Metabolic Studies Core

代谢研究核心

• 批准号: 8132706
• 负责人: MICHAEL D. JENSEN
• 金额: $ 22.98万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8132706
• 关键词: Affect; Aging; Alcohols; Animals; Biological Assay; Biological Markers; Body Composition; Bone Density; Budgets; Burn injury; Calibration; Charge; Cities; Clinical; Clinical Sciences; Collaborations; Devices; Dual-Energy X-Ray Absorptiometry; Energy Metabolism; Epidemiology; Equipment; Experimental Designs; Faculty; Gases; Healthcare; Housing; Human; Institutes; Institution; Investigation; Kinesiology; Laboratories; Link; Lipids; Mass Spectrum Analysis; Measurement; Measures; Methods; Minnesota; Nutrient; Obesity; Participant; Physical activity; Physiology; Positioning Attribute; Prodigy; Proteomics; Published Comment; Quality Control; Research; Research Design; Research Personnel; Resolution; Resources; Sampling; Scanning; Screening procedure; Services; Site; System; Techniques; Technology; Testing; Translational Research; Twin Multiple Birth; Universities; Wages; Work; base; beta-Hydroxybutyrate; cost; high throughput screening; instrument; interest; lipid metabolism; meetings; metabolic abnormality assessment; metabolomics; novel; professor; programs; response

We have adapted the core functions to the needs of the users as follows: 1. We removed the animal physiology unit as part of our MNOC offering - partly because Dr. Novak took a faculty position at Kent State and also in response to comments from our last renewal. 2. We thoroughly tested the accuracy and precision of the Parvo Medics indirect calorimeters that we purchased to replace the failing Oxymax and SensorMedics DeltaTrack systems. We evaluated a number of commercially available indirect calorimeters because SensorMedics is no longer supporting the DeltaTrack system. We completed human studies to cross-calibrate the devices and devised the implementation system for alcohol burn calibration. Although the ParvoMedics was the best ofthe available options, it is not as reliable as DeltaTrack. We have therefore worked to make investigators aware of the quality differences so that they can adjust their experimental designs as needed (power calculations are based upon precision ofthe measurements). 3. We have developed novel in-house LC\MS\MS assays to support University of Minnesota and Mayo investigators studying intracellular lipid metabolism. 4. We have moved to a per sample charge system for mass spectrometry analysis done in the Mayo Metabolomics Core (formerly the Biomedical Mass Spectrometry Core). We previously purchased supplies and paid the salary of one ofthe technicians in the Biomedical Mass Spectrometry Core/Metabolomics Core in exchange for having investigator's samples analyzed at no charge. We will now use a portion of our budget to subsidize the cost of the samples for MNOC investigators. 5. We have transferred the physical activity assessment components of the Metabolic Studies Core to the Epidemiology Core because the application of this technology seemed most appropriate to that core function. That core is directed by Dr. Robert Jeffery, and Dr. James Levine will now serve as a director for a subcore within the epidemiology core. 6. We have incorporated the Metabolomics Discovery function of the University of Minnesota Center for Mass Spectrometry and Proteomics (CMSP) into the Metabolic Studies Core. This core is directed by Dr. Gary Nelsestuen, who will now serve as an associate director of this core. Since 2008, the CMSP has greatly expanded its capability in metabolite discovery research and will fulfill this new service to the program. 7. We have purchased a new Roche Integra 400 Plus multichannel analyzer to replace our aging (and no longer serviceable) Cobas centrifugal analyzer. This instrument allows us to perform high throughput assays for lipids, FFA, beta- hydroxybutyrate, etc. for investigators who need large numbers of samples analyzed. 8. We replaced the older Mayo DXA instruments with two new GE Healthcare iDXAs. The IDXA employs high-resolution technology to perform bone density and total body composition testing analysis. Although our Prodigy DXA performed bone density measurements well, the IDXA is a far superior instrument for body composition analysis. The iDXA is the only high-resolution DXA available that can accommodate obese research participants up to 450 lbs. In 2008 we performed a cross-calibration study between the two older, failing DXA scanners and the new IDXA. This allowed us provide conversion formulas to investigators whose studies were affected by the change. Different DXA instruments provide slightly different values, and thus changing scanners in the middle of a study, whether it is longitudinal or crosssectional, can be problematic. By centralizing our body composition resources and having a dedicated staff, we can provide the best service for large numbers of users. 9. The GE DXA Prodigy Scanner at the Twin Cities campus has been replaced by a GE Healthcare Lunar iDXA purchased by the Clinical and Translational Science Institute (CTSI). Dr. Donald Dengel (Associate Professor - Kinesiology) is the director of the Laboratory of Integrative Human Physiology where the iDXA is located. He and Dr. Jensen cooperate to assure similar quality control standards between the two sites. Because of the mechanism via which this IDXA was purchased, the CTSI now charges MNOC investigators for these scans. The Metabolic Studies Core will subsidize this expense for MNOC investigators.

