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Metabolic Studies Core

代谢研究核心

基本信息

批准号：
8132706
负责人：
MICHAEL D. JENSEN
金额：
$ 22.98万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-04-01 至 2016-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8132706
关键词：
Affect Aging Alcohols Animals Biological Assay Biological Markers Body Composition Bone Density Budgets Burn injury Calibration Charge Cities Clinical Clinical Sciences Collaborations Devices Dual-Energy X-Ray Absorptiometry Energy Metabolism Epidemiology Equipment Experimental Designs Faculty Gases Healthcare Housing Human Institutes Institution Investigation Kinesiology Laboratories Link Lipids Mass Spectrum Analysis Measurement Measures Methods Minnesota Nutrient Obesity Participant Physical activity Physiology Positioning Attribute Prodigy Proteomics Published Comment Quality Control Research Research Design Research Personnel Resolution Resources Sampling Scanning Screening procedure Services Site System Techniques Technology Testing Translational Research Twin Multiple Birth Universities Wages Work base beta-Hydroxybutyrate cost high throughput screening instrument interest lipid metabolism meetings metabolic abnormality assessment metabolomics novel professor programs response

项目摘要

We have adapted the core functions to the needs of the users as follows: 1. We removed the animal physiology unit as part of our MNOC offering - partly because Dr. Novak took a faculty position at Kent State and also in response to comments from our last renewal. 2. We thoroughly tested the accuracy and precision of the Parvo Medics indirect calorimeters that we purchased to replace the failing Oxymax and SensorMedics DeltaTrack systems. We evaluated a number of commercially available indirect calorimeters because SensorMedics is no longer supporting the DeltaTrack system. We completed human studies to cross-calibrate the devices and devised the implementation system for alcohol burn calibration. Although the ParvoMedics was the best ofthe available options, it is not as reliable as DeltaTrack. We have therefore worked to make investigators aware of the quality differences so that they can adjust their experimental designs as needed (power calculations are based upon precision ofthe measurements). 3. We have developed novel in-house LC\MS\MS assays to support University of Minnesota and Mayo investigators studying intracellular lipid metabolism. 4. We have moved to a per sample charge system for mass spectrometry analysis done in the Mayo Metabolomics Core (formerly the Biomedical Mass Spectrometry Core). We previously purchased supplies and paid the salary of one ofthe technicians in the Biomedical Mass Spectrometry Core/Metabolomics Core in exchange for having investigator's samples analyzed at no charge. We will now use a portion of our budget to subsidize the cost of the samples for MNOC investigators. 5. We have transferred the physical activity assessment components of the Metabolic Studies Core to the Epidemiology Core because the application of this technology seemed most appropriate to that core function. That core is directed by Dr. Robert Jeffery, and Dr. James Levine will now serve as a director for a subcore within the epidemiology core. 6. We have incorporated the Metabolomics Discovery function of the University of Minnesota Center for Mass Spectrometry and Proteomics (CMSP) into the Metabolic Studies Core. This core is directed by Dr. Gary Nelsestuen, who will now serve as an associate director of this core. Since 2008, the CMSP has greatly expanded its capability in metabolite discovery research and will fulfill this new service to the program. 7. We have purchased a new Roche Integra 400 Plus multichannel analyzer to replace our aging (and no longer serviceable) Cobas centrifugal analyzer. This instrument allows us to perform high throughput assays for lipids, FFA, beta- hydroxybutyrate, etc. for investigators who need large numbers of samples analyzed. 8. We replaced the older Mayo DXA instruments with two new GE Healthcare iDXAs. The IDXA employs high-resolution technology to perform bone density and total body composition testing analysis. Although our Prodigy DXA performed bone density measurements well, the IDXA is a far superior instrument for body composition analysis. The iDXA is the only high-resolution DXA available that can accommodate obese research participants up to 450 lbs. In 2008 we performed a cross-calibration study between the two older, failing DXA scanners and the new IDXA. This allowed us provide conversion formulas to investigators whose studies were affected by the change. Different DXA instruments provide slightly different values, and thus changing scanners in the middle of a study, whether it is longitudinal or crosssectional, can be problematic. By centralizing our body composition resources and having a dedicated staff, we can provide the best service for large numbers of users. 9. The GE DXA Prodigy Scanner at the Twin Cities campus has been replaced by a GE Healthcare Lunar iDXA purchased by the Clinical and Translational Science Institute (CTSI). Dr. Donald Dengel (Associate Professor - Kinesiology) is the director of the Laboratory of Integrative Human Physiology where the iDXA is located. He and Dr. Jensen cooperate to assure similar quality control standards between the two sites. Because of the mechanism via which this IDXA was purchased, the CTSI now charges MNOC investigators for these scans. The Metabolic Studies Core will subsidize this expense for MNOC investigators.

