Members of the YjgF superfamily neutralize endogenous metabolic stressors.

YjgF 超家族的成员可以中和内源性代谢应激源。

基本信息

  • 批准号:
    8320101
  • 负责人:
  • 金额:
    $ 6.12万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2011
  • 资助国家:
    美国
  • 起止时间:
    2011-09-01 至 2012-12-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): A fundamental feature of a living system is an integrated network of biochemical pathways that can respond to stresses applied by the environment. Microbes, particularly those with a genetic system, provide a technically amenable system to characterize metabolic processes and stress responses. Metabolic strategies are conserved across biology, and insights obtained from microbial systems will contribute to our understanding of general metabolic paradigms. The long-term goal of my research is to contribute to the understanding of metabolic components and the processes they participate in. A rigorous understanding of metabolic processes is critical in efforts to predict the response of cells to environmental change, efforts to develop metabolic modeling strategies and efforts targeting metabolism for rational drug design and/or production of small molecules, to name a few. The goal of the work herein is to characterize a metabolic stress that results from reactive metabolites generated during growth, and to understand the family of proteins that neutralize this stress. This study focuses on a bacterial protein (YjgF) that is a member of highly conserved protein family that neutralizes reactive nitrogen species (e.g. enamines). In the current proposal, we will i) identify the stressors neutralized by the YjgF protein, ii) define the molecular consequences of not neutralizing this metabolic stress and iii) explore the relationship between sequence and functional divergence in the YjgF family. The goals of this proposal will be accomplished through the use of modern chemical, biochemical, biophysical, molecular, genetic and bioinformatic techniques. The work proposed here is motivated by our desire to understand the metabolic stress generated during growth by the production of reactive metabolites that can damage cellular components if they are not neutralized.
描述(由申请人提供):生命系统的一个基本特征是生化途径的综合网络,可以对环境施加的压力做出反应。微生物,特别是那些具有遗传系统的微生物,提供了一个技术上可适应的系统来表征代谢过程和应激反应。代谢策略在整个生物学中是保守的,从微生物系统中获得的见解将有助于我们对一般代谢范式的理解。我研究的长期目标是有助于理解代谢成分及其参与的过程。严格理解代谢过程对于预测细胞对环境变化的反应,开发代谢建模策略以及针对合理药物设计和/或小分子生产的代谢工作至关重要,仅举几例。本文的目标是描述生长过程中产生的反应性代谢物导致的代谢应激,并了解中和这种应激的蛋白质家族。本研究的重点是一种细菌蛋白(YjgF),它是高度保守的蛋白家族的成员,可以中和活性氮(如胺)。在目前的提案中,我们将i)确定被YjgF蛋白中和的应激源,ii)定义不中和这种代谢应激的分子后果,iii)探索YjgF家族序列和功能分化之间的关系。本建议的目标将通过使用现代化学、生物化学、生物物理、分子、遗传和生物信息学技术来实现。这里提出的工作的动机是我们希望了解生长过程中产生的代谢应激,这些代谢应激是由活性代谢物产生的,如果它们不被中和,就会损害细胞成分。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Diana M. Downs其他文献

Thiamin biosynthesis in prokaryotes
  • DOI:
    10.1007/s002030050713
  • 发表时间:
    1999-04-01
  • 期刊:
  • 影响因子:
    2.600
  • 作者:
    T. P. Begley;Diana M. Downs;Steven E. Ealick;Fred W. McLafferty;Adolphus P. G. M. Van Loon;Sean Taylor;Nino Campobasso;Hsiu-Ju Chiu;Cynthia Kinsland;Jason J. Reddick;Jun Xi
  • 通讯作者:
    Jun Xi
Biochemical and structural characterization of a reactive intermediate deaminase A homolog from Streptococcus sanguinis
来自血链球菌的一种反应性中间脱氨酶 A 同源物的生化和结构表征
  • DOI:
    10.1038/s41598-025-05264-x
  • 发表时间:
    2025-07-01
  • 期刊:
  • 影响因子:
    3.900
  • 作者:
    Alexa B. Benedict;Adreana I. Aquino;Brandi A. Buckner;Swarmistha Devi Aribam;Chhandosee Ganguly;Leonard M. Thomas;Philip Bourne;Rakhi Rajan;Diana M. Downs;Vijayakumar Somalinga
  • 通讯作者:
    Vijayakumar Somalinga
Purine limitation prevents the exogenous pyridoxal 5′-phosphate accumulation of emSalmonella enterica yggS/em mutants
嘌呤限制阻止了肠沙门氏菌 yggS 外源性吡哆醛 5′-磷酸的积累
  • DOI:
    10.1128/spectrum.02075-24
  • 发表时间:
    2024-10-30
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Kailey S. Ezekiel;Diana M. Downs
  • 通讯作者:
    Diana M. Downs