我们已将核心功能调整为用户的需求，如下所示： 1。作为MNOC产品的一部分，我们删除了动物生理部门 - 部分是因为Novak博士在肯特州担任了教职员工职位，并回应了我们上次续签的评论。 2。我们彻底测试了我们购买的parvo Medics间接热量表的准确性和精度，以取代失败的Oxymax和Sensormedics Deltatrack Systems。我们评估了许多市售的间接热量表，因为Sensormedics不再支持Deltatrack系统。我们完成了人类研究以对设备进行跨校准，并设计了酒精燃烧校准的实施系统。虽然Parvomedics是可用的最好的 选项，它不像Deltatrack那样可靠。因此，我们努力使研究人员意识到质量差异，以便他们可以根据需要调整实验设计（功率计算基于测量的精确度）。 3。我们已经开发了新型的内部LC \ MS \ MS分析，以支持明尼苏达大学和研究细胞内脂质代谢的梅奥大学研究人员。 4。我们已移至每个样品电荷系统，以在蛋黄酱代谢组核心（以前是生物医学质谱核心）中进行的质谱分析。我们以前购买了耗材，并在生物医学质谱核心/代谢组学核心中支付了一位技术人员的薪水，以换取未负责调查员的样品。现在我们将使用一部分 我们的预算为MNOC调查人员提供了样品的费用。 5。我们将代谢研究核心的体育活动评估组成部分转移到了流行病学核心，因为该技术的应用似乎最适合该核心功能。该核心由罗伯特·杰弗里（Robert Jeffery）博士指导，詹姆斯·莱文（James Levine）博士现在将担任流行病学核心中的子核心主任。 6。我们将明尼苏达大学质谱与蛋白质组学中心（CMSP）的代谢组发现功能纳入了代谢研究核心。该核心由加里·尼尔斯图恩（Gary Nelsestuen）博士指导，他现在将担任该核心的副主任。自2008年以来，CMSP大大扩展了其在代谢发现研究中的能力，并将为该计划提供这项新服务。 7。我们购买了新的Roche Integra 400加多通道分析仪，以取代我们的衰老（不再有用）Cobas离心分析仪。该仪器允许我们对需要大量样品进行分析的研究人员进行脂质，FFA，β-羟基苯二甲酸酯等的高通量测定。 8。我们用两个新的GE Healthcare IDXA代替了较旧的Mayo DXA仪器。 IDXA采用高分辨率技术来执行骨密度和总体组成测试分析。尽管我们的天才DXA执行了骨密度测量值，但IDXA是人体组成分析的优越仪器。 IDXA是唯一可容纳肥胖研究参与者最多450磅的高分辨率DXA。在2008年，我们进行了两者之间的跨校准研究 年龄较大的DXA扫描仪和新的IDXA。这使我们向研究人员提供了受到变化影响的研究人员的转化公式。不同的DXA仪器提供略有不同的值，因此在研究中间会改变扫描仪，无论是纵向还是横截面，都可能是有问题的。通过集中我们的身体组成资源并拥有专门的员工，我们可以为大量用户提供最佳服务。 9。双城校园的GE DXA神童扫描仪已被临床和转化科学研究所（CTSI）购买的GE Healthcare Lunar IDXA取代。 Donald Dengel博士（运动机能学副教授）是IDXA所在的综合人类生理学实验室主任。他和詹森博士合作确保两个站点之间的类似质量控制标准。 由于购买了此IDXA的机制，CTSI现在向MNOC调查人员收取这些扫描费用。代谢研究核心将为MNOC研究人员提供这笔费用。