我们根据用户的需求调整了核心功能，如下所示： 1.我们删除了动物生理学单位作为我们的MNOC提供的一部分-部分原因是诺瓦克博士在肯特州立大学担任教职，也是为了回应我们上次续约的意见。 2.我们彻底测试了Parvo Medics间接热量计的准确性和精度，我们购买了该热量计以取代失败的Oxymax和SensorMedics DeltaTrack系统。由于SensorMedics不再支持DeltaTrack系统，我们评估了许多市售间接热量计。我们完成了人体研究，交叉校准的设备，并设计了酒精烧伤校准的实施系统。虽然ParvoMedics是最好的可用它不像DeltaTrack那样可靠。因此，我们致力于让研究人员了解质量差异，以便他们可以根据需要调整实验设计（功效计算基于测量的精度）。 3.我们开发了新型的内部LC\MS\MS分析，以支持明尼苏达大学和马约研究人员研究细胞内脂质代谢。 4.我们已经转移到马约代谢组学核心（以前的生物医学质谱核心）中进行质谱分析的每个样品充电系统。我们以前购买了物资，并支付了生物医学质谱核心/代谢组学核心的一名技术人员的工资，以换取研究人员的样品免费分析。我们现在将使用一部分我们的预算来补贴MNOC调查员的样本费用。 5.我们已将代谢研究核心的体力活动评估组件转移到流行病学核心，因为该技术的应用似乎最适合该核心功能。该核心由Robert Jeffery博士指导，James Levine博士现在将担任流行病学核心内的一个子核心的主任。 6.我们已将明尼苏达大学质谱和蛋白质组学中心（CMSP）的代谢组学发现功能纳入代谢研究核心。该核心由加里内尔塞斯图恩博士指导，他现在将担任该核心的副主任。自2008年以来，CMSP大大扩展了其在代谢物发现研究方面的能力，并将为该计划提供这项新服务。 7.我们购买了一台新的Roche Integra 400 Plus多通道分析仪，以替换我们老化（不再可用）的Cobas离心分析仪。该仪器使我们能够为需要分析大量样本的研究人员进行脂质、FFA、β-羟基丁酸等的高通量测定。 8.我们用两台新的GE Healthcare iDXA替换了旧的马约DXA仪器。IDXA采用高分辨率技术进行骨密度和全身成分测试分析。虽然我们的Prodigy DXA能很好地进行骨密度测量，但IDXA是一种远优于身体成分分析的上级仪器。iDXA是唯一一款可容纳体重不超过450磅的肥胖研究参与者的高分辨率DXA。2008年，我们在这两者之间进行了交叉校准研究。旧的，失败的DXA扫描仪和新的IDXA。这使我们能够向研究受到变化影响的研究人员提供转换公式。不同的DXA仪器提供的值略有不同，因此在研究过程中更换扫描仪，无论是纵向还是横截面，都可能存在问题。通过集中我们的身体成分资源并拥有专门的工作人员，我们可以为大量用户提供最好的服务。 9.双城校区的GE DXA Prodigy扫描仪已被临床和转化科学研究所（CTSI）购买的GE Healthcare Lunar iDXA取代。Donald Dengel博士（运动机能学副教授）是iDXA所在的综合人体生理学实验室的主任。他和詹森博士合作，以确保两个研究中心的质量控制标准相似。由于购买IDXA的机制，CTSI现在向MNOC调查人员收取这些扫描的费用。代谢研究中心将为MNOC研究人员补贴这笔费用。