A request for funds to purchase an AB Sciex TripleTOF 5600 mass spectrometer

申请资金购买 AB Sciex TripleTOF 5600 质谱仪

• 批准号: 8447976
• 负责人: William Russell
• 金额: $ 60万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2014-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447976
• 关键词: 13 year old; Biological; Blood capillaries; Complex; Evaluation; Funding; Funding Applicant; Grant; Health; Housing; Human; Laboratories; Lipids; Liquid Chromatography; Maintenance; Mass Spectrum Analysis; Molecular; Outcome; Peptides; Proteins; Public Health; Pulsar; Research; Research Personnel; Research Project Grants; Resolution; Scientist; System; Texas; Training; United States National Institutes of Health; Universities; anticancer research; capillary; complex biological systems; drug discovery; instrument; instrumentation; mass spectrometer; nano; operation; structural biology

DESCRIPTION (provided by applicant): Funds are requested to purchase an AB Sciex TripleTOF" 5600 mass spectrometer equipped with capillary/nano liquid chromatography. The instrument will be primarily used to facilitate the research of 14 NIH funded investigators within the Texas A&M University (TAMU) system. Scientists at the forefront of exciting efforts including cancer research, human health, structural biology, and drug discovery al require the ability to accurately identify, and characterize important molecular species and determine specific interactions between complexes of molecules. The 5600 will provide NIH funded researchers at TAMU significantly better capabilities in the analysis of complex biological systems, specifically proteins, modified proteins, peptides, lipids, and metabolites than currently exist at TAMU. The instrument will be housed within the Laboratory for Biological Mass Spectrometry (LBMS). LBMS staff scientists have extensive expertise in the operation and maintenance of commercial MS systems as well as training of a large number of MS users. It is estimated that the proposed instrument is greater than two orders of magnitude more sensitive with greater functionality when compared to similar instrumentation housed in the LBMS (a 13 year old QSTAR Pulsar in desperate need of upgrading). Moreover, in our evaluation of available instruments the 5600 was superior to all others. The 5600 offers substantial advances in terms of mass resolution, mass accuracy, linear dynamic range, and throughput over our current instrumentation. The exceptional sensitivity and utility of these instruments (described in detail below) will provide capabilities that are currently lacking in the LBMS; these capabilities are essential for the research by the NIH-funded research projects described in this grant, and these capabilities will directly impact the outcomes and public health significance of these projects.

描述（由申请人提供）：申请资金用于购买配备毛细管/纳米液相色谱的AB Sciex TripleTOF”5600质谱仪。该仪器将主要用于促进德克萨斯A&M大学（TAMU）系统内14名NIH资助的研究人员的研究。处于癌症研究、人类健康、结构生物学和药物发现等令人兴奋的工作前沿的科学家都需要能够准确识别和表征重要的分子种类，并确定分子复合物之间的特定相互作用。5600将为TAMU的NIH资助研究人员提供更好的复杂生物系统分析能力，特别是蛋白质，修饰蛋白质，肽，脂质和代谢物。仪器将存放在生物质谱实验室（LBMS）内。LBMS的科学家在商业MS系统的操作和维护以及大量MS用户的培训方面具有广泛的专业知识。据估计，与LBMS中的类似仪器（迫切需要升级的13岁QSTAR脉冲星）相比，拟议仪器的灵敏度超过两个数量级，功能更强。此外，在我们对现有仪器的评估中，5600上级所有其他仪器。5600在质量分辨率、质量精度、线性动态范围和吞吐量方面都比我们目前的仪器有了很大的进步。这些仪器的特殊敏感性和实用性（下文详细描述）将提供LBMS目前缺乏的能力;这些能力对于NIH资助的研究项目的研究至关重要，这些能力将直接影响这些项目的结果和公共卫生意义。