Diana M. Downs的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Diana M. Downs', 18)}}的其他基金

The RidA examine deaminase links metabolism with virulence in Campylobacter jejuni
RidA 研究脱氨酶将空肠弯曲杆菌代谢与毒力联系起来
  • 批准号:
    10204983
  • 财政年份:
    2020
  • 资助金额:
    $ 6.12万
  • 项目类别:
The RidA examine deaminase links metabolism with virulence in Campylobacter jejuni
RidA 研究脱氨酶将空肠弯曲杆菌代谢与毒力联系起来
  • 批准号:
    10039719
  • 财政年份:
    2020
  • 资助金额:
    $ 6.12万
  • 项目类别:
Rid family members neutralize endogenous metabolic stressors
Rid 家族成员中和内源性代谢应激源
  • 批准号:
    10469647
  • 财政年份:
    2011
  • 资助金额:
    $ 6.12万
  • 项目类别:
Rid family members neutralize endogenous metabolic stressors
Rid 家族成员中和内源性代谢应激源
  • 批准号:
    10673400
  • 财政年份:
    2011
  • 资助金额:
    $ 6.12万
  • 项目类别:
Rid family members neutralize endogenous metabolic stressors
Rid 家族成员中和内源性代谢应激源
  • 批准号:
    10683288
  • 财政年份:
    2011
  • 资助金额:
    $ 6.12万
  • 项目类别:
Rid family members neutralize endogenous metabolic stressors
Rid 家族成员中和内源性代谢应激源
  • 批准号:
    10260571
  • 财政年份:
    2011
  • 资助金额:
    $ 6.12万
  • 项目类别:
Members of the YjgF superfamily neutralize endogenous metabolic stressors.
YjgF 超家族的成员可以中和内源性代谢应激源。
  • 批准号:
    8186208
  • 财政年份:
    2011
  • 资助金额:
    $ 6.12万
  • 项目类别:
Rid family members neutralize endogenous metabolic stressors
Rid 家族成员中和内源性代谢应激源
  • 批准号:
    9323487
  • 财政年份:
    2011
  • 资助金额:
    $ 6.12万
  • 项目类别:
Diversity Enhancement Supplement
多样性增强补充
  • 批准号:
    10328806
  • 财政年份:
    2011
  • 资助金额:
    $ 6.12万
  • 项目类别:
Rid family members neutralize endogenous metabolic stressors
Rid 家族成员中和内源性代谢应激源
  • 批准号:
    10467136
  • 财政年份:
    2011
  • 资助金额:
    $ 6.12万
  • 项目类别:

相似海外基金

Quantification of Neurovasculature Changes in a Post-Hemorrhagic Stroke Animal-Model
出血性中风后动物模型中神经血管变化的量化
  • 批准号:
    495434
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
Small animal model for evaluating the impacts of cleft lip repairing scar on craniofacial growth and development
评价唇裂修复疤痕对颅面生长发育影响的小动物模型
  • 批准号:
    10642519
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
Bioactive Injectable Cell Scaffold for Meniscus Injury Repair in a Large Animal Model
用于大型动物模型半月板损伤修复的生物活性可注射细胞支架
  • 批准号:
    10586596
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
A Comparison of Treatment Strategies for Recovery of Swallow and Swallow-Respiratory Coupling Following a Prolonged Liquid Diet in a Young Animal Model
幼年动物模型中长期流质饮食后吞咽恢复和吞咽呼吸耦合治疗策略的比较
  • 批准号:
    10590479
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
Diurnal grass rats as a novel animal model of seasonal affective disorder
昼夜草鼠作为季节性情感障碍的新型动物模型
  • 批准号:
    23K06011
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Longitudinal Ocular Changes in Naturally Occurring Glaucoma Animal Model
自然发生的青光眼动物模型的纵向眼部变化
  • 批准号:
    10682117
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
A whole animal model for investigation of ingested nanoplastic mixtures and effects on genomic integrity and health
用于研究摄入的纳米塑料混合物及其对基因组完整性和健康影响的整体动物模型
  • 批准号:
    10708517
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
A Novel Large Animal Model for Studying the Developmental Potential and Function of LGR5 Stem Cells in Vivo and in Vitro
用于研究 LGR5 干细胞体内外发育潜力和功能的新型大型动物模型
  • 批准号:
    10575566
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
Elucidating the pathogenesis of a novel animal model mimicking chronic entrapment neuropathy
阐明模拟慢性卡压性神经病的新型动物模型的发病机制
  • 批准号:
    23K15696
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
The effect of anti-oxidant on swallowing function in an animal model of dysphagia
抗氧化剂对吞咽困难动物模型吞咽功能的影响
  • 批准号:
    23K15867
  • 财政年份:
    2023
  • 资助金额:
    $ 6.12万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了