Water and solute transport in the epididymis
附睾中水和溶质的运输
基本信息
- 批准号:8090009
- 负责人:
- 金额:$ 11.47万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-07-14 至 2011-06-30
- 项目状态:已结题
- 来源:
- 关键词:AcuteAdultAntibodiesApicalBiological AssayCell VolumesCellsChimeric ProteinsCo-ImmunoprecipitationsCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorDataDuct (organ) structureDuctal EpitheliumEnvironmentEpididymisEpithelial CellsEpitheliumFertilityGelGenerationsGlycerolHarvestImmunofluorescence ImmunologicIn Situ HybridizationIn VitroIsotonic ExerciseLaboratoriesLiquid substanceMannitolMeasurementMeasuresMessenger RNAOrganOsmolar ConcentrationOsmotic ShocksPathway interactionsPermeabilityPhloretinPhosphorylationProcessProteinsRattusRegulationResearch PersonnelReverse Transcriptase Polymerase Chain ReactionRoleSamplingScreening procedureSiteSorbitolStaining methodStainsTestingTissuesVariantVesicleWaterWater MovementsWestern Blottingapical membranebasolateral membranebrush border membranecell motilitycell typecryostatdigital imagingin vivoinhibitor/antagonistinteininterdisciplinary approachlaser capture microdissectionlight scatteringmalenovelprogramsprotein expressionprotein protein interactionreproductivesolutesperm cellwater channelwater flowzygote
项目摘要
DESCRIPTION: Fluid reabsorption across epithelial cells of the excurrent ducts of the male reproductive tract results in an increase in sperm concentration that is crucial for fertility. The mechanisms underlying the regulation of this process are poorly understood. AQP9 has been identified as the major apical water channel in the epididymis. However its role in epididymal transepithelial transport has not been investigated. AQP9 is an aquaglyceroporin that can transport neutral solutes in addition to water. This application is aimed at
characterizing the function and regulation of AQP9-related water and solute transport in the epididymis. SPECIFIC AIM 1 will test two hypotheses: 1) AQP9 participates in apical water and/or neutral solute transport and is responsible for the establishment of an optimum luminal osmolarity as well as spermatozoa concentration; and 2) The basolateral membrane of the epididymal epithelium contains an as yet unidentified aquaporin(s). We will characterize the pathways of apical and basolateral water and/or solute transport, using a multidisciplinary approach including epididymal tubules perfused in vivo and in vitro, quantitative digital imaging (cell volume measurement and immunofluorescence), and stopped-flow light scattering in apical membrane vesicles. We will identify the basolateral aquaporin(s) that must participate in water and/or solute flow across the epididymal epithelium by homology screening from epithelial cells isolated by laser capture microdissection. Interestingly, since the previous submission, we have shown that AQP11 mRNA is expressed in the epididymal epithelium. We will now study AQP11 mRNA localization by in situ hybridization, and protein expression by western blotting and immunofluorescence. SPECIFIC AIM 2 will test whether AQP9 is regulated via: 1) direct phosphorylation; or 2) interaction with partner proteins. We will study the acute regulation of AQP9 via phosphorylation using purified AQP9. Protein-protein interaction involving
"PDZ" domains will be examined using pull-down, co-immunoprecipitation and gel overlay assays. Interactions with CFTR, NHERF1 or PDZK1 will be studied. The proposed studies will provide unique and novel information that is important to our understanding of how water and solute transport across the excurrent duct epithelium contribute to the generation of a luminal environment in which spermatozoa mature, are stored, and are concentrated.
男性生殖道外流管上皮细胞间的液体重吸收导致精子浓度增加,这对生育能力至关重要。这一过程的调控机制尚不清楚。AQP9被认为是附睾最主要的顶端水通道。然而,其在附睾经上皮运输中的作用尚未被研究。AQP9是一种除水外还能运输中性溶质的水甘油oporin。这个应用程序的目标是
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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SYLVIE BRETON其他文献
SYLVIE BRETON的其他文献
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{{ truncateString('SYLVIE BRETON', 18)}}的其他基金
pH-sensing and activation of acid secretion by the V-ATPase
V-ATP 酶的 pH 感应和酸分泌激活
- 批准号:
8593292 - 财政年份:2012
- 资助金额:
$ 11.47万 - 项目类别:
pH-sensing and activation of acid secretion by the V-ATPase
V-ATP 酶的 pH 感应和酸分泌激活
- 批准号:
8777956 - 财政年份:2012
- 资助金额:
$ 11.47万 - 项目类别:
pH-sensing and activation of acid secretion by the V-ATPase
V-ATP 酶的 pH 感应和酸分泌激活
- 批准号:
8419239 - 财政年份:2012
- 资助金额:
$ 11.47万 - 项目类别:
Role of epididymal macrophages and dendritic cells in male reproductive function
附睾巨噬细胞和树突状细胞在男性生殖功能中的作用
- 批准号:
9274090 - 财政年份:2011
- 资助金额:
$ 11.47万 - 项目类别:
3-Dimensional modeling of basal cell function in pseudostratified epithelia
假复层上皮基底细胞功能的三维建模
- 批准号:
8327225 - 财政年份:2009
- 资助金额:
$ 11.47万 - 项目类别:
3-Dimensional modeling of basal cell function in pseudostratified epithelia
假复层上皮基底细胞功能的三维建模
- 批准号:
7764187 - 财政年份:2009
- 资助金额:
$ 11.47万 - 项目类别:
3-Dimensional modeling of basal cell function in pseudostratified epithelia
假复层上皮基底细胞功能的三维建模
- 批准号:
8541004 - 财政年份:2009
- 资助金额:
$ 11.47万 - 项目类别:
3-Dimensional modeling of basal cell function in pseudostratified epithelia
假复层上皮基底细胞功能的三维建模
- 批准号:
7936862 - 财政年份:2009
- 资助金额:
$ 11.47万 - 项目类别:
3-Dimensional modeling of basal cell function in pseudostratified epithelia
假复层上皮基底细胞功能的三维建模
- 批准号:
8131595 - 财政年份:2009
- 资助金额:
$ 11.47万 - 项目类别:
MECHANISMS OF LUMINAL ACIDIFICATION IN EPIDYDIMAS & VAS DEFERENS
附睾管腔酸化机制
- 批准号:
7953821 - 财政年份:2008
- 资助金额:
$ 11.47万 - 项目类别:
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