MECHANISMS OF LUMINAL ACIDIFICATION IN EPIDYDIMAS & VAS DEFERENS

附睾管腔酸化机制

• 批准号: 7953821
• 负责人: SYLVIE BRETON
• 金额: $ 0.56万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953821
• 关键词: Bicarbonates; Cell membrane; Clear Cell; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Duct (organ) structure; Electrodes; Environment; Enzymes; Epididymis; Epithelial Cells; Fertility; Funding; Goals; Grant; Institution; Intervention; Laboratories; Organ; Pathologic; Prevention; Process; Proton Pump; Proton-Translocating ATPases; Protons; Recycling; Regulation; Reproductive Physiology; Research; Research Personnel; Resources; Source; Sperm Maturation; United States National Institutes of Health; Vas deferens structure; Vesicle; apical membrane; male; premature; reproductive; reproductive function; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Active proton transport mechanisms establish and maintain a luminal acidic environment in the excurrent ducts of the male reproductive tract. The low luminal pH and a low bicarbonate concentration are important for sperm maturation, the prevention of premature activation of acrosomal enzymes, and for maintaining sperm in a quiescent state during storage in this organ. Despite this critical function in male reproductive physiology, acidification processes in the male reproductive tract are still incompletely understood. Our laboratory is studying the mechanisms that participate in luminal acidification and its regulation in the epididymis and vas deferens. The ultimate goals of this study are to understand how defective acidification may impair reproductive function in pathologic states, and how intervention to manipulate acidification may eventually be used to control male fertility. The non-invasive self-referencing proton-selective micro electrode developed in the BioCurrrents Research Center has allowed us to identify the major proton secretory mechanism in the initial part of the vas deferens. In collaboration with Peter Smith, we have shown that a bafilomycin-sensitive, vacuolar proton-pumping H+-ATPase (V-ATPase) is concentrated in the apical membrane of a subpopulation of epithelial cells (clear cells) and is a major contributor to luminal acidification. In acidifying clear cells, proton secretion is regulated via recycling of V-ATPase-containing vesicles to and from the plasma membrane. .

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 活跃的质子转运机制在男性生殖道的出现管道中建立并维持腔酸性环境。低腔pH值和低碳酸氢盐浓度对于精子成熟，预防过早激活Arosomal酶以及在该器官存储期间以静态状态保持精子。尽管在男性生殖生理学中的关键功能，但仍未完全了解男性生殖道的酸化过程。我们的实验室正在研究参与腔酸化的机制及其在附睾和VAS延迟中的调节。这项研究的最终目标是了解有缺陷的酸化可能如何损害病理状态的生殖功能，以及如何最终可以使用干预酸化来控制男性生育能力。 生物含量研究中心开发的非侵入性自我引用质子选择性微电极使我们能够在VAS延迟的最初部分识别主要的质子分泌机制。我们与彼得·史密斯（Peter Smith）合作，我们表明，对bafilomycin敏感的，液泡质子泵化的H+-ATPase（V-ATPase）集中在上皮细胞亚群的顶膜上（透明细胞），是腔酸酸化的主要影响。在酸化的透明细胞中，质子分泌通过回收含V-ATPase的囊泡和从质膜进行调节。 。