COMPARATIVE GENOMICS OF PARASITIC NEMATODES

寄生线虫的比较基因组学

• 批准号: 8076271
• 负责人: Makedonka Mitreva
• 金额: $ 30.82万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-23 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8076271
• 关键词: Active Sites; Amino Acid Sequence; Anthelmintics; Automobile Driving; Basic Science; Binding; Biochemical Pathway; Bioinformatics; Biological; Buffers; C. elegans genome; Cellular biology; Classification; Communities; Complementary DNA; Complex; Computer Simulation; Data; Data Analyses; Data Set; Databases; Development; Diagnostic; Drug Delivery Systems; Endocrine system; Enzymes; Evolution; Expressed Sequence Tags; Genes; Genome; Genomics; Goals; Homologous Gene; Hookworms; Human; Informatics; Information Dissemination; Intervention; Ions; Lead; Metabolic; Metabolic Pathway; Metabolism; Molecular; Morbidity - disease rate; National Human Genome Research Institute; Nature; Nematoda; Outcome; Parasite Control; Parasites; Parasitic nematode; Parasitology; Pathway interactions; Peptide Sequence Determination; Pharmaceutical Preparations; Phosphorylation Site; Population; Post-Translational Protein Processing; Process; Protein Family; Proteins; Reaction; Reagent; Research; Resistance; Resources; Running; Screening procedure; Secure; Signal Pathway; Staging; Subcategory; Surveys; Taxon; Testing; Time; Tissues; Toxic effect; Universities; Vaccine Antigen; Vaccines; Washington; base; comparative genomics; empowered; filaria; genome sequencing; genome-wide; human morbidity; improved; information processing; insertion/deletion mutation; model organisms databases; mortality; novel; novel diagnostics; novel therapeutics; parasite genome; pathogen; programs; public health relevance; research and development; tool

DESCRIPTION (provided by applicant): Parasitic nematodes infect over half the world's population, resulting in significant morbidity and mortality. Characterization of nematode genomes provides fundamental molecular information about these parasites accelerating basic research and development of new diagnostics and therapeutics. Washington University's Genome Center has generated and made public over 400,000 cDNAs from 30 parasitic species, sequenced 4 genomes to draft coverage with ten more underway including representatives of the major human parasitic groups. The three aims in this proposal analyze the expanding nematode sequences to substantially improve understanding of parasitic nematode biology and cellular pathways. First, we will develop and use bioinformatic tools to process, assemble, and annotate incoming data from all sequencing platforms. These genomic resources will also be disseminated to the wider research community through the centralized parasitic nematode database, Nematode.net. Second, analysis will focus on biochemical pathways conserved and/or taxonomically restricted including proteins that may prove useful as drug targets. Third, we will study the nature and implications of nematode-specific insertions and deletions in proteins involved in environmental information processing and endocrine system. The expected outcome will facilitate and promote the discovery and development of novel interventions to control these important parasites and reduced their associated morbidity and mortality. PUBLIC HEALTH RELEVANCE: The continued development of molecular information, bioinformatics tools, and reagents for the study of parasitic nematodes is crucial, as they infect over half of the world's population and are a leading cause of human morbidity. The main goal of this project is to implement comparative genomics approaches to study the biology and cellular pathways of these important parasites, which on a long run will contribute to improved diagnostics, vaccines, and anthelmintic drugs for broad parasite control.

描述（由申请人提供）：寄生线虫感染了世界上一半以上的人口，导致显著的发病率和死亡率。线虫基因组的表征提供了关于这些寄生虫的基本分子信息，加速了基础研究和新诊断和治疗方法的开发。华盛顿大学的基因组中心已经生成并公开了来自30种寄生虫物种的40多万个cDNA，对4个基因组进行了测序，以起草覆盖范围，还有10个正在进行中，包括主要人类寄生虫群体的代表。该提案的三个目标是分析不断扩展的线虫序列，以大大提高对寄生线虫生物学和细胞途径的理解。首先，我们将开发和使用生物信息学工具来处理，组装和注释来自所有测序平台的传入数据。这些基因组资源还将通过集中的寄生线虫数据库Nematode.net传播给更广泛的研究界。其次，分析将集中在保守和/或分类学限制的生化途径，包括可能被证明可用作药物靶点的蛋白质。第三，我们将研究线虫特异性插入和缺失的蛋白质参与环境信息处理和内分泌系统的性质和意义。预期的结果将促进和促进新的干预措施的发现和发展，以控制这些重要的寄生虫，并降低其相关的发病率和死亡率。 公共卫生相关性：继续开发用于寄生线虫研究的分子信息、生物信息学工具和试剂至关重要，因为它们感染了世界上一半以上的人口，是人类发病的主要原因。该项目的主要目标是实施比较基因组学方法来研究这些重要寄生虫的生物学和细胞途径，从长远来看，这将有助于改进诊断，疫苗和驱虫药物，以广泛控制寄生虫。