MHC TYPING OF MACAQUES USED IN AIDS RESEARCH

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 目的：为猕猴（印度恒河猴、中国恒河猴和食蟹猴）的MHC Ⅰ类和Ⅱ类基因座分型提供依据。使HLA分型技术适用于猕猴（印度恒河猴、中国恒河猴和食蟹猴）MHC I类和II类分子分型。 在HIV疫苗开发和发病机制研究中，各种猕猴作为动物模型的实用性越来越高，需要继续参考这些物种的MHC分型实验室。我们计划继续为北美和欧洲科学界提供猕猴MHC分型服务。最初，这将包括对编码MHC I类和II类分子的等位基因进行PCR-SSP测试，这些分子结合来自SIV和SHIV的肽。我们正在开发用于分析印度恒河猴、中国恒河猴和食蟹猴MHC I类和II类等位基因的其他分子技术。此外，我们还为希望建立MHC分型的个别实验室提供培训。最后，我们正在开发一组特征良好的细胞系，这将是非常宝贵的猕猴MHC的分析。自2002年至今，我们已为50家机构的130多名研究者进行了超过120，000次打字。2008年，我们应来自15个不同机构的32名主要调查员的要求进行了23 500次打字。此外，我们已经开发并准备在2009年期间提供扩展的分型服务，其中包括印度恒河猴的~35个I类和~20个II类等位基因特异性的分型特异性。这项研究使用WNPRC免疫遗传学和病毒学服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To offer MHC typing of the MHC class I and II loci for investigators working with macaques (Indian rhesus, Chinese rhesus, and Cynomolgus). To adapt technologies for HLA typing to molecular typing of macaque (Indian rhesus, Chinese rhesus, and Cynomolgus) MHC class I and II. The increased utility of various species of macaques as animal models in both HIV vaccine development and pathogenesis studies necessitates the continuation of reference MHC typing laboratories for these species. We plan to continue to offer services to both the North American and European scientific communities for MHC typing of macaques. Initially, this will include PCR-SSP tests for alleles encoding MHC class I and II molecules that bind peptides derived from SIV and SHIV. We are developing additional molecular techniques for analysis of the Indian rhesus and Chinese rhesus and Cynomolgus macaque MHC class I and class II alleles. Additionally, we offer training for individual laboratories that wish to set up MHC typing. Finally, we are developing a panel of well-characterized cell lines that will be invaluable for the analysis of the MHC in the macaque. Since 2002 to the present, we have performed over 120,000 typings for over 130 investigators at 50 institutions. In 2008, we have conducted 23,500 typings at the request of 32 principal investigators from 15 different institutions. In addition, we have developed and are preparing to offer expanded typing services during 2009, which include typing specificities for a total of ~35 Class I and ~20 Class II allele specificities of the Indian rhesus macaque. This research used WNPRC Immunogenetics & Virology Services.