LC-Orbitrap MS/MS System for shotgun Proteomics at Dartmouth

达特茅斯用于鸟枪蛋白质组学的 LC-Orbitrap MS/MS 系统

• 批准号: 8447223
• 负责人: Scott A. Gerber
• 金额: $ 60万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447223
• 关键词: Biomedical Research; Cell Cycle Regulation; Circadian Rhythms; Communities; Faculty; Funding; Future; Goals; Housing; Human; Malignant neoplasm of lung; Norris Cotton Cancer Center; Proteomics; Proteomics Shared Resource; Research; Research Project Grants; Secure; Shotguns; Signal Transduction; System; United States National Institutes of Health; Work; college; instrumentation; insulin signaling; mass spectrometer; medical schools; pathogen; research study

DESCRIPTION (provided by applicant): The objective of this proposal is to secure funding for a microcapillary LC-LTQ Orbitrap Elite mass spectrometer system to support shotgun proteomics-oriented, NIH-funded scientific goals of the greater Dartmouth biomedical research community. The users of the proposed system described in this application come from a broad range of departments and centers at Dartmouth College and Dartmouth Medical School, including the NCI-designated Norris Cotton Cancer Center (NCCC). Their projects represent a diverse continuum of research foci, from basic cellular mechanisms of cell cycle control and circadian rhythms to mechanisms of host-pathogen interactions and insulin signaling, to more translational projects aimed at understanding how cellular signaling is dysregulated in human lung cancers. Collectively, these research projects represent work and effort that has exceeded the capacity of the single LC-MS/MS platform that exists in all of Dartmouth for such experiments. Thus, the acquisition of a new mass spectrometer into the Dartmouth Proteomics Shared Resource is critically important to enable the completion of current, as well as future, NIH research goals of our faculty. The proposed instrumentation will be housed in our Proteomics Shared Resource, which is maintained by NCCC on behalf of the greater Dartmouth community.

描述（由申请人提供）：该提案的目的是为微毛细血管LC-LTQ Orbitrap精英质谱仪系统提供资金，以支持大达特茅斯生物医学研究社区的面向shot弹枪蛋白质组学，NIH资助的科学目标。该应用程序中描述的拟议系统的用户来自达特茅斯学院和达特茅斯医学院的各个部门和中心，包括NCI指定的诺里斯棉花癌中心（NCCC）。他们的项目代表了研究重点的多样化连续性，从细胞周期控制和昼夜节律的基本细胞机制到宿主 - 病原体相互作用和胰岛素信号传导的机制，再到更多旨在了解人类肺癌中细胞信号失调的转化项目。总的来说，这些研究项目代表了超出达特茅斯全部用于此类实验的单个LC-MS/MS平台的能力。因此，将新的质谱仪收购纳入达特茅斯蛋白质组学共享资源对于使我们学院的当前以及未来的NIH研究目标的完成至关重要。拟议的仪器将安置在我们的蛋白质组学共享资源中，该资源由NCCC代表大达特茅斯社区维护。