implications of nearly undetectable reservoirs of infection following antiretroviral therapy for HIV. While considerable effort has been expended on the development of ultrasensitive detection methods, the need remains for new technologies that are exceptionally sensitive, label-free, physically robust, and inexpensive. We hypothesize that two-dimensional photonic bandgap (2DPBG) sensors can fulfill all of these requirements. We will test that hypothesis by completing the following three Aims. In Aim 1, we will develop and test a new method for nanoscale patterning of single chain antibodies, and will implement that method in single-target 2DPBG sensors for vaccinia. In Aim 2, we will extend this methodology to the production of dual-target sensors. Finally, we will integrate these novel biosensors with microfluidic channels and with features for sample filtration and/or preconcentration in Aim 3. PUBLIC HEALTH RELEVANCE: Current methods for detecting viruses with high sensitivity - down to the single viral particle level - are expensive and complex. We propose to develop and test a new class of optical biosensors based on silicon that will be able to detect viruses simply and rapidly, with single-particle sensitivity. These new sensors will have applications in the detection of HIV, and emerging viral pathogens such as H5N1 influenza and SARS, among others.

描述（由申请方提供）：病毒的直接单拷贝或近单拷贝检测是人类健康面临的重要挑战。应用包括需要监测新出现的病毒威胁（例如H5 N1流感和SARS病毒），以及了解艾滋病毒抗逆转录病毒治疗后几乎无法检测的感染宿主的长期影响的重要性。虽然在开发超灵敏检测方法方面已经付出了相当大的努力，但仍然需要特别灵敏、无标记、物理上坚固和廉价的新技术。我们假设二维光子带隙（2DPBG）传感器可以满足所有这些要求。我们将通过完成以下三个目标来验证这一假设。 在目标1中，我们将开发和测试一种用于单链抗体纳米级图案化的新方法，并将在用于牛痘的单靶2DPBG传感器中实施该方法。在目标2中，我们将把这种方法扩展到双目标传感器的生产。最后，我们将把这些新的生物传感器与微流体通道和目标3中的样品过滤和/或预浓缩功能相结合。公共卫生相关性：目前用于以高灵敏度检测病毒（低至单个病毒颗粒水平）的方法昂贵且复杂。我们建议开发和测试一类新的基于硅的光学生物传感器，它将能够简单快速地检测病毒，具有单粒子灵敏度。这些新的传感器将应用于检测艾滋病毒和新出现的病毒病原体，如H5 N1流感和SARS等。