JHU Center for Neuroscience Research

约翰霍普金斯大学神经科学研究中心

• 批准号: 8214191
• 负责人: DWIGHT E BERGLES
• 金额: $ 27.57万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8214191
• 关键词: Air; Area; Basic Science; Calendar; Cells; Characteristics; Clinical Sciences; Custom; Data; Devices; Dyes; Educational process of instructing; Electrophysiology (science); Elevator; Ensure; Equipment; Financial compensation; Floor; Funding; Goals; Grant; Housing; Image; Image Analysis; Immersion Investigative Technique; Institution; Ions; Label; Laboratories; Lasers; Life; Medical; Microscope; Mitochondria; Monitor; Motor; Movement; National Institute of Neurological Disorders and Stroke; Neurosciences; Neurosciences Research; Oils; Online Systems; Optics; Output; Physiologic pulse; Positioning Attribute; Preparation; Process; Protein Dynamics; Proteins; Research; Research Personnel; Resolution; Sampling; Schedule; Site; Speed; System; Three-Dimensional Image; Time; Tissues; Training; Vestibule; Water; Wood material; Work; cell injury; cell motility; design; fluorophore; image processing; in vivo; instrumentation; member; millisecond; operation; photolysis; pre-clinical; protein protein interaction; square foot; two-photon; vibration

C1A. MULTIPHOTON IMAGING CORE C1 A l ¿ Establishment and setup of the Core: The Multiphoton Imaging (MPI) Core JHU became fully operational in November 2006 with support from this grant and funds provided by the institution. During the first year of funding, design and renovation of dedicated space suitable for imaging was completed, instrumentation was purchased and installed, and a fulltime technician was hired and trained. Dr. Bergles serves as the Director of this Core and is assisted by Dr. Pucak, who serves as Core Manager of the facility. The goals of this Core are to provide access to cuttingedge instrumentation for: 1) analyzing protein localization, protein dynamics, and protein-protein interactions with high resolution; 2) performing time-lapse imaging of multiple fluorophores in living cells and tissues; and 3) combining high-resolution imaging of fluorescently tagged proteins or ion indicator dyes with electrophysiological monitoring of electrical activity. The MPI Core was originally located on the 10th floor of the Wood Basic Science building in the Basic Science research cluster on the JHU medical campus. With institutional support, this facility was relocated in September 2009 down the hall to newly renovated space on the 10"^ floor of the immediately adjacent Preclinical Teaching Building. This move increased the size of the facility 2-fold to ~500 sq. ft., which allowed reorganization of the microscope layout for more efficient use (necessitated after the installation of the second microscope), addition of a dedicated preparation area, and expansion of the image processing suite. The Core is currently divided into one large room (~350 sq. ft.) that houses a vibration isolation table, two microscopes and associated equipment, and an outer vestibule (~150 sq. ft.) that is used as an office by the senior research technician responsible for day-to-day operation of the facility and as a site for analysis of imaging and electrophysiology data. The original instrumentation for this Core (Zeiss LSM 510 NLO Meta) was provided by funds from the JHU SOM, and all space for the Core was provided by the Department of Neuroscience. Scheduling for this facility is aided by an interactive web-based calendar accessible through the JHU Neuroscience portal. This Core is located on the same floor as the laboratories of Core members Drs. Bergles, Ginty, Huganir, Kolodkin (PI) and Sockanathan, and it is readily accessible by the other investigators listed in this proposal who have laboratories on other floors in the same building or in adjacent buildings. The Preclinical Teaching Building is sen/ed by two elevators and is connected by a covered bridge to other buildings at JHU SOM, providing convenient access to the three Cores for all NINDS investigators in clinical and basic science departments.

C1A多光子成像核心 C1 A l <$核心的建立和设置： JHU多光子成像（MPI）核心项目在该机构提供的赠款和资金的支持下于2006年11月开始全面运作。在供资的第一年，完成了适合成像的专用空间的设计和翻修，购买和安装了仪器，并雇用和培训了一名全职技术员。Bergles博士担任该核心的主任，并由Dr. 普卡克，谁担任该设施的核心经理。该核心的目标是提供尖端仪器的使用：1）以高分辨率分析蛋白质定位，蛋白质动力学和蛋白质-蛋白质相互作用; 2）对活细胞和组织中的多个荧光团进行延时成像; 3）将荧光标记蛋白质或离子指示染料的高分辨率成像与 电活动的电生理监测。MPI核心最初位于JHU医学校园基础科学研究集群中的Wood基础科学大楼的10楼。在机构的支持下，该设施于2009年9月搬迁到紧邻临床前教学楼10”^层的新装修空间。这一举动增加了规模的 设施2倍至约500平方米英尺，这允许重新组织显微镜布局以更有效地使用（在安装第二显微镜之后是必要的）、增加专用制备区域以及扩展图像处理套件。核心目前分为一个大房间（约350平方米）。（英尺）它容纳了一个隔振台，两个显微镜和相关设备，以及一个外部前庭（约150平方米）。（英尺）这是负责该设施日常运作的高级研究技术员的办公室，也是分析成像和电生理数据的场所。该核心的原始仪器（Zeiss LSM 510 NLO Meta）由JHU SOM的资金提供，核心的所有空间由神经科学系提供。该设施的日程安排由可通过JHU神经科学门户访问的交互式网络日历提供帮助。该核心与核心成员Bergles、Ginty、Huganir、Kolodkin（PI）和Sockanathan博士的实验室位于同一楼层，并且本提案中列出的其他研究者（在同一建筑物或相邻建筑物的其他楼层设有实验室）可以随时进入。临床前教学楼由两部电梯发送/艾德，并通过廊桥连接到JHU SOM的其他建筑物，为临床和基础科学部门的所有NINDS研究人员提供方便的三个核心。