Metabolic Studies Core

代谢研究核心

• 批准号: 8132706
• 负责人: MICHAEL D. JENSEN
• 金额: $ 22.98万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8132706
• 关键词: Affect; Aging; Alcohols; Animals; Biological Assay; Biological Markers; Body Composition; Bone Density; Budgets; Burn injury; Calibration; Charge; Cities; Clinical; Clinical Sciences; Collaborations; Devices; Dual-Energy X-Ray Absorptiometry; Energy Metabolism; Epidemiology; Equipment; Experimental Designs; Faculty; Gases; Healthcare; Housing; Human; Institutes; Institution; Investigation; Kinesiology; Laboratories; Link; Lipids; Mass Spectrum Analysis; Measurement; Measures; Methods; Minnesota; Nutrient; Obesity; Participant; Physical activity; Physiology; Positioning Attribute; Prodigy; Proteomics; Published Comment; Quality Control; Research; Research Design; Research Personnel; Resolution; Resources; Sampling; Scanning; Screening procedure; Services; Site; System; Techniques; Technology; Testing; Translational Research; Twin Multiple Birth; Universities; Wages; Work; base; beta-Hydroxybutyrate; cost; high throughput screening; instrument; interest; lipid metabolism; meetings; metabolic abnormality assessment; metabolomics; novel; professor; programs; response

We have adapted the core functions to the needs of the users as follows: 1. We removed the animal physiology unit as part of our MNOC offering - partly because Dr. Novak took a faculty position at Kent State and also in response to comments from our last renewal. 2. We thoroughly tested the accuracy and precision of the Parvo Medics indirect calorimeters that we purchased to replace the failing Oxymax and SensorMedics DeltaTrack systems. We evaluated a number of commercially available indirect calorimeters because SensorMedics is no longer supporting the DeltaTrack system. We completed human studies to cross-calibrate the devices and devised the implementation system for alcohol burn calibration. Although the ParvoMedics was the best ofthe available options, it is not as reliable as DeltaTrack. We have therefore worked to make investigators aware of the quality differences so that they can adjust their experimental designs as needed (power calculations are based upon precision ofthe measurements). 3. We have developed novel in-house LC\MS\MS assays to support University of Minnesota and Mayo investigators studying intracellular lipid metabolism. 4. We have moved to a per sample charge system for mass spectrometry analysis done in the Mayo Metabolomics Core (formerly the Biomedical Mass Spectrometry Core). We previously purchased supplies and paid the salary of one ofthe technicians in the Biomedical Mass Spectrometry Core/Metabolomics Core in exchange for having investigator's samples analyzed at no charge. We will now use a portion of our budget to subsidize the cost of the samples for MNOC investigators. 5. We have transferred the physical activity assessment components of the Metabolic Studies Core to the Epidemiology Core because the application of this technology seemed most appropriate to that core function. That core is directed by Dr. Robert Jeffery, and Dr. James Levine will now serve as a director for a subcore within the epidemiology core. 6. We have incorporated the Metabolomics Discovery function of the University of Minnesota Center for Mass Spectrometry and Proteomics (CMSP) into the Metabolic Studies Core. This core is directed by Dr. Gary Nelsestuen, who will now serve as an associate director of this core. Since 2008, the CMSP has greatly expanded its capability in metabolite discovery research and will fulfill this new service to the program. 7. We have purchased a new Roche Integra 400 Plus multichannel analyzer to replace our aging (and no longer serviceable) Cobas centrifugal analyzer. This instrument allows us to perform high throughput assays for lipids, FFA, beta- hydroxybutyrate, etc. for investigators who need large numbers of samples analyzed. 8. We replaced the older Mayo DXA instruments with two new GE Healthcare iDXAs. The IDXA employs high-resolution technology to perform bone density and total body composition testing analysis. Although our Prodigy DXA performed bone density measurements well, the IDXA is a far superior instrument for body composition analysis. The iDXA is the only high-resolution DXA available that can accommodate obese research participants up to 450 lbs. In 2008 we performed a cross-calibration study between the two older, failing DXA scanners and the new IDXA. This allowed us provide conversion formulas to investigators whose studies were affected by the change. Different DXA instruments provide slightly different values, and thus changing scanners in the middle of a study, whether it is longitudinal or crosssectional, can be problematic. By centralizing our body composition resources and having a dedicated staff, we can provide the best service for large numbers of users. 9. The GE DXA Prodigy Scanner at the Twin Cities campus has been replaced by a GE Healthcare Lunar iDXA purchased by the Clinical and Translational Science Institute (CTSI). Dr. Donald Dengel (Associate Professor - Kinesiology) is the director of the Laboratory of Integrative Human Physiology where the iDXA is located. He and Dr. Jensen cooperate to assure similar quality control standards between the two sites. Because of the mechanism via which this IDXA was purchased, the CTSI now charges MNOC investigators for these scans. The Metabolic Studies Core will subsidize this expense for MNOC investigators.

