MHC TYPING OF MACAQUES USED IN AIDS RESEARCH

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的：为从事恒河猴(印度恒河猴、中国恒河猴和食蟹猴)研究人员提供MHC I、II类基因座的MHC分型。将人类白细胞抗原分型技术应用于恒河猴(印度恒河猴、中国恒河猴和食蟹猴)MHC I和II类的分子分型。 在HIV疫苗开发和致病机理研究中，各种猕猴作为动物模型的实用性增加，因此有必要继续为这些物种建立参考MHC分型实验室。我们计划继续为北美和欧洲科学界提供服务，对猕猴进行MHC分型。最初，这将包括对编码MHC I类和II类分子的等位基因进行PCR-SSP测试，这些分子结合来自SIV和SIV的多肽。我们正在开发更多的分子技术来分析印度恒河猴、中国恒河猴和食蟹猴的MHC I类和II类等位基因。此外，我们还为希望建立MHC打字系统的各个实验室提供培训。最后，我们正在开发一组特性良好的细胞系，这对分析猕猴体内的MHC将是无价的。从2002年到现在，我们已经为50个机构的130多名研究人员进行了超过12万次打字。2008年，应来自15个不同机构的32名首席调查员的要求，我们进行了23 500次打字。此外，我们已经开发并准备在2009年提供扩展的分型服务，其中包括印度恒河猴总共35个I类和20个II类等位基因的特异性分型。这项研究使用了WNPRC免疫遗传学和病毒学服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To offer MHC typing of the MHC class I and II loci for investigators working with macaques (Indian rhesus, Chinese rhesus, and Cynomolgus). To adapt technologies for HLA typing to molecular typing of macaque (Indian rhesus, Chinese rhesus, and Cynomolgus) MHC class I and II. The increased utility of various species of macaques as animal models in both HIV vaccine development and pathogenesis studies necessitates the continuation of reference MHC typing laboratories for these species. We plan to continue to offer services to both the North American and European scientific communities for MHC typing of macaques. Initially, this will include PCR-SSP tests for alleles encoding MHC class I and II molecules that bind peptides derived from SIV and SHIV. We are developing additional molecular techniques for analysis of the Indian rhesus and Chinese rhesus and Cynomolgus macaque MHC class I and class II alleles. Additionally, we offer training for individual laboratories that wish to set up MHC typing. Finally, we are developing a panel of well-characterized cell lines that will be invaluable for the analysis of the MHC in the macaque. Since 2002 to the present, we have performed over 120,000 typings for over 130 investigators at 50 institutions. In 2008, we have conducted 23,500 typings at the request of 32 principal investigators from 15 different institutions. In addition, we have developed and are preparing to offer expanded typing services during 2009, which include typing specificities for a total of ~35 Class I and ~20 Class II allele specificities of the Indian rhesus macaque. This research used WNPRC Immunogenetics & Virology Services.