Nanosensor device for the detection of H. Pylori and prevention of gastric cancer

用于检测幽门螺杆菌和预防胃癌的纳米传感器装置

• 批准号: 8114329
• 负责人: Anastasia Rigas
• 金额: $ 15.23万
• 依托单位: NANOMEDICON, LLC
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8114329
• 关键词: Air; Ammonia; Antibiotics; Bacteria; Biological Assay; Breath Tests; Carbon Dioxide; Clinical; Clinical Management; Data; Detection; Developing Countries; Development; Devices; Diagnosis; Diagnostic tests; Disease; Emerging Technologies; Enzymes; Gastric Adenocarcinoma; Gastric lymphoma; Goals; Hand; Healthcare; Helicobacter Infections; Helicobacter pylori; Human; Human Resources; Infection; Label; Lymphoma; Medical; Methods; Monitor; Nanotechnology; Numerical value; Organism; Patients; Peptic Ulcer; Population; Price; Proteins; Public Health; Risk; Sampling; Sensitivity and Specificity; Testing; Treatment Efficacy; Urea; Urease; Work; base; cost; gastric cancer prevention; human disease; instrument; instrumentation; malignant stomach neoplasm; molybdenum trioxide; mucosa-associated lymphoid tissue lymphoma; nanosensors; novel; point of care; prevent; sensor

DESCRIPTION (provided by applicant): We propose to develop a novel method for the diagnosis of Helicobacter Pylori. The diagnosis of H. Pylori is a high medical priority because: this bacterium infects approximately 50% of the world's population; causes peptic ulcer disease, gastric cancer, a form of lymphoma (MALToma) and likely several other diseases; and is treatable with antibiotics, but if this organism is not eradicated, patients remain at risk for serious complications. Of the many diagnostic tests for H Pylori, the urea breath test (UBT) is the best: it is simple, noninvasive, has excellent sensitivity and specificity and can diagnose current infection. It is based on the ability of H pylori to convert urea to CO2 and ammonia using its enzyme urease. Current embodiments of UBT employ 13C-urea and detect labeled CO2 in breath. Current UBTs are not extensively used because they are costly and not available at the point-of-care. We propose to develop a simple UBT using as substrate unlabeled urea and detecting ammonia in breath with a nanosensor incorporated in a very simple device. Our preliminary work fully supports the feasibility of our approach. Our specific aims are to 1) Perform UBTs in patients using our sensor to determine NH3, and 2) Develop a portable breath analyzer for the detection of H. pylori based on the determination of NH3 in human breath. The proposed UBT will be simple, inexpensive, noninvasive and available at the point-of care. Thus it will make widely available a simple device for the diagnosis of H pylori and the monitoring of treatment efficacy. PUBLIC HEALTH RELEVANCE: We propose to develop a simple-to-use, inexpensive and point of care device to rapidly diagnose infection with Helicobacter pylori. This organism infects approximately 50% of the world's population and causes peptic ulcer. It has also been associated with the development of gastric cancer. The device's impact on public health will be immediate and major.

描述（由申请人提供）：我们建议开发一种诊断幽门螺杆菌的新方法。H.幽门螺杆菌是一个高度医疗优先事项，因为：这种细菌感染了大约50%的世界人口;导致消化性溃疡疾病，胃癌，一种淋巴瘤（MALToma）和可能的其他几种疾病;并且可以用抗生素治疗，但如果这种生物体不被根除，患者仍然有严重并发症的风险。在幽门螺杆菌的许多诊断试验中，尿素呼气试验（UBT）是最好的：它简单，无创，具有良好的敏感性和特异性，可以诊断当前感染。它是基于幽门螺杆菌使用其尿素酶将尿素转化为CO2和氨的能力。UBT的当前实施例采用13 C-尿素并检测呼吸中的标记CO2。目前的UBT没有被广泛使用，因为它们昂贵并且在护理点不可用。我们建议开发一种简单的UBT，使用未标记的尿素作为底物，并在一个非常简单的设备中使用纳米传感器检测呼吸中的氨。我们的初步工作充分证明了我们的办法的可行性。我们的具体目标是：1）使用我们的传感器对患者进行UBT，以确定NH3; 2）开发便携式呼吸分析仪，用于检测H。幽门螺杆菌的基础上确定的NH3在人类呼吸。拟议的UBT将是简单的，廉价的，非侵入性的，可在护理点。因此，它将使一种简单的设备广泛用于幽门螺杆菌的诊断和治疗效果的监测。 公共卫生相关性：我们建议开发一种简单易用，价格低廉的护理点设备，以快速诊断幽门螺杆菌感染。这种微生物感染了大约50%的世界人口，并导致消化性溃疡。它也与胃癌的发展有关。该装置对公众健康的影响将是直接和重大的。