我们根据用户的需求对核心功能进行了如下调整： 1.作为我们MNOC课程的一部分，我们删除了动物生理学单元--部分原因是诺瓦克博士在肯特州立大学担任教职，也是为了回应我们上一次续签的评论。 2.我们彻底测试了我们购买的Parvo Meds间接量热仪的准确性和精密度，这些热计是为了更换故障的Oxymax和SensorMedics DeltaTrack系统而购买的。由于SensorMedics不再支持DeltaTrack系统，我们评估了许多商业上可用的间接量热计。我们完成了交叉校准设备的人体实验，并设计了酒精烧伤校准的实施系统。尽管ParvoMedics是现有的最好的 选项，它不如DeltaTrack可靠。因此，我们努力让研究人员意识到质量差异，以便他们可以根据需要调整他们的实验设计(功率计算基于测量的精度)。 3.我们开发了新的内部LC\MS\MS分析方法，以支持明尼苏达大学和梅奥大学研究细胞内脂质代谢的研究人员。 4.我们已转向按样本收费系统，用于在Mayo代谢组学核心(以前的生物医学质谱学核心)进行的质谱分析。我们之前购买了用品，并支付了生物医学质谱学核心/代谢组学核心的一名技术人员的工资，以换取免费分析研究人员的样本。我们现在将使用一部分 我们的预算用于补贴MNOC调查人员的样本费用。 5.我们已将新陈代谢研究核心的体力活动评估部分转至流行病学核心，因为这项技术的应用似乎最适合该核心职能。该核心由罗伯特·杰弗里博士领导，詹姆斯·莱文博士现在将担任流行病学核心内的一个分核心的主任。 6.我们已将明尼苏达大学质谱学和蛋白质组学中心(CMSP)的代谢组学发现功能纳入代谢研究核心。这个核心由加里·内尔斯图恩博士指导，他现在将担任这个核心的副主任。自2008年以来，CMSP大大扩展了其在代谢物发现研究方面的能力，并将为该计划提供这项新服务。 7.我们购买了一台新的罗氏Integra 400 Plus多道分析仪，以更换老化(不再使用)的Cobas离心式分析仪。该仪器使我们能够为需要分析大量样品的研究人员进行高通量的血脂、FFA、β-羟基丁酸酯等分析。 8.我们用两台新的GE Healthcare iDXA更换了较旧的Mayo DXA仪器。IDXA采用高分辨率技术进行骨密度和全身成分测试分析。尽管我们的奇才DXA进行了很好的骨密度测量，但iDXA是一种更好的身体成分分析仪器。IDXA是目前市面上唯一可以容纳体重达450磅的肥胖研究参与者的高分辨率DXA。2008年，我们在两者之间进行了一项交叉校准研究 旧的、故障的DXA扫描仪和新的iDXA。这使我们能够向研究受到变化影响的研究人员提供转换公式。不同的DXA仪器提供的值略有不同，因此在研究过程中更换扫描仪，无论是纵向还是横断面，都可能是有问题的。通过集中身体成分资源和拥有敬业的员工，我们可以为大量用户提供最好的服务。 9.双城校区的GE DXA Prodigy扫描仪已被临床和翻译科学研究所(CTSI)购买的GE Healthcare LUNAR iDXA取代。唐纳德·登格尔博士(运动学副教授)是iDXA所在的综合人体生理学实验室的主任。他和詹森博士合作，确保两个网站之间达到类似的质量控制标准。 由于购买这款iDXA的机制，CTSI现在向MNOC调查人员收取这些扫描费用。新陈代谢研究核心将补贴MNOC调查人员的这笔费用。