Protein-Glycan Interaction Resource of the CFG

描述(由申请人提供)：糖链微阵列的制备和可用性由NIGMS/NIH通过Glue-Grant支持的功能性糖链联盟(CFG)开发。这一资源使糖组学领域发生了革命性的变化，提供了500多种固定化多糖，用于一般筛选与识别糖决定因素的蛋白质(糖结合蛋白或GBP)的相互作用。这是一份申请，要求继续支持这一关键的“遗留”资源，因为在胶水赠款机制下成功开展了10年的工作后，CFG的资金已于2011年7月到期。在这里，我们建议将这一资源继续作为“CFG的蛋白质-葡聚糖相互作用资源”，其中CFG的两个关键核心，即核心D和核心H，将结合在一起，提供可供生物医学研究社区广泛使用的葡聚糖微阵列。目前，对利用这一资源感兴趣的调查人员将向CFG网站提交他们的请求，该网站将通过向CFG的生物信息学核心B提供其他遗留资金来维护。与目前一样，由该领域著名科学家组成的指导委员会将根据科学重要性和现有资源定期审查和批准/拒绝为调查人员提供资源。在获得批准后，研究人员将把样本送往埃默里大学医学院，在那里蛋白质-多糖相互作用资源将存放在目前由CFG的Core H使用并由Richard D.Cummings博士(PD/PI)协调的空间中。蛋白质-葡聚糖相互作用资源所使用的葡聚糖微阵列将由斯克里普斯研究所的James Paulson博士(PD/PI)协调的葡聚糖阵列生产资源提供，最初将是5.0版，但可以扩展，因为该领域的研究人员可以将多糖贡献给该计划。将在埃默里进行葡聚糖微阵列分析，并将完成的结果提供给调查人员和CFG的遗留生物信息学，以供公开张贴和管理。该计划将确保在糖链微阵列数据的准备、利用和管理方面达到最高水平的完整性和系统的卓越，并向广大研究社区提供这一宝贵的资源。

DESCRIPTION (provided by applicant): The preparation and availability of glycan microarrays was developed by the NIGMS/NIH through a Glue-Grant-supported Consortium for Functional Glycomics (CFG). This resource has revolutionized the glycomics field through making available a rich assortment of over 500 immobilized glycans for general screening for interactions with proteins that recognize glycan determinants (glycan-binding proteins or GBPs). This is an application for continued support of this key "legacy" resource due to the expiration of funding for the CFG in July 2011, following successful work for 10 years under the Glue Grant mechanism. Here we propose to continue this resource as the "Protein-Glycan Interaction Resource of the CFG", in which two of the key cores of the CFG, Core D and Core H, will be combined to make available glycan microarrays for wide use by biomedical research community. As currently, investigators interested in using this resource will submit their request to the CFG Website that will be maintained through other legacy funding to the Bioinformatics Core B of the CFG. As currently, a Steering Committee of eminent scientists in the field will periodically review and approve/deny resources for investigators based on the scientific importance and resources available. Following approval, investigators will send samples to Emory University School of Medicine where the Protein-Glycan Interaction Resource will be housed in space currently used by Core H of the CFG and coordinated by Dr. Richard D. Cummings (PD/PI). The glycan microarrays used by the Protein-Glycan Interaction Resource will be provided by the Glycan Array Production Resource coordinated by Dr. James Paulson (PD/PI) at The Scripps Research Institute, and will initially be Version 5.0, but can be expanded as glycans may be contributed to the program by investigators in the field. Glycan microarray analyses will be conducted at Emory and completed results made available to the investigators and to the legacy bioinformatics of the CFG for public posting and curation. This program will ensure the highest level of integrity and systematic excellence in the preparation, utilization, and management of glycan microarray data, and make available to the broad research community this valuable